2. Metabolic Enzyme/Protease
  3. HIF/HIF Prolyl-Hydroxylase
  4. KC7F2

KC7F2 is a potent hypoxia inducible factor-1 (HIF-1) pathway inhibitor with an IC50 of 20 μM in LN229-HRE-AP cells, and with potential as a cancer therapy agent.

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No. CAS : 927822-86-4

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10 mM * 1 mL in DMSO
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Revisión del cliente

Based on 20 publication(s) in Google Scholar

KC7F2 Related Antibodies

Top Publications Citing Use of Products

    KC7F2 purchased from MedChemExpress. Usage Cited in: J Ethnopharmacol. 2023 May 23:308:116289.  [Abstract]

    KC7F2 upregulates the expression of Bcl2 and downregulates the expression of Bax in L02 cells.

    KC7F2 purchased from MedChemExpress. Usage Cited in: BMC Anesthesiol. 2019 Jul 9;19(1):127.  [Abstract]

    The hCMEC/D3 cells are treated with TNF-α, propofol, and Hif-1α inhibitor (KC7F2, 10 μM). Left side shows the image of a representative Western blot for Hif-1α, VEGF, p-VEGFR-2, p-ERK, ERK and occludin. Right side is the plot of normalized ratios of optical densities. β-actin is served as internal loading control.

    Ver todos los productos específicos de isoformas HIF/HIF Prolyl-Hydroxylase:

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    HIF-1α HIF

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    Descripciòn

    KC7F2 is a potent hypoxia inducible factor-1 (HIF-1) pathway inhibitor with an IC50 of 20 μM in LN229-HRE-AP cells, and with potential as a cancer therapy agent[1].

    IC50 & Target

    IC50: 20 μM (HIF-1, LN229-HRE-AP cells)[1]
    In Vitro

    KC7F2 (15–25 μM; 0-72 hours) exhibits a clear dose-response cytotoxicity with an IC50 value of approximately 15–25 μM depending on the cell lines, and this effect is more severe under hypoxic conditions[1].
    KC7F2 (0-80 μM; 6 hours) specifically reduces the protein levels of HIF-1α in a dose-dependent manner under hypoxic conditions; strongly decrease in HIF-1α levels at concentrations above 20 μM[1].
    KC7F2 does not affect the rate of HIF-1α protein degradation[1].
    KC7F2 inhibits HIF-1α protein synthesis but not its mRNA transcription[1].
    KC7F2 represses the phosphorylation of eukaryotic initiation factor 4E binding protein 1 (4EBP1)[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    KC7F2 Related Antibodies

    Cell Cytotoxicity Assay[1]

    Cell Line: MCF7 cells, LNZ308 cells, A549 cells, U251MG cells, LN229 cells
    Concentration: 15–25 μM
    Incubation Time: 0-72 hours
    Result: With cytotoxicity more pronounced in tumor cell lines as compared to normal cells.

    Cell Viability Assay[1]

    Cell Line: LN229 cells
    Concentration: 6 hours
    Incubation Time: 0 μM, 5 μM,7.5 μM,10 μM,15 μM,20 μM,30 μM,40 μM,60 μM,80 μM
    Result: Decreases HIF-1α protein levels in a dose-dependent manner.
    Peso molecular

    570.38

    Fòrmula

    C16H16Cl4N2O4S4
    No. CAS
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=S(C1=CC(Cl)=CC=C1Cl)(NCCSSCCNS(C2=CC(Cl)=CC=C2Cl)(=O)=O)=O
    Envío

    Room temperature in continental US; may vary elsewhere.
    Almacenamiento
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvente y solubilidad
    In Vitro: 

    DMSO : ≥ 32 mg/mL (56.10 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.7532 mL 8.7661 mL 17.5322 mL
    5 mM 0.3506 mL 1.7532 mL 3.5064 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Calculadora de molaridad

    • Calculadora de dilución

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.5 mg/mL (4.38 mM); Clear solution; Need ultrasonic

      This protocol yields a clear solution of 2.5 mg/mL.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (4.38 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
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    % 
    +
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    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Pureza y Documentación
    Referencias

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.7532 mL 8.7661 mL 17.5322 mL 43.8304 mL
    5 mM 0.3506 mL 1.7532 mL 3.5064 mL 8.7661 mL
    10 mM 0.1753 mL 0.8766 mL 1.7532 mL 4.3830 mL
    15 mM 0.1169 mL 0.5844 mL 1.1688 mL 2.9220 mL
    20 mM 0.0877 mL 0.4383 mL 0.8766 mL 2.1915 mL
    25 mM 0.0701 mL 0.3506 mL 0.7013 mL 1.7532 mL
    30 mM 0.0584 mL 0.2922 mL 0.5844 mL 1.4610 mL
    40 mM 0.0438 mL 0.2192 mL 0.4383 mL 1.0958 mL
    50 mM 0.0351 mL 0.1753 mL 0.3506 mL 0.8766 mL
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    KC7F2 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    KC7F2927822-86-4HIF/HIF Prolyl-HydroxylaseHypoxia-inducible factorsHIFsHIF-PHInhibitorinhibitorinhibit

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