MAFP (Synonyms: Methyl Arachidonyl Fluorophosphonate)

Art. -Nr.: HY-103334 Reinheit: 98.6%: Data Sheet
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MAFP (Methyl Arachidonyl Fluorophosphonate) is an selective, active-site directed and irreversible inhibitor of cPLA2 and iPLA2. MAFP is also a potent irreversible inhibitor of anandamide amidase.

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MAFP Chemische Struktur

CAS. Nr. : 188404-10-6

Größe	Verfügbarkeit	Menge
Solvent
1 mg (26.99 mM * 100 μL in Methyl acetate)	Auf Lager	Estimated Time of Arrival: December 31
Solvent
5 mg (26.99 mM * 500 μL in Methyl acetate)	Auf Lager	Estimated Time of Arrival: December 31
Solvent
10 mg (26.99 mM * 1 mL in Methyl acetate)	Auf Lager	Estimated Time of Arrival: December 31
Solvent
25 mg (26.99 mM * 2.5 mL in Methyl acetate)	Auf Lager	Estimated Time of Arrival: December 31
Solvent
50 mg (26.99 mM * 5 mL in Methyl acetate)	Auf Lager	Estimated Time of Arrival: December 31
Solvent
100 mg (26.99 mM * 10 mL in Methyl acetate)	Auf Lager	Estimated Time of Arrival: December 31

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Based on 5 publication(s) in Google Scholar

Other Forms of MAFP:

MAFP (Standard) Angebot einholen

5 Publications Citing Use of MCE MAFP

Spezialitätsempfehlungen

•Related Small Molecules:

•Related Proteins:

Alle Phospholipase Isoform-spezifische Produkte anzeigen:

Alle Isoformen anzeigen

Phospholipase A PLD Phospholipase C

Biologische Aktivität
Protokoll
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Beschreibung	MAFP (Methyl Arachidonyl Fluorophosphonate) is an selective, active-site directed and irreversible inhibitor of cPLA2 and iPLA2. MAFP is also a potent irreversible inhibitor of anandamide amidase.
IC₅₀ & Target	cPLA2, iPLA2^[1], Anandamide amidase^[2]
In Vitro	MAFP inhibits iPLA2, in a concentration-dependent manner with an IC₅₀ of 0.5 μM after a 5 min preincubation at 40°C in P388D1 cells. cPLA, is a phospholipid hydrolase using the hydroxyl of serine-228 residue as its catalytic nucleophile^[1]. MAFP is also an inhibitor of anandamide amidase and as a ligand for the CB1 cannabinoid receptor. MAFP demonstrates selectivity towards anandamide amidase for which it is approximately 3000 and 30000-fold more potent than it is towards chymotrypsin and trypsin, respectively. MAFP displaces [³H]CP-55940 binding to the CB1 cannabinoid receptor with an IC₅₀ of 20 nM vs 40 nM for anandamide^[2]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. MAFP Related Antibodies
Molekulargewicht	370.48
Formel	C₂₁H₃₆FO₂P
CAS. Nr.	188404-10-6
Appearance	Liquid (Density: 0.960±0.06 g/cm³)
Color	Colorless to light yellow
SMILES	CCCCC/C=C\C/C=C\C/C=C\C/C=C\CCCCP(OC)(F)=O
Versand	Shipping with dry ice.
Speicherung	-80°C, protect from light
Reinheit & Dokumentation	Purity: 98.6% Select Batch: Data Sheet (266 KB) SDS (604 KB) COA (237 KB) Handling Instructions (2659 KB)
Verweise	[1]. Lio YC, et al. Irreversible inhibition of Ca(2+)-independent phospholipase A2 by methyl arachidonyl fluorophosphonate. Biochim Biophys Acta. 1996 Jul 12;1302(1):55-60. [Content Brief] [2]. Deutsch DG, et al. Methyl arachidonyl fluorophosphonate: a potent irreversible inhibitor of anandamide amidase. Biochem Pharmacol. 1997 Feb 7;53(3):255-60. [Content Brief]

Kinase-Assay ^[1]	MAFP is dissolved and diluted in DMSO. To investigate the reversibility of iPLA 2 inhibition by MAFP, the P388D1 iPLA 2 is first concentrated approximately 10-fold using a Centricon-10 concentrator from Amicon. The concentrated iPLA 2 (20 μL) is then preincubated with either 80 μM MAFP in DMSO or DMSO alone (2 μL) for 5 min at 40°C. A 2 μL aliquot is removed and subsequently diluted 1500-fold into 3 mL of assay mixture containing 100 μM DPPC (200000 cpm per 50 μL assay mixture), 400 μM Triton X-100, 100 mM Hepes (pH 7.5), 5 mM EDTA, 1 mM DTT and 0.8 mM ATP. At the indicated time points, a 50 μL aliquot is removed and the remaining enzyme activity is quantified^[1]. MCE hat die Genauigkeit dieser Methoden nicht unabhängig bestätigt. Sie dienen nur als Referenz.
Zellassay ^[2]	Inhibition of anandamide amidase in cell culture is measured using approximately 1x10⁶ Nl8TG2 intact neuroblastoma cells. Experimental cells are preincubated for 20 min in 1.5 mL medium, consisting of Fl2/DMEM with penicillin, streptomycin, gentamicin, 10% bovine calf serum, plus MAFP (1, 5, 10, 20 nM). Control cells contained no inhibitor. Arachidonoyl is then added and the incubation continued for I hr. The amount of [³H]anandamide in the cells is quantified by liquid scintillation counting of the silica scraped from the appropriate areas of the TLC plate identified by exposure to X-ray film^[2]. MCE hat die Genauigkeit dieser Methoden nicht unabhängig bestätigt. Sie dienen nur als Referenz.
Verweise	[1]. Lio YC, et al. Irreversible inhibition of Ca(2+)-independent phospholipase A2 by methyl arachidonyl fluorophosphonate. Biochim Biophys Acta. 1996 Jul 12;1302(1):55-60. [Content Brief] [2]. Deutsch DG, et al. Methyl arachidonyl fluorophosphonate: a potent irreversible inhibitor of anandamide amidase. Biochem Pharmacol. 1997 Feb 7;53(3):255-60. [Content Brief]

MAFP Related Classifications

Neurological Disease Metabolic Disease Cardiovascular Disease

Molaritätsrechner
Verdünnungsrechner

Die Formel zur Berechnung von Molaritäten

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass		Concentration		Volume		Molecular Weight *
	=		×		×

The dilution calculator equation

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Diese Gleichung wird häufig abgekürzt als: C₁V₁ = C₂V₂

Concentration _(start)	×	Volume _(start)	=	Concentration _(final)	×	Volume _(final)
	×		=		×
C1		V1		C2		V2

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

MAFP188404-10-6Methyl Arachidonyl FluorophosphonatePhospholipaseInhibitorinhibitorinhibit

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MAFP (Synonyms: Methyl Arachidonyl Fluorophosphonate)

Kundenbewertung

Other Forms of MAFP:

MAFP Related Antibodies

5 Publications Citing Use of MCE MAFP

Spezialitätsempfehlungen

•Related Small Molecules:

•Related Proteins:

Alle Phospholipase Isoform-spezifische Produkte anzeigen:

Biologische Aktivität

Protokoll

Reinheit & Dokumentation

Verweise

Kundenbewertung

MAFP Related Antibodies

MAFP Related Classifications

Molaritätsrechner

Verdünnungsrechner

Die Formel zur Berechnung von Molaritäten

The dilution calculator equation

Keywords:

Ihre kürzlich angesehenen Produkte:

Kundenbewertung

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