Octenidine dihydrochloride

Name: Octenidine dihydrochloride
Brand: MedChemExpress
SKU: HY-B2170A
Rating: 4.5 (1 reviews)

Cat. No.: HY-B2170A Purity: 99.85%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Octenidine dihydrochloride is an effective antiseptic compound for skin mucous membranes and wounds.

For research use only. We do not sell to patients.

Octenidine dihydrochloride Chemical Structure

CAS No. : 70775-75-6

Size	Price	Stock	Quantity
Solid + Solvent
10 mM * 1 mL in Water ready for reconstitution	USD 28	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	USD 28	In-stock	Estimated Time of Arrival: December 31
Solid
200 mg	USD 25	In-stock	Estimated Time of Arrival: December 31
1 g	USD 35	In-stock	Estimated Time of Arrival: December 31
5 g	USD 98	In-stock	Estimated Time of Arrival: December 31
10 g		Get quote
50 g		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Octenidine dihydrochloride:

Octenidine Get quote

1 Publications Citing Use of MCE Octenidine dihydrochloride

•University of Saskatchewan. 2020 Jun 22.

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Octenidine dihydrochloride is an effective antiseptic compound for skin mucous membranes and wounds.

In Vitro

Octenidine concentrations of less than1.5 μM (0.94 μg/mL) causes a greater than 99% reduction of tested microbial population within 15 min. Staphylococcus epidermidis is the most susceptible of the test organisms, and E. coli and C. albicans are the least susceptible^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

The antimicrobial activity of octenidine hydrochloride (OCT) is maintained when applied to the skin of the hands and feet of cynomolgus monkeys. Aqueous octenidine, at a concentration of 0.2 to 1.6% reduces resident microflora populations from 90 to 99.98%^[1]. A significant reduction in plaque score is observed on the buccal tooth surfaces after daily topical application of 1% solutions of octenidine and chlorhexidine for 7 d; octenidine is more effective than chlorhexidine^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

623.83

Formula

C₃₆H₆₄Cl₂N₄

CAS No.

70775-75-6

Appearance

Solid

Color

White to off-white

SMILES

CCCCCCCC/N=C1C=CN(CCCCCCCCCCN(C=C/2)C=CC2=N/CCCCCCCC)C=C/1.Cl.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : 33.33 mg/mL (53.43 mM; Need ultrasonic)

DMSO : 5 mg/mL (8.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6030 mL	8.0150 mL	16.0300 mL
5 mM	0.3206 mL	1.6030 mL	3.2060 mL
10 mM	0.1603 mL	0.8015 mL	1.6030 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (8.02 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (8.02 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 5 mg/mL (8.02 mM); Clear solution

This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 4

Add each solvent one by one: 5% DMSO 40% PEG300 5% Tween-80 50% Saline
Solubility: ≥ 2.5 mg/mL (4.01 mM); Clear solution
Protocol 5

Add each solvent one by one: 5% DMSO 95% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.01 mM); Clear solution

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.85%

Select Batch:

Data Sheet (268 KB) SDS (393 KB)

COA (239 KB) HNMR (273 KB) LCMS (267 KB)

Handling Instructions (2659 KB)

References

[1]. Sedlock DM, et al. Microbicidal activity of octenidine hydrochloride, a new alkanediylbis[pyridine] germicidal agent. Antimicrob Agents Chemother. 1985 Dec;28(6):786-90. [Content Brief]

[2]. Emilson CG, et al. Effect of the antibacterial agents octenidine and chlorhexidine on the plaque flora in primates. Scand J Dent Res. 1981 Oct;89(5):384-92. [Content Brief]

Kinase Assay ^[1]	The bactericidal activities of OCT and CHG against Staphylococcus aureus are measured by exposing cultures to several different concentrations of each compound for various lengths of time. OCT is diluted from the 5 mM stock solution and tested in phosphate buffer at levels ranging from 0.5 to 5.0 FsM. The test is begun by adding the bacterial culture to the test solution containing OCT or CHG; samples are taken at 5, 15, 30, and 60 min afterward. The inoculum size is approximately 106 CFU/mL^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[1]	Monkeys: Four solutions of OCT are prepared by dissolving OCT in distilled water to final concentrations (wt/wt) of 0.2, 0.4, 0.8 and 1.6%, equivalent to 3.2, 6.4, 12.8 and 25.6 mM, respectively. Skin-degerming activities of aqueous and formulated octenidine and formulated chlorhexidine are compared in single and multiple applications of these agents to the hand and foot surfaces of monkeys by using a glove-juice extraction procedure to measure the skin microflora^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Sedlock DM, et al. Microbicidal activity of octenidine hydrochloride, a new alkanediylbis[pyridine] germicidal agent. Antimicrob Agents Chemother. 1985 Dec;28(6):786-90. [Content Brief] [2]. Emilson CG, et al. Effect of the antibacterial agents octenidine and chlorhexidine on the plaque flora in primates. Scand J Dent Res. 1981 Oct;89(5):384-92. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	1.6030 mL	8.0150 mL	16.0300 mL	40.0750 mL
DMSO / H₂O	5 mM	0.3206 mL	1.6030 mL	3.2060 mL	8.0150 mL
H₂O	10 mM	0.1603 mL	0.8015 mL	1.6030 mL	4.0075 mL
	15 mM	0.1069 mL	0.5343 mL	1.0687 mL	2.6717 mL
	20 mM	0.0802 mL	0.4008 mL	0.8015 mL	2.0038 mL
	25 mM	0.0641 mL	0.3206 mL	0.6412 mL	1.6030 mL
	30 mM	0.0534 mL	0.2672 mL	0.5343 mL	1.3358 mL
	40 mM	0.0401 mL	0.2004 mL	0.4008 mL	1.0019 mL
	50 mM	0.0321 mL	0.1603 mL	0.3206 mL	0.8015 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.