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  4. 7-Methyl-6-thioguanosine

7-Methyl-6-thioguanosine  (Synonyms: MESG)

Cat. No.: HY-D0995 Purity: 99.0%
COA
SDS
Handling Instructions Technical Support

7-Methyl-6-thioguanosine (MESG) is a chromogenic substrate that can be converted to 7-methyl-6-thioguanine in the presence of purine nucleoside phosphorylase and inorganic phosphate. 7-Methyl-6-thioguanosine can be used to quantify inorganic phosphate. 7-Methyl-6-thioguanosine can also be used to determine the activities of purine nucleoside phosphorylase and protein phosphatases.

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7-Methyl-6-thioguanosine

7-Methyl-6-thioguanosine 화학구조

CAS No. : 55727-10-1

사이즈 가격 재고 수량
5 mg 해외재고보유
10 mg 해외재고보유
25 mg 해외재고보유
50 mg   견적 받기  
100 mg   견적 받기  

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

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고객리뷰

Based on 2 publication(s) in Google Scholar

7-Methyl-6-thioguanosine Related Antibodies

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제품 설명

7-Methyl-6-thioguanosine (MESG) is a chromogenic substrate that can be converted to 7-methyl-6-thioguanine in the presence of purine nucleoside phosphorylase and inorganic phosphate. 7-Methyl-6-thioguanosine can be used to quantify inorganic phosphate. 7-Methyl-6-thioguanosine can also be used to determine the activities of purine nucleoside phosphorylase and protein phosphatases[1][2].

In Vitro

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Brief Steps for Assaying Protein Phosphatases[1]:
1. Reagent Preparation: Prepare a stock solution of 7-Methyl-6-thioguanosine in DMSO for use on the same day. Determine its concentration based on the absorbance value at 331 nm and the corresponding molar extinction coefficient.
2. Reaction System Setup: Prepare the reaction mixture according to the type of protein phosphatase to be detected.
3. Reaction Detection: Equilibrate the reaction mixture at 25°C for 5 minutes, then add the protein phosphatase to initiate the reaction. Record the absorbance changes at 360 nm using a double-beam spectrophotometer. Determine the initial reaction rate by analyzing the slope of the reaction curve with software, and quantify the released phosphate based on a specific extinction coefficient.
4. Data Analysis: Process the data using a non-linear regression analysis program, calculate parameters such as Km, Vmax to evaluate the activity of the protein phosphatase.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

7-Methyl-6-thioguanosine Related Antibodies
Clinical Trial
분자량

313.33

화학식

C11H15N5O4S
CAS No.
Appearance

Solid

Color

White to yellow

SMILES

S=C1NC(N)=NC2=C1[N+](C)=CN2[C@H]3[C@@H]([C@@H]([C@@H](CO)O3)O)[O-]
선적

Room temperature in continental US; may vary elsewhere.
보관

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

용액&용해도
In Vitro: 

DMSO : ≥ 250 mg/mL (797.88 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H2O : ≥ 125 mg/mL (398.94 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.1915 mL 15.9576 mL 31.9152 mL
5 mM 0.6383 mL 3.1915 mL 6.3830 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

  • 몰농도 계산기

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
순도&문서

Purity: 99.0%

SDS (252 KB)

COA (313 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O / DMSO 1 mM 3.1915 mL 15.9576 mL 31.9152 mL 79.7881 mL
5 mM 0.6383 mL 3.1915 mL 6.3830 mL 15.9576 mL
10 mM 0.3192 mL 1.5958 mL 3.1915 mL 7.9788 mL
15 mM 0.2128 mL 1.0638 mL 2.1277 mL 5.3192 mL
20 mM 0.1596 mL 0.7979 mL 1.5958 mL 3.9894 mL
25 mM 0.1277 mL 0.6383 mL 1.2766 mL 3.1915 mL
30 mM 0.1064 mL 0.5319 mL 1.0638 mL 2.6596 mL
40 mM 0.0798 mL 0.3989 mL 0.7979 mL 1.9947 mL
50 mM 0.0638 mL 0.3192 mL 0.6383 mL 1.5958 mL
60 mM 0.0532 mL 0.2660 mL 0.5319 mL 1.3298 mL
80 mM 0.0399 mL 0.1995 mL 0.3989 mL 0.9974 mL
100 mM 0.0319 mL 0.1596 mL 0.3192 mL 0.7979 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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7-Methyl-6-thioguanosine Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

7-Methyl-6-thioguanosine55727-10-1MESGFluorescent Dyechromogenic substratepurine nucleoside phosphorylaseprotein phosphatasesInhibitorinhibitorinhibit

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상품명:
7-Methyl-6-thioguanosine
Cat. No.:
HY-D0995
수량:
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