2. GPCR/G Protein MAPK/ERK Pathway
  3. Ras
  4. CID-1067700

CID-1067700 (ML282) is a pan GTPase inhibitor, and competitively inhibits Ras-related in brain 7 (Rab7) with a Ki of 13 nM.

For research use only. We do not sell to patients.

CAS No. : 314042-01-8

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Based on 14 publication(s) in Google Scholar

CID-1067700 Related Antibodies

Top Publications Citing Use of Products

    CID-1067700 purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 Dec 11:1-18.  [Abstract]

    Western blot analysis of SQSTM1, LC3, total RAB7A, and GTP-RAB7A (immunoprecipitated by anti-GTP-RAB7A antibody) in four groups as indicated in THP-1 cells. Cells were treated with CID-1067700 (100 μM), ML098 (100 nM), or Baf A1 (50 nM) for 8 hours.

    CID-1067700 purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 Dec 11:1-18.  [Abstract]

    GFP-RFP-LC3 THP-1 cells were treated with P. g. (MOI = 100), ML098 (100 nM), and CID-1067700 (100 μM) for 8 hours, and the colocalization of GFP-LC3 and RFP-LC3 was captured to detect autophagy. Red dots represent autolysosomes and yellow dots autophagosomes. Scale bars: 10 μm.

    CID-1067700 purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 Dec 11:1-18.  [Abstract]

    Representative micro-CT analysis of alveolar bone in normal control mice and periodontitis mice treated with ML098 (1 mg/kg, intraperitoneally injected every 2 days for 10 days), CID-1067700 (10 mg/kg, intraperitoneally injected every 2 days for 10 days), or USP4 overexpression adenovirus. Scale bars: 500 μm.

    CID-1067700 purchased from MedChemExpress. Usage Cited in: Alzheimers Res Ther. 2025 Jun 9;17(1):132.  [Abstract]

    CID1067700 (40 µM, 24 h) markedly suppressed Rab7 activity induced by the overexpression of Flag-AP2A1 in APP cells.

    CID-1067700 purchased from MedChemExpress. Usage Cited in: Alzheimers Res Ther. 2025 Jun 9;17(1):132.  [Abstract]

    CID-1067700 (40 µM, 24 h) abrogated the increase in colocalization of autophagosomes (marked by LC3) with DIC1/2 induced by overexpression of Flag-AP2A1 in APP cells.

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    Description

    CID-1067700 (ML282) is a pan GTPase inhibitor, and competitively inhibits Ras-related in brain 7 (Rab7) with a Ki of 13 nM.

    IC50 & Target

    Ki: 13 nM (Rab7)[1]
    In Vitro

    CID-1067700 (ML282) is a pan GTPase inhibitor, and competitively inhibits Rab7 with a Ki of 13 nM. CID-1067700 shows inhibitory activity against nucleotide binding by Rab7, with Kds of 100 nM and 40 nM for BODIPY-GTP and BODIPY-GDP, respectively. With increasing concentration, CID-1067700 causes strong inhibition on binding of the BODIPY-linked nucleotides, with EC50 values of 11.22 ± 1.34 nM for BODIPY-GTP and 20.96 ± 1.34 nM for BODIPY-GDP and calculated Ki values of 12.89 nM and 19.70 nM respectively. CID-1067700 (10 μM) has no effect on the rate of release of bound BODIPY-linked nucleotide by wild type Rab7 under equilibrium binding conditions[1]. CID-1067700 (0-40 μM) inhibits Rab7 activity, NF-κB activation as well as AID induction in B cells. Furthermore, CID-1067700 binds Rab7 with a high affinity (EC50: 10-20 nM), and blocks Class switch DNA recombination (CSR) in B cells via targeting Rab7[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    CID-1067700 Related Antibodies
    In Vivo

    CID-1067700 (ML282; 16 mg/kg, i.p.) prevents disease development in lupus-prone mice by Rab7 inhibition, and reduces IgG-IC deposition in MRL/Faslpr/lpr mice. CID-1067700 also targets B cells and specifically impairs the CSR machinery in vivo[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molecular Weight

    390.48

    Formula

    C18H18N2O4S2
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    O=C(C1=C(NC(NC(C2=CC=CC=C2)=O)=S)SC3=C1CC(C)(C)OC3)O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 12.5 mg/mL (32.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Ethanol : 1 mg/mL (2.56 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.5610 mL 12.8048 mL 25.6095 mL
    5 mM 0.5122 mL 2.5610 mL 5.1219 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (6.40 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
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    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.71%

    SDS (393 KB)

    COA (250 KB) HNMR (191 KB) LCMS (108 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [2]

    To treat human and mouse B cells in vitro with the Rab7 inhibitor, CID-1067700 is diluted in DMSO and added to cell cultures to the final concentration of 40 μM. CID-1067700 or DMSO is added either at the time when B cell stimulation started, or 66 h after B cells are stimulated with LPS plus IL-4, TGF-β, anti-δ/dex and RA, for analysis of plasma cell survival[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    Animal Administration
    [2]

    Mice[2]
    For in vivo treatment, CID-1067700 dissolved in DMSO (stock concentration 40 mM, 16 mg/mL) is diluted with the solvent to the final volume of 50 μL and injected intraperitoneally (i.p.) once per week at the dose of 16 mg/kg body weight. C57, MRL/Faslpr/lpr and C57/Sle1Sle2Sle3 mice injected i. p. with the vehicle DMSO (50 μL). For survival studies and skin lesion analyses, MRL/Faslpr/lpr mice are treated with nil or CID-1067700 for 10 weeks and maintained until moribund (e.g., showing signs of severe loss of mobility, hunched back, piloerection, ruffled fur, dyspnea, gasping and weight loss), at which point they are euthanized[2].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO 1 mM 2.5610 mL 12.8048 mL 25.6095 mL 64.0238 mL
    DMSO 5 mM 0.5122 mL 2.5610 mL 5.1219 mL 12.8048 mL
    10 mM 0.2561 mL 1.2805 mL 2.5610 mL 6.4024 mL
    15 mM 0.1707 mL 0.8537 mL 1.7073 mL 4.2683 mL
    20 mM 0.1280 mL 0.6402 mL 1.2805 mL 3.2012 mL
    25 mM 0.1024 mL 0.5122 mL 1.0244 mL 2.5610 mL
    30 mM 0.0854 mL 0.4268 mL 0.8537 mL 2.1341 mL
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    CID-1067700 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    CID-1067700314042-01-8ML282CID1067700CID 1067700ML 282ML-282RasGTPasecompetitivelyRas-relatedinbrain7Rab7CSRmachineryInhibitorinhibitorinhibit

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