Canertinib
Based on 9 publication(s) in Google Scholar
Canertinib (CI-1033;PD-183805) is a potent and irreversible EGFR inhibitor; inhibits cellular EGFR and ErbB2 autophosphorylation with IC50s of 7.4 and 9 nM. Canertinib is active against vaccinia virus respiratory infection in mice.
For research use only. We do not sell to patients.
- Purity: 99.74%
- CAS No.: 267243-28-7
- Formula: C24H25ClFN5O3
- Molecular Weight:485.94
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
Publications Citing Use of MedChemExpress (MCE) Canertinib
More- Sci Transl Med. 2018 Jul 18;10(450):eaaq1093. [Abstract]
- J Med Chem. 2025 Aug 28;68(16):17917-17932. [Abstract]
- Hepatol Int. 2025 Nov 3. [Abstract]
- J Biol Chem. 2012 Mar 23;287(13):9742-52. [Abstract]
- J Cell Sci. 2015 Sep 1;128(17):3317-29. [Abstract]
- Biochemistry. 2018 Feb 27;57(8):1369-1379. [Abstract]
- Biochemistry. 2017 Jun 13;56(23):2921-2927. [Abstract]
- bioRxiv. 2024 Jan 4:2023.10.06.561161. [Abstract]
- Oncotarget. 2020 Nov 3;11(44):3921-3932. [Abstract]
All EGFR Isoforms
More
Biological Activity
|
EGFR 7.4 nM (IC50) |
ErbB2 9 nM (IC50) |
|
Cell Line
|
Type | Value | Description | References |
|---|---|---|---|---|
| A-431 | IC50 |
7.4 nM
Compound: 18
|
Inhibition of EGF-stimulated autophosphorylation of EGFR enzyme in A431 cells detected by immunoblotting
Inhibition of EGF-stimulated autophosphorylation of EGFR enzyme in A431 cells detected by immunoblotting
|
[PMID: 10753475] |
| A-431 | IC50 |
0.083 μg/mL
Compound: 3 (CI-1033)
|
Inhibition of A431 cell proliferation
Inhibition of A431 cell proliferation
|
[PMID: 12270171] |
| A-431 | IC50 |
0.15 μM
Compound: CI-1033
|
Antiproliferative activity against human A431 cells overexpressing EGFR after 72 hrs by MTS assay
Antiproliferative activity against human A431 cells overexpressing EGFR after 72 hrs by MTS assay
|
[PMID: 22339342] |
| A549 | IC50 |
1.59 μM
Compound: CI-1033
|
Antiproliferative activity against human A549 cells expressing wild type EGFR coexpressing k-Ras mutant after 72 hrs by MTS assay
Antiproliferative activity against human A549 cells expressing wild type EGFR coexpressing k-Ras mutant after 72 hrs by MTS assay
|
[PMID: 22339342] |
| A549 | IC50 |
6 nM
Compound: 6; CI-1033
|
Inhibition of EGF-stimulated wild type EGFR autophosphorylation expressed in human A549 cells by sandwich ELISA
Inhibition of EGF-stimulated wild type EGFR autophosphorylation expressed in human A549 cells by sandwich ELISA
|
[PMID: 26756222] |
| A549 | IC50 |
7162 nM
Compound: Canertinib
|
Antiproliferative activity against human A549 cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay
Antiproliferative activity against human A549 cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay
|
[PMID: 33773286] |
| BaF3 | IC50 |
0.029 μM
Compound: Cl-1033
|
Inhibition of Blk expressed in mouse BAF3 cells assessed as cytotoxicity
Inhibition of Blk expressed in mouse BAF3 cells assessed as cytotoxicity
|
[PMID: 18667312] |
| BaF3 | IC50 |
0.062 μM
Compound: Cl-1033
|
Inhibition of Bmx expressed in mouse BAF3 cells assessed as cytotoxicity
Inhibition of Bmx expressed in mouse BAF3 cells assessed as cytotoxicity
|
[PMID: 18667312] |
| BaF3 | IC50 |
2 μM
Compound: Cl-1033
|
Inhibition of JAK3 expressed in mouse BAF3 cells assessed as cytotoxicity
Inhibition of JAK3 expressed in mouse BAF3 cells assessed as cytotoxicity
|
[PMID: 18667312] |
| BaF3 | IC50 |
9.2 nM
Compound: Canertinib
|
Antiproliferative activity against mouse BaF3 cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay
Antiproliferative activity against mouse BaF3 cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay
|
[PMID: 33773286] |
| BJ | IC50 |
5612 nM
Compound: Canertinib
|
Cytotoxicity against human BJ cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay
Cytotoxicity against human BJ cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay
|
[PMID: 33773286] |
| BT-474 | EC50 |
10 nM
Compound: CI-1033
|
Antiproliferative activity against human BT474 cells overexpressing ERBb2 after 3 days by methylene blue staining
Antiproliferative activity against human BT474 cells overexpressing ERBb2 after 3 days by methylene blue staining
|
[PMID: 19028424] |
| HCC827 | IC50 |
0.001 μM
Compound: CI-1033
|
Antiproliferative activity against human HCC827 cells harboring EGFR del E746-A750 mutant after 72 hrs by MTS assay
Antiproliferative activity against human HCC827 cells harboring EGFR del E746-A750 mutant after 72 hrs by MTS assay
|
[PMID: 22339342] |
| HCC827 | IC50 |
0.3776 nM
Compound: Canertinib
|
Antiproliferative activity against human HCC827 cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay
Antiproliferative activity against human HCC827 cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay
|
[PMID: 33773286] |
| HEK-293T | IC50 |
6 μM
Compound: Canertinib
|
Cytotoxicity against human HEK293T cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay
Cytotoxicity against human HEK293T cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay
|
[PMID: 33773286] |
| HN5 | EC50 |
50 nM
Compound: CI-1033
|
Antiproliferative activity against human HN5 cells overexpressing EGFR after 3 days by methylene blue staining
Antiproliferative activity against human HN5 cells overexpressing EGFR after 3 days by methylene blue staining
|
[PMID: 19028424] |
| L02 | IC50 |
2.3 μM
Compound: CI-1033
|
Antiproliferative activity against human HL7702 cells expressing wilt type EGFR after 72 hrs by MTS assay
Antiproliferative activity against human HL7702 cells expressing wilt type EGFR after 72 hrs by MTS assay
|
[PMID: 22339342] |
| NCI-H1975 | IC50 |
0.064 μM
Compound: CI-1033
|
Antiproliferative activity against human NCI-H1975 cells harboring EGFR L858R/T790M mutant after 72 hrs by MTS assay
Antiproliferative activity against human NCI-H1975 cells harboring EGFR L858R/T790M mutant after 72 hrs by MTS assay
|
[PMID: 22339342] |
| NCI-H1975 | IC50 |
3 nM
Compound: 6; CI-1033
|
Inhibition of EGFR L858R/T790M double mutant autophosphorylation in human NCI-H1975 cells after 2 hrs by sandwich ELISA
Inhibition of EGFR L858R/T790M double mutant autophosphorylation in human NCI-H1975 cells after 2 hrs by sandwich ELISA
|
[PMID: 26756222] |
| NCI-H1975 | IC50 |
101 nM
Compound: 35
|
Antiproliferative activity against human NCI-H1975 cells expressing EGFR T790M/L858R mutant incubated for 72 hrs by MTS assay
Antiproliferative activity against human NCI-H1975 cells expressing EGFR T790M/L858R mutant incubated for 72 hrs by MTS assay
|
[PMID: 28754471] |
| NCI-H1975 | IC50 |
11.34 nM
Compound: Canertinib
|
Antiproliferative activity against human NCI-H1975 cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay
Antiproliferative activity against human NCI-H1975 cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay
|
[PMID: 33773286] |
| PC-9 | IC50 |
0.8585 nM
Compound: Canertinib
|
Antiproliferative activity against human PC-9 cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay
Antiproliferative activity against human PC-9 cells assessed as inhibition of cell growth measured after 72 hrs by CCK-8 assay
|
[PMID: 33773286] |
| SK-BR-3 | IC50 |
0.102 μg/mL
Compound: 3 (CI-1033)
|
Inhibition of SKBR-3 cell proliferation
Inhibition of SKBR-3 cell proliferation
|
[PMID: 12270171] |
| SW-620 | IC50 |
1.45 μg/mL
Compound: 3 (CI-1033)
|
Inhibition of SW-620 cell proliferation
Inhibition of SW-620 cell proliferation
|
[PMID: 12270171] |
| Vero | CC50 |
>10 μM
Compound: 41; CI-1033
|
Cytotoxicity against african green monkey Vero cells
Cytotoxicity against african green monkey Vero cells
|
[PMID: 33539089] |
Canertinib significantly inhibits growth of cultured melanoma cells, RaH3 and RaH5, in a dose-dependent manner. IC50 is approximately 0.8 μM and by 5μM both cell lines are completely growth-arrested within 72 h of treatment. Incubation of exponentially growing RaH3 and RaH5 with 1 μM canertinib accumulated the cells in the G1-phase of the cell cycle within 24 h of treatment without induction of apoptosis. 1 μM canertinib inhibits ErbB1-3 receptor phosphorylation with a concomitant decrease of Akt-, Erk1/2- and Stat3 activity in both cell lines[2].
Canertinib also is a potent activator of exosome secretion[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 267243-28-7
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Appearance Solid
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Molecular Weight 485.94
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Formula C24H25ClFN5O3
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Color Light yellow to yellow
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SMILES
O=C(NC1=C(C=C2C(C(NC3=CC=C(C(Cl)=C3)F)=NC=N2)=C1)OCCCN4CCOCC4)C=C
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Synonyms
CI-1033; PD-183805
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (9)
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Journal Impact Factor
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Most Recent
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Sci Transl Med
PP2A inhibition is a druggable MEK inhibitor resistance mechanism in KRAS-mutant lung cancer cells. [Abstract]2018 Jul 18;10(450):eaaq1093. PMID: 30021885 -
J Med Chem
2025 Aug 28;68(16):17917-17932. PMID: 40801664 -
Hepatol Int
SOX9 promotes hepatocyte proliferation via upregulating TGF-α expression during liver regeneration. [Abstract]2025 Nov 3. PMID: 41182562 -
J Biol Chem
Kinome-wide selectivity profiling of ATP-competitive mammalian target of rapamycin (mTOR) inhibitors and characterization of their binding kinetics. [Abstract]2012 Mar 23;287(13):9742-52. PMID: 22223645 -
J Cell Sci
Crucial role of TRPC6 in maintaining the stability of HIF-1α in glioma cells under hypoxia. [Abstract]2015 Sep 1;128(17):3317-29. PMID: 26187851 -
Biochemistry
Discovery of an Irreversible and Cell-Active BCL6 Inhibitor Selectively Targeting Cys53 Located at the Protein-Protein Interaction Interface. [Abstract]2018 Feb 27;57(8):1369-1379. PMID: 29293322 -
Biochemistry
Universal and Quantitative Method To Evaluate Inhibitor Potency for Cysteinome Proteins Using a Nonspecific Activity-Based Protein Profiling Probe. [Abstract]2017 Jun 13;56(23):2921-2927. PMID: 28520393 -
bioRxiv
Structural dynamics of the active HER4 and HER2/HER4 complexes is finely tuned by different growth factors and glycosylation. [Abstract]2024 Jan 4:2023.10.06.561161. PMID: 38260342 -
Oncotarget
2020 Nov 3;11(44):3921-3932. PMID: 33216841
Solvent & Solubility
Ethanol : 12.5 mg/mL (25.72 mM; Need ultrasonic)
DMSO : 4.9 mg/mL (10.08 mM; Need warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.57 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (2.57 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 1.25 mg/mL (2.57 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (12.5 mg/mL) to 900 μL Corn oil, and mix evenly.
Please enter the basic information of animal experiments:
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-
-
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Enzyme assays for IC50 determinations are performed in 96-well filter plates. The total volume is 0.1 mL containing 20 mM Hepes, pH 7.4, 50 mM sodium vanadate, 40 mM magnesium chloride, 10 µM adenosine triphosphate (ATP) containing 0.5 mCi of [32P]ATP, 20 mg of polyglutamic acid/tyrosine, 10 ng of EGFR tyrosine kinase, and appropriate dilutions of inhibitor (Canertinib). All components except the ATP are added to the well and the plate is incubated with shaking for 10 min at 25°C. The reaction is started by adding [32P]ATP, and the plate is incubated at 25°C for 10 min. The reaction is terminated by addition of 0.1 mL of 20% trichloroacetic acid (TCA). The plate is kept at 4°C for at least 15 min to allow the substrate to precipitate. The wells is then washed five times with 0.2 mL of 10% TCA and 32P incorporation determined with a plate counter[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
RaH3 and RaH5 cells are treated with increasing concentrations (0-10 μM) of Canertinib for 72 h. The cells are suspended in buffer and counted[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice: Canertinib treatment starts when the tumors show reliable growth. The mice are randomized into control and treatment groups. In the canertinib treated RaH3 group (n=4) and RaH5 group (n=7) each mouse receives i.p. injections of 1.2 mg canertinib (40 mg/kg/day) in 0.1 ml 0.15 M NaCl 5 days a week. The control RaH3 (n=3) and RaH5 (n=7) mice receive i.p. injections of vehicle only according to the same regimen. At the end of the treatment period, the mice are sacrificed by cervical dislocation where after the tumors are removed and weighed[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Smaill JB, et al. Tyrosine kinase inhibitors. 17. Irreversible inhibitors of the epidermal growth factor receptor: 4-(phenylamino)quinazoline- and 4-(phenylamino)pyrido[3,2-d]pyrimidine-6-acrylamides bearing additional solubilizing functions. J Med Chem. 2000 Apr 6;43(7):1380-97. [Content Brief]
[2]. Djerf Severinsson EA, et al. The pan-ErbB receptor tyrosine kinase inhibitor canertinib promotes apoptosis of malignant melanoma in vitro and displays anti-tumor activity in vivo. Biochem Biophys Res Commun. 2011 Oct 28;414(3):563-8. [Content Brief]
[3]. McAndrews KM, et, al. Mechanisms associated with biogenesis of exosomes in cancer. Mol Cancer. 2019 Mar 30;18(1):52. [Content Brief]
[4]. Smee DF, et, al. Progress in the discovery of compounds inhibiting orthopoxviruses in animal models. Antivir Chem Chemother. 2008;19(3):115-24. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / Ethanol | 1 mM | 2.0579 mL | 10.2893 mL | 20.5787 mL | 51.4467 mL |
| 5 mM | 0.4116 mL | 2.0579 mL | 4.1157 mL | 10.2893 mL | |
| 10 mM | 0.2058 mL | 1.0289 mL | 2.0579 mL | 5.1447 mL | |
| Ethanol | 15 mM | 0.1372 mL | 0.6860 mL | 1.3719 mL | 3.4298 mL |
| 20 mM | 0.1029 mL | 0.5145 mL | 1.0289 mL | 2.5723 mL | |
| 25 mM | 0.0823 mL | 0.4116 mL | 0.8231 mL | 2.0579 mL |