2. MAPK/ERK Pathway
    NF-κB
  3. JNK
    NF-κB
  4. Isovitexin

Isovitexin  (Synonyms: Saponaretin; Homovitexin)

Cat. No.: HY-N0773 Purity: 99.77%
COA Handling Instructions

Isovitexin is a flavonoid isolated from passion flower, Cannabis and, and the palm, possesses anti-inflammatory and anti-oxidant activities; Isovitexin acts like a JNK1/2 inhibitor and inhibits the activation of NF-κB.

For research use only. We do not sell to patients.

Isovitexin Chemical Structure

Isovitexin Chemical Structure

CAS No. : 38953-85-4

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 133 In-stock
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
 USD 133 In-stock
Solid
5 mg USD 121 In-stock
10 mg USD 176 In-stock
25 mg USD 352 In-stock
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 3 publication(s) in Google Scholar

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  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

Isovitexin is a flavonoid isolated from passion flower, Cannabis and, and the palm, possesses anti-inflammatory and anti-oxidant activities; Isovitexin acts like a JNK1/2 inhibitor and inhibits the activation of NF-κB.

IC50 & Target[1]

JNK1

 

JNK2

 

NF-κB

 

In Vitro

Isovitexin protects against LPS-induced oxidative damage by suppressing intracellular ROS generation, and also attenuates the effect of H2O2 on cell viability. Isovitexin (0-100 μg/mL) with LPS (2 μg/mL) is not cytotoxic to RAW 264.7 cells, but 200 μg/mL Isovitexin shows significant cytotoxicity. Isovitexin (25, 50 μg/mL) inhibits LPS-induced increases in TNF-α, IL-6, iNOS, and COX-2 levels. Isovitexin (25, 50 μg/mL) also suppresses the IκBα phosphorylation and degradation in RAW 264.7 cells, and such an effect is consistent with that of JNK1/2 inhibitor[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo

Isovitexin (50 and 100 mg/kg, i.p.) causes less severe histopathological changes in the lung sections, and reduces inflammatory cell count in LPS-induced mice. Isovitexin (50 and 100 mg/kg, i.p.) protects against LPS-induced inflammation and oxidative stress in LPS-induced ALI mice by decreasing TNF-α and IL-6 production, ROS generation, and MPO and MDA content, increasing SOD and GSH levels and effectively inhibiting the protein expression of iNOS and COX-2[1]. Isovitexin (25, 50, 100 mg/kg) dose-dependently reduces the survival rate of LPS/D-gal induced hepatic injury in mice. Isovitexin also inhibits NF-κB activation and up-regulates Nrf2 and HO-1 induced by LPS/D-gal in mice[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

432.38

Appearance

Solid

Formula

C21H20O10
CAS No.
SMILES

OC1=C(C2=O)C(OC(C3=CC=C(O)C=C3)=C2)=CC(O)=C1[[email protected]@H]([[email protected]@H]([[email protected]@H](O)[[email protected]@H]4O)O)O[[email protected]@H]4CO
Structure Classification
Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (57.82 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3128 mL 11.5639 mL 23.1278 mL
5 mM 0.4626 mL 2.3128 mL 4.6256 mL
10 mM 0.2313 mL 1.1564 mL 2.3128 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (4.81 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (4.81 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.81 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.77%

COA (251 KB) LCMS (94 KB)

Handling Instructions (2659 KB)
References
Cell Assay
[1]

Cell viability is determined by an MTT assay. RAW 264.7 cells are plated in 96-well plates (1 × 104 cells/well) and incubated with various concentrations of Isovitexin (final concentration: 0-200 μg/mL) and LPS (2 μg/mL) for 24 h. In addition, the cells are pretreated with IV (25 or 50 μg/mL) for 1 h, followed by the addition of H2O2 (300 μM). After 24 h, MTT (5 mg/mL) is added to the cells, which are then incubated for another 4 h[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration
[1]

Mice[1]
To create an ALI model, mice are randomLy divided into six groups: control (saline), Isovitexin only (100 mg/kg, dissolved in 0.5% DMSO), LPS only (0.5 mg/kg, dissolved in saline), LPS (0.5 mg/kg) + Isovitexin (50 or 100 mg/kg), and LPS (0.5 mg/kg) + dexamethasone (Dex, 5 mg/kg dissolved in saline). Isovitexin or Dex (5 mg/kg) are administered Isovitexin. After exposure to Isovitexin or Dex for 1 h, the mice are anesthetized with diethyl ether, and LPS is administered intranasally (i.n.) to induce lung injury. After LPS administration for 12 h, the animals are euthanized. Accordingly, bronchoalveolar lavage fluid (BALF) and lung tissue samples are harvested to measure cytokine levels; ROS generation; SOD, GSH, MDA and MPO activity; and COX-2, iNOS, HO-1, and Nrf2 protein expression[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

Isovitexin38953-85-4Saponaretin HomovitexinJNKNF-κBc-Jun N-terminal kinaseNuclear factor-κBNuclear factor-kappaBInhibitorinhibitorinhibit

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