Octenidine dihydrochloride
Based on 4 publication(s) in Google Scholar
Octenidine dihydrochloride is an effective antiseptic compound for skin mucous membranes and wounds.
For research use only. We do not sell to patients.
- Purity: 99.73%
- CAS No.: 70775-75-6
- Formula: C36H64Cl2N4
- Molecular Weight:623.83
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Octenidine dihydrochloride
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Biological Activity
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Caco-2 | CC50 |
2.4 μM
Compound: OCTENIDINE
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Toxicity against Caco-2 cells determined at 48 hours by intracellular ATP concentration using the CellTiter-Glo Luminescent Cell Viability Assay
Toxicity against Caco-2 cells determined at 48 hours by intracellular ATP concentration using the CellTiter-Glo Luminescent Cell Viability Assay
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10.21203/rs.3.rs-23951/v1 |
| Caco-2 | IC50 |
1.65 μM
Compound: OCTENIDINE
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Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of Caco-2 cells after 48 hours by high content imaging
Determination of IC50 values for inhibition of SARS-CoV-2 induced cytotoxicity of Caco-2 cells after 48 hours by high content imaging
|
10.21203/rs.3.rs-23951/v1 |
| SK-MEL-2 | IC50 |
0.46 μM
Compound: Octenidine
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Cytotoxicity against human SK-MEL-2 cells assessed as inhibition of cell viability treated for 72 hrs measured after 72 hrs
Cytotoxicity against human SK-MEL-2 cells assessed as inhibition of cell viability treated for 72 hrs measured after 72 hrs
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[PMID: 36961300] |
| SK-MEL-2 | IC50 |
0.48 μM
Compound: Octenidine
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Cytotoxicity against human SK-MEL-2 cells assessed as inhibition of cell viability treated for 72 hrs measured after 24 hrs
Cytotoxicity against human SK-MEL-2 cells assessed as inhibition of cell viability treated for 72 hrs measured after 24 hrs
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[PMID: 36961300] |
| SK-MEL-2 | IC50 |
0.49 μM
Compound: Octenidine
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Antiproliferative activity against human SK-MEL-2 cells assessed as cell growth inhibition incubated for 48 hrs by EdU assay
Antiproliferative activity against human SK-MEL-2 cells assessed as cell growth inhibition incubated for 48 hrs by EdU assay
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[PMID: 36961300] |
| SK-MEL-2 | IC50 |
1.08 μM
Compound: Octenidine
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Antiproliferative activity against human SK-MEL-2 cells assessed as cell growth inhibition incubated for 24 hrs by EdU assay
Antiproliferative activity against human SK-MEL-2 cells assessed as cell growth inhibition incubated for 24 hrs by EdU assay
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[PMID: 36961300] |
| SK-MEL-2 | IC50 |
1.34 μM
Compound: Octenidine
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Cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition incubated for 48 hrs by MTT assay
Cytotoxicity against human SK-MEL-2 cells expressing NRAS mutant assessed as cell growth inhibition incubated for 48 hrs by MTT assay
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[PMID: 36961300] |
| SK-MEL-2 | IC50 |
12.4 μM
Compound: Octenidine
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Cytotoxicity against human SK-MEL-2 cells expressing Tet-on NRAS Q61R assessed as cell growth inhibition in presence of doxycycline
Cytotoxicity against human SK-MEL-2 cells expressing Tet-on NRAS Q61R assessed as cell growth inhibition in presence of doxycycline
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[PMID: 36961300] |
| SK-MEL-2 | IC50 |
2.05 μM
Compound: Octenidine
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Antiproliferative activity against human SK-MEL-2 cells assessed as cell growth inhibition incubated for 12 hrs by EdU assay
Antiproliferative activity against human SK-MEL-2 cells assessed as cell growth inhibition incubated for 12 hrs by EdU assay
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[PMID: 36961300] |
| SK-MEL-2 | IC50 |
2.1 μM
Compound: Octenidine
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Cytotoxicity against human SK-MEL-2 cells assessed as inhibition of cell viability treated for 48 hrs measured after 48 hrs
Cytotoxicity against human SK-MEL-2 cells assessed as inhibition of cell viability treated for 48 hrs measured after 48 hrs
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[PMID: 36961300] |
| SK-MEL-2 | IC50 |
2.11 μM
Compound: Octenidine
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Cytotoxicity against human SK-MEL-2 cells assessed as inhibition of cell viability treated for 48 hrs measured after 24 hrs
Cytotoxicity against human SK-MEL-2 cells assessed as inhibition of cell viability treated for 48 hrs measured after 24 hrs
|
[PMID: 36961300] |
| SK-MEL-2 | IC50 |
2.34 μM
Compound: Octenidine
|
Cytotoxicity against human SK-MEL-2 cells assessed as cell growth inhibition incubated for 24 hrs by MTT assay
Cytotoxicity against human SK-MEL-2 cells assessed as cell growth inhibition incubated for 24 hrs by MTT assay
|
[PMID: 36961300] |
| SK-MEL-2 | IC50 |
6.3 μM
Compound: Octenidine
|
Cytotoxicity against human SK-MEL-2 cells expressing Tet-on NRAS Q61R assessed as cell growth inhibition
Cytotoxicity against human SK-MEL-2 cells expressing Tet-on NRAS Q61R assessed as cell growth inhibition
|
[PMID: 36961300] |
Octenidine concentrations of less than1.5 μM (0.94 μg/mL) causes a greater than 99% reduction of tested microbial population within 15 min. Staphylococcus epidermidis is the most susceptible of the test organisms, and E. coli and C. albicans are the least susceptible[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 70775-75-6
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Appearance Solid
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Molecular Weight 623.83
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Formula C36H64Cl2N4
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Color White to off-white
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SMILES
CCCCCCCC/N=C1C=CN(CCCCCCCCCCN(C=C/2)C=CC2=N/CCCCCCCC)C=C/1.Cl.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (4)
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Journal Impact Factor
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Most Recent
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Antioxidants (Basel)
Pitavastatin Calcium Confers Fungicidal Properties to Fluconazole by Inhibiting Ubiquinone Biosynthesis and Generating Reactive Oxygen Species. [Abstract]2024 May 29;13(6):667. PMID: 38929106 -
Antiviral Res
Repurposing screen using a robust human rhinovirus infectious clone identifies pyrvinium pamoate with antiviral activity. [Abstract]2026 Jun:250:106417. PMID: 42025967 -
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Solvent & Solubility
H2O : 33.33 mg/mL (53.43 mM; Need ultrasonic)
DMSO : 5 mg/mL (8.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (8.02 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (8.02 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Protocol
The bactericidal activities of OCT and CHG against Staphylococcus aureus are measured by exposing cultures to several different concentrations of each compound for various lengths of time. OCT is diluted from the 5 mM stock solution and tested in phosphate buffer at levels ranging from 0.5 to 5.0 FsM. The test is begun by adding the bacterial culture to the test solution containing OCT or CHG; samples are taken at 5, 15, 30, and 60 min afterward. The inoculum size is approximately 106 CFU/mL[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Monkeys: Four solutions of OCT are prepared by dissolving OCT in distilled water to final concentrations (wt/wt) of 0.2, 0.4, 0.8 and 1.6%, equivalent to 3.2, 6.4, 12.8 and 25.6 mM, respectively. Skin-degerming activities of aqueous and formulated octenidine and formulated chlorhexidine are compared in single and multiple applications of these agents to the hand and foot surfaces of monkeys by using a glove-juice extraction procedure to measure the skin microflora[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Sedlock DM, et al. Microbicidal activity of octenidine hydrochloride, a new alkanediylbis[pyridine] germicidal agent. Antimicrob Agents Chemother. 1985 Dec;28(6):786-90. [Content Brief]
[2]. Emilson CG, et al. Effect of the antibacterial agents octenidine and chlorhexidine on the plaque flora in primates. Scand J Dent Res. 1981 Oct;89(5):384-92. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO / H2O | 1 mM | 1.6030 mL | 8.0150 mL | 16.0300 mL | 40.0750 mL |
| 5 mM | 0.3206 mL | 1.6030 mL | 3.2060 mL | 8.0150 mL | |
| H2O | 10 mM | 0.1603 mL | 0.8015 mL | 1.6030 mL | 4.0075 mL |
| 15 mM | 0.1069 mL | 0.5343 mL | 1.0687 mL | 2.6717 mL | |
| 20 mM | 0.0802 mL | 0.4008 mL | 0.8015 mL | 2.0038 mL | |
| 25 mM | 0.0641 mL | 0.3206 mL | 0.6412 mL | 1.6030 mL | |
| 30 mM | 0.0534 mL | 0.2672 mL | 0.5343 mL | 1.3358 mL | |
| 40 mM | 0.0401 mL | 0.2004 mL | 0.4008 mL | 1.0019 mL | |
| 50 mM | 0.0321 mL | 0.1603 mL | 0.3206 mL | 0.8015 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Octenidine dihydrochloride Related Classifications
HY-B2170A Related Classifications
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