2. Others Cell Cycle/DNA Damage
  3. Fluorescent Dye DNA/RNA Synthesis
  4. Propidium Iodide

Propidium Iodide  (Synonyms: ヨウ化プロピジウム)

製品番号: HY-D0815 純度: 99.77%
COA 取扱説明書 Technical Support

Propidium Iodide (PI) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis.

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CAS 番号 : 25535-16-4

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 47 在庫あり
Solution
10 mM * 1 mL in DMSO USD 47 在庫あり
Solid
5 mg $32 在庫あり
10 mg $50 在庫あり
25 mg $75 在庫あり
50 mg $100 在庫あり
100 mg $150 在庫あり
500 mg $500 在庫あり
1 g   お問い合わせ  
5 g   お問い合わせ  

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カスタマーレビュー

Based on 140 publication(s) in Google Scholar

Other Forms of Propidium Iodide:

Propidium Iodide 関連抗体

Top Publications Citing Use of Products

顧客検証

Cell Proliferation/Viability Assay
IF

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: ACS Nano. 2023 Feb 28;17(4):3334-3345.  [Abstract]

    Propidium iodide (PI) staining for cell death with GCFN and ferroptosis inhibitor ferrostatin-1 treatment for 48 h

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Eur J Pharmacol. 2022 Apr 5;920:174830.  [Abstract]

    Primary newborn rat cardiac fibroblasts are stained with PI (2 μg/mL; for 2 h) and analyzed using a Nikon Ti2 microscope.

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Molecules. 2022 Oct 9;27(19):6733.  [Abstract]

    In A549 cells, Propidium iodide (PI, 50 μg/mL) is added for incubation for 30 min in the dark. Cell-cycle distribution is determined using a FACSaria-I flow cytometer.

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Thorac Cancer. 2022 May;13(9):1299-1310.  [Abstract]

    KYSE450 and ECA109 cells after 96 h various transfections were washed three times in cold PBS, stained with Propidium iodide (PI, 50 μg/mL) and Annexin V‐FITC (25 μg/mL) for 20 min in the dark, and analyzed within 1 h.

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Redox Biol. 2022 Feb;49:102207.  [Abstract]

    PAMs and PK-15CD163 cells sre washed with phosphate-buffered saline (PBS), and then incubated with the nuclear dye propidium iodide for 15 min. The nuclei sre counterstained with propidium iodide (red). Fluorescent images are acquired with a confocal laser scanning microscope.

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2021 Aug 31;2021:5608133.

    The NP tissue slices are incubated with a culture medium containing 1 μg/ml Calcein AM and 1 μg/ml PI for one hour at 37°C. After washing with PBS, the slices are immediately viewed using an inverted confocal laser scanning microscope.

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Aging (Albany NY). 2018 Nov 28;10(11):3353-3370.  [Abstract]

    KLE cells are stained by 25 μg/mL PI in fluorescence-activated cell sorting buffer, incubated cells for 30 min in dark environment at room temperature. Cell apoptosis is determined by Annexin V/PI double staining and percentages of cells in each phase are calculated.

    Propidium Iodide purchased from MedChemExpress. Usage Cited in: Chinese Pharmacological Bulletin. 2018 May; 34(5): 620-626.

    PC12 cells are treated with 1.5 mM Methylglyoxal (MG) for 24 h before pretreatment with Butein (2.5, 5 μM) for 1 h. Propidium iodide (PI) and Hoechst 33342 are used to determine cell apoptosis.

    DNA/RNA Synthesis アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

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    • 純度とドキュメンテーション

    • 参考文献

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    製品説明

    Propidium Iodide (PI) is a nuclear staining agent that stains DNA. Propidium Iodide is an analogue of ethidine bromide that emits red fluorescence upon embedding in double-stranded DNA. Propidium Iodide cannot pass through living cell membranes, but it can pass through damaged cell membranes to stain the nucleus. Propidium Iodide has a fluorescence wavelength of 493/617 nm and a wavelength of 536/635 nm after Mosaic with DNA. Propidium Iodide is commonly used in the detection of apoptosis (apoptosis) or necrosis (necrosis), and is often used in flow cytometry analysis.

    Cellular Effect
    Cell Line Type Value Description References
    HEK293 IC50
    1.1 3
    Compound: propidium iodide
    Inhibition of wild type mouse AChE expressed in HEK293 cells using ATCh as substrate
    Inhibition of wild type mouse AChE expressed in HEK293 cells using ATCh as substrate
    		[PMID: 23984975]
    HEK293 IC50
    1.1 3
    Compound: propidium iodide
    Inhibition of wild type mouse AChE expressed in HEK293 cells using ATCh as substrate
    Inhibition of wild type mouse AChE expressed in HEK293 cells using ATCh as substrate
    		[PMID: 23984975]
    HEK293 IC50
    1.1 3
    Compound: propidium iodide
    Inhibition of wild type mouse AChE expressed in HEK293 cells using ATCh as substrate
    Inhibition of wild type mouse AChE expressed in HEK293 cells using ATCh as substrate
    		[PMID: 23984975]
    体外実験

    Guide (The following is our recommended solution. This solution is merely a guideline and should be modified according to your specific needs.)
    1. Prepare the storage solution and working solution

    1.1 Prepare the storage solution
    Prepare the 1 mg/mL stock solution using DDH2O.
    1.2 Preparation of working solution
    Dilute the stored solution with pre-heated serum-free cell culture medium or PBS to prepare a 20-50 μg/mL working solution of Propidium Iodide.
    Note: Please adjust the concentration of the Propidium Iodide working solution according to the actual situation, and prepare it as needed.
    2. Cell Staining (Suspension Cells)
    2.1 Centrifuge to collect the cells, then add PBS for two washes, each for 5 minutes. The cell density is 1×106 per mL.
    2.2 Add 1 mL of the working solution and incubate at room temperature for 5 to 10 minutes.
    2.3 400 grams. Centrifuge for 3-4 minutes. Discard the supernatant.
    2.4 Add PBS to wash the cells twice, for 5 minutes each time.
    2.5 After resuspending the cells in 1 mL of serum-free medium or PBS, observe them using a fluorescence microscope or a flow cytometer.
    3. Cell Staining (Monolayer Cells)
    3.1 Place the monolayer cells on a sterile cover slip.
    3.2 Remove the cover glass from the culture medium and aspirate away the excess medium.
    3.3 Add 100 μL of the dye working solution and gently shake to ensure complete coverage of the cells. Incubate for 5 to 30 minutes.
    3.4 Remove the dye working solution and wash with the culture medium 2-3 times, for 5 minutes each time. Observe under a fluorescence microscope.
    Note: If flow cytometry is to be used for detection, the cells should first be digested with trypsin, resuspended, and then stained.

    Notes
    1. Please adjust the concentration of Propidium Iodide working solution and the incubation time according to the actual situation.
    2. Only the nuclei of dead cells will be stained red. 3. This product is exclusively for professional scientific research purposes only. It shall not be used for clinical diagnosis or treatment, nor for food or medicine.
    3. Propidium Iodide is carcinogenic. For your safety and health, please wear a laboratory coat and use disposable gloves when operating.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Propidium Iodide 関連抗体
    分子量

    668.39

    分子式

    C27H34I2N4
    Emission (Em)

    615
    Excitation (Ex)

    535
    CAS 番号
    Appearance

    Solid

    Color

    Brown to red

    SMILES

    C[N+](CCC[N+]1=C(C2=CC=CC=C2)C3=CC(N)=CC=C3C4=C1C=C(N)C=C4)(CC)CC.[I-].[I-]
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (149.61 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 3.57 mg/mL (5.34 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.4961 mL 7.4807 mL 14.9613 mL
    5 mM 0.2992 mL 1.4961 mL 2.9923 mL
    10 mM 0.1496 mL 0.7481 mL 1.4961 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

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    体積 （終了）

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

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    Dosing volume
    (per animal)

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    純度とドキュメンテーション

    純度: 99.77%

    COA (249 KB) HNMR (185 KB) RP-HPLC (226 KB) MS (231 KB)

    取扱説明書 (2659 KB)
    Dyeing Example
    参考文献
    細胞実験
    [2]

    Flow cytometric analysis: Propidium iodide is prepared in in 0.1% sodium citrate plus 0.1% Triton X-100 (50 μg/mL). The 200 ×g centrifuged cell pellet is gently resuspended in 1.5 mL hypotonlc fluorochrome solution (Propidium iodide 50 μg/mL), in 12×75 polypropylene tubes. The tubes are placed at 4°C in the dark overnight before the flow cytometric analysis. The propidium Iodide fluorescence of individual nuclei is measured using a FACScan flow cytometer. The nuclei traverses the light beam of a 488 nm Argon laser. A 560 nm dichrolc nurror (DM 570) and a 600 nm band pass filter (bandwidth 35 nm) are used for collecting the red fluorescence due to propidium Iodide staining of DNA and the data are registered on a logarithmic scale[2].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (sealed storage, away from moisture and light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O / DMSO 1 mM 1.4961 mL 7.4807 mL 14.9613 mL 37.4033 mL
    5 mM 0.2992 mL 1.4961 mL 2.9923 mL 7.4807 mL
    DMSO 10 mM 0.1496 mL 0.7481 mL 1.4961 mL 3.7403 mL
    15 mM 0.0997 mL 0.4987 mL 0.9974 mL 2.4936 mL
    20 mM 0.0748 mL 0.3740 mL 0.7481 mL 1.8702 mL
    25 mM 0.0598 mL 0.2992 mL 0.5985 mL 1.4961 mL
    30 mM 0.0499 mL 0.2494 mL 0.4987 mL 1.2468 mL
    40 mM 0.0374 mL 0.1870 mL 0.3740 mL 0.9351 mL
    50 mM 0.0299 mL 0.1496 mL 0.2992 mL 0.7481 mL
    60 mM 0.0249 mL 0.1247 mL 0.2494 mL 0.6234 mL
    80 mM 0.0187 mL 0.0935 mL 0.1870 mL 0.4675 mL
    100 mM 0.0150 mL 0.0748 mL 0.1496 mL 0.3740 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Propidium Iodide Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Propidium25535-16-4Fluorescent DyeDNA/RNA SynthesisInhibitorinhibitorinhibit

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    製品名:
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    製品番号:
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