Nigericin sodium salt

Name: Nigericin sodium salt
Brand: MedChemExpress
SKU: HY-100381
Rating: 5.0 (89 reviews)

Cat. No.: HY-100381 Purity: 98.94%: FAQs
Data Sheet SDS COA Handling Instructions

Nigericin sodium salt is an antibiotic from Streptomyces hygroscopicus that works by acting as an H⁺, K⁺, and Pb²⁺ ionophore, a NLRP3 activator.

For research use only. We do not sell to patients.

Nigericin sodium salt Chemical Structure

CAS No. : 28643-80-3

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5 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 150	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 218	In-stock	Estimated Time of Arrival: December 31
50 mg	USD 290	In-stock	Estimated Time of Arrival: December 31
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Customer Review

Based on 89 publication(s) in Google Scholar

Other Forms of Nigericin sodium salt:

Nigericin Get quote

86 Publications Citing Use of MCE Nigericin sodium salt

: Nigericin sodium salt purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2023 Mar 5;113:154743. [Abstract]; Priming with LPS (1 μg/mL; 4 h) and activating with Nigericin (10 μM; 2 h) induces NLRP3 expression, caspase-1 cleavage, and GSDMD cleavage in BMDMs.

: Nigericin sodium salt purchased from MedChemExpress. Usage Cited in: Environ Toxicol. 2023 Mar 29. [Abstract]; Nigericin (1.38mM; 2 μg/2 μL; 10 min) signifcantly increases the expression of IL-1β, IL-18, and GSDMD N-terminal in rats

: Nigericin sodium salt purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2020 Jul;24(14):8078-8090. [Abstract]; LPS + Nigericin is used to induce pyroptosis as a positive control group.

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Nigericin sodium salt is an antibiotic from Streptomyces hygroscopicus that works by acting as an H⁺, K⁺, and Pb²⁺ ionophore, a NLRP3 activator^[1].

IC₅₀ & Target

NLRP3

In Vitro

Nigericin (0.1 μM) decreases inhibits proliferation and clonogenicity of H460 lung cancer cells in a dose dependent manner. Nigericin inhibits migration and invasion of H460 lung cancer cells^[1]. Nigericin (0.1-10 nM) has apparently a dual effect on cell volume, that is a shrinking effect at lower Nigericin concentrations and a swelling effect at higher concentrations. Nigericin (0.1-1 nM) significantly decreases cytosolic pH (pHi), and slightly increases the pHi at 5 and 10 nM^[2]. Nigericin exhibits higher toxicity on S18 cells than S26 cells, with IC₅₀ of 2.03±0.55 μM and 4.77±2.35 μM, respectively. Nigericin can selectively kill cancer stem cells in NPC in vitro. Nigericin dramatically reduces the migration ability of S18 and HONE-1 cells^[3]. Nigericin exhibits gteat toxicity for the HT29 and SW116 cell line with IC₅₀ of 12.92±0.25 μmol and 15.86±0.18 μmol. Nigericin also shows a decreased ability to form colonies under anchorage-independent conditions in a standard soft agar assay^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Ngericin (4 mg/kg, i.p.) significantly reduces tumor growth and acts synergistically with the chemotherapeutic agent DDP, as shown by the tumor volumes. Nigericin markedly decreases Bmi-1 in vivo. Overexpression of Bmi-1 partially restores CSC content and metastatic ability of NPC cells under Nigericin treatment. The downregulation of Bmi-1 may be involved in the inhibitory effect of Nigericin on CSCs in NPC^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

746.94

Appearance

Solid

Formula

C₄₀H₆₇NaO₁₁

CAS No.

28643-80-3

SMILES

C[C@H]1[C@](O[C@@H]2C[C@](CC[C@@H]3C)([H])O[C@@]3([H])[C@@H](C)C(O[Na])=O)([C@@H]([C@H](OC)C2)C)O[C@](C)([C@@]4([H])O[C@@](C)([C@@]5([H])O[C@@]([C@]([C@@H](C[C@@H]6C)C)([H])O[C@]6(O)CO)([H])C[C@H]5C)CC4)C1

Structure Classification

Initial Source

Streptomyces hygroscopicus

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

Ethanol : ≥ 50 mg/mL (66.94 mM)

DMSO : 11.76 mg/mL (15.74 mM; ultrasonic and warming and adjust pH to 3 with HCl and heat to 60°C)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.3388 mL	6.6940 mL	13.3880 mL
5 mM	0.2678 mL	1.3388 mL	2.6776 mL
10 mM	0.1339 mL	0.6694 mL	1.3388 mL

*Please refer to the solubility information to select the appropriate solvent.

In Vivo:

1.

Add each solvent one by one: 10% EtOH 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.35 mM); Clear solution
2.

Add each solvent one by one: 10% EtOH 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (3.35 mM); Suspended solution; Need ultrasonic
3.

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.35 mM); Clear solution

*All of the co-solvents are available by MedChemExpress (MCE).

Purity & Documentation

Purity: 98.94%

Select Batch:

Data Sheet (282 KB) SDS (480 KB)

COA (251 KB) HNMR (467 KB) RP-HPLC (222 KB)

Handling Instructions (2659 KB)

References

[1]. Yakisich JS, et al. Nigericin decreases the viability of multidrug-resistant cancer cells and lung tumorspheres and potentiates the effects of cardiac glycosides. Tumour Biol. 2017 Mar;39(3):1010428317694310 [Content Brief]

[2]. Bissinger R, et al. Triggering of Suicidal Erythrocyte Death by the Antibiotic Ionophore Nigericin. Basic Clin Pharmacol Toxicol. 2016 May;118(5):381-9 [Content Brief]

[3]. Deng CC, et al. Nigericin selectively targets cancer stem cells in nasopharyngeal carcinoma. Int J Biochem Cell Biol. 2013 Sep;45(9):1997-2006 [Content Brief]

[4]. Zhou HM, et al. Suppression of colorectal cancer metastasis by nigericin through inhibition of epithelial-mesenchymal transition. World J Gastroenterol. 2012 Jun 7;18(21):2640-8 [Content Brief]

Cell Assay ^[1]	For RCCs, cells (appr 2000 cells/well) are plated in 96-well cell-culture microplates and incubated over nigericinht in complete media (CM)-RPMI 1640 supplemented with 5% FBS and 2 mM l-glutamine. Cells are then exposed to the appropriate concentration of drug or vehicle for 72 h. For PPSS, cells (appr 500 cells/well) are plated in 96-well cell-culture microplates incubated over Nigericinht in CM to allow them to adhere and then maintained in serum-free media for 7-8 days and then treated with the appropriate concentration of drug or vehicle for 72 h in SFM. Cell viability for cells growing under RCCs and PPSS are evaluated by the MTT assay. The absorbance of solubilized formazan is read at 570 nm using ELISA (enzyme-linked immunosorbent assay) reader. In all cases, the highest concentration of DMSO is used in the control and this concentration is maintained below 0.001% (v/v). This DMSO concentration does not show any significant antiproliferative effect on the cell line in a short-term assay. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[3]	Mice^[3] The S18 cells are injected near the scapula of the nude mice. Nine days after injection, the mice are randomly divided into four groups with six animals each (control, DDP, Nigericin and DDP combined with Nigericin). DDP (2.5 mg/kg) is injected intraperitoneally for five continuous days and nigericin (4 mg/kg) is administrated intraperitoneally every two days. Tumor length and width are measured with a vernier caliper every other day. Tumor volume is calculated using the formula V=0.5×(length×width²). The body weights of the mice are recorded every two days. Mice are humanely euthanized when the tumor volume reach 2000 mm³. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Yakisich JS, et al. Nigericin decreases the viability of multidrug-resistant cancer cells and lung tumorspheres and potentiates the effects of cardiac glycosides. Tumour Biol. 2017 Mar;39(3):1010428317694310 [Content Brief] [2]. Bissinger R, et al. Triggering of Suicidal Erythrocyte Death by the Antibiotic Ionophore Nigericin. Basic Clin Pharmacol Toxicol. 2016 May;118(5):381-9 [Content Brief] [3]. Deng CC, et al. Nigericin selectively targets cancer stem cells in nasopharyngeal carcinoma. Int J Biochem Cell Biol. 2013 Sep;45(9):1997-2006 [Content Brief] [4]. Zhou HM, et al. Suppression of colorectal cancer metastasis by nigericin through inhibition of epithelial-mesenchymal transition. World J Gastroenterol. 2012 Jun 7;18(21):2640-8 [Content Brief]