1. Cell Cycle/DNA Damage
  2. Topoisomerase

Ellipticine 

Cat. No.: HY-15753
Handling Instructions

Ellipticine hydrochloride is a potent antineoplastic agent; inhibits DNA topoisomerase II activities.

For research use only. We do not sell to patients.

Ellipticine Chemical Structure

Ellipticine Chemical Structure

CAS No. : 519-23-3

Size Price Stock
5 mg USD 60 Get quote
10 mg USD 90 Get quote
50 mg USD 372 Get quote
100 mg USD 660 Get quote

* Please select Quantity before adding items.

Other In-stock Forms of Ellipticine:

Customer Review

Other Forms of Ellipticine:

View All Topoisomerase Isoform Specific Products:

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Ellipticine hydrochloride is a potent antineoplastic agent; inhibits DNA topoisomerase II activities.

IC50 & Target[1]

Topoisomerase II

 

In Vitro

Ellipticine is a potent antineoplastic agent exhibiting the multimodal mechanism of its action. The mechanisms of ellipticine antitumor, mutagenic and cytotoxic activities are suggested to be intercalation into DNA and inhibition of DNA topoisomerase II activity. Another mode of ellipticine action is the formation of covalent DNA adducts mediated by its oxidation with cytochromes P450 (CYP) and peroxidases[1]. Ellipticine can also act as an inhibitor or inducer of biotransformation enzymes, thereby modulating its own metabolism leading to its genotoxic and pharmacological effects. Treatment of cells with ellipticine results in inhibition of cell growth and proliferation. This effect is associated with formation of two covalent ellipticine-derived DNA adducts[2].

In Vivo

Ellipticine treatment results in ellipticine-derived DNA adduct generation in several healthy organs (liver, kidney, lung, spleen, breast, heart and brain) and in DNA of mammary adenocarcinoma. The levels of ellipticine-derived DNA adducts generated in these adenocarcinomas are almost 2-fold higher than in normal healthy mammary tissue. The induced expression of cytochrome b5 protein in liver of rats treated with ellipticine suggests that cytochrome b5 may modulate the CYP-mediated bioactivation and detoxification of ellipticine[3].

References
Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 4.0599 mL 20.2996 mL 40.5992 mL
5 mM 0.8120 mL 4.0599 mL 8.1198 mL
10 mM 0.4060 mL 2.0300 mL 4.0599 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay
[2]

The cytotoxicity of ellipticine is determined by MTT test. Ellipticine is dissolved in DMSO (1 mM) and diluted in culture medium to final concentrations of 0, 0.1, 1, 5 or 10 μM. Cells in exponential growth are seeded at 1×104 per well in a 96-well microplate. After incubation the MTT solution is added, the microplates are incubated for 4 hours and cells lysed in 50% N,N-dimethylformamide containing 20% of sodium dodecyl sulfate (SDS), pH 4.5. The absorbance at 570 nm is measured. The mean absorbance of medium controls is subtracted as a background. The viability of control cells is taken as 100% and the values of treated cells are calculated as a percentage of control. The IC50 values are calculated using linear regression of the dose-log response curves[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

246.31

Formula

C₁₇H₁₄N₂

CAS No.

519-23-3

SMILES

CC1=C(C=NC=C2)C2=C(C)C(N3)=C1C4=C3C=CC=C4

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: 5.8 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

Inquiry Online

Your information is safe with us. * Required Fields.

Product name

 

Salutation

Applicant name *

 

Email address *

Phone number *

 

Organization name *

Country or Region *

 

Requested quantity *

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
Ellipticine
Cat. No.:
HY-15753
Quantity: