Ro 41-1049 hydrochloride
Based on 1 Customer Validation
Ro 41-1049 hydrochloride is a highly selective, reversible MAO-A inhibitor. Ro 41-1049 hydrochloride effectively blocks the Quinpirole (HY-B1752)-induced increase in dopamine D2-like receptor density in the nucleus accumbens and alters the pattern of behavioral sensitization, shifting animals' responses from enhanced motor activity to immobile self-directed gaping behavior. Tritium-labeled Ro 41-1049 hydrochloride serves as a high-affinity radioligand, enabling accurate mapping of the distribution and abundance of MAO-A in the central nervous system, peripheral organs, and human brain via quantitative enzyme autoradiography. Ro 41-1049 hydrochloride can be used for basic research on depression and other related diseases.
For research use only. We do not sell to patients.
- Purity: 99.57%
- CAS No.: 127917-66-2
- Formula: C12H13ClFN3OS
- Molecular Weight:301.77
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Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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D2 Receptor |
MAO-A |
Ro 41-1049 hydrochloride (10 μM; 4 hrs) inhibits a portion of in vitro [3H]quinpirole binding to dopamine D2-like receptors in nucleus accumbens and striatum tissue homogenates, though this inhibitory effect is not modulated by prior in vivo quinpirole or Ro 41-1049 hydrochloride treatments[1].
Tritiated Ro 41-1049 hydrochloride (1-150 nM; 60 min) binds to MAO-A in rat cerebral cortex sections with high affinity (Kd = 10.7 nM) and a maximum binding capacity of 7.38 pmol/mg protein, with consistent high-affinity binding across rat brain regions[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Long-Evans (male, 200-230 g at study start)[1]
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Dosage:1 mg/kg
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Administration:s.c.; 90 minutes prior to each quinpirole injection; twice weekly for 8 pairs of injections
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Result:Increased total mouthing after the 8th injection to 7-times higher than in saline control rats.
Reduced [3H]spiperone binding in the nucleus accumbens by 38% compared to quinpirole-sensitized rats without Ro 41-1049 pre-treatment.
Prevented the 32% increase in [3H]quinpirole and [3H]spiperone binding observed in the nucleus accumbens of quinpirole-sensitized rats, with receptor binding levels matching saline controls.
Showed no alterations in dopamine D2-like receptor binding, percentage of receptors in the high affinity state, or percentage of MAO inhibitor-inhibitable [3H]quinpirole binding in the striatum.
Resulted in distance traveled not different from saline controls.
Chemical Information
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CAS No. 127917-66-2
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Appearance Solid
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Molecular Weight 301.77
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Formula C12H13ClFN3OS
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Color White to off-white
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SMILES
O=C(C1=C(C2=CC=CC(F)=C2)SC=N1)NCCN.[H]Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : ≥ 32 mg/mL (106.04 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 25 mg/mL (82.84 mM; Need ultrasonic)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.28 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.28 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 20 mg/mL (66.28 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (281 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O / DMSO | 1 mM | 3.3138 mL | 16.5689 mL | 33.1378 mL | 82.8446 mL |
| 5 mM | 0.6628 mL | 3.3138 mL | 6.6276 mL | 16.5689 mL | |
| 10 mM | 0.3314 mL | 1.6569 mL | 3.3138 mL | 8.2845 mL | |
| 15 mM | 0.2209 mL | 1.1046 mL | 2.2092 mL | 5.5230 mL | |
| 20 mM | 0.1657 mL | 0.8284 mL | 1.6569 mL | 4.1422 mL | |
| 25 mM | 0.1326 mL | 0.6628 mL | 1.3255 mL | 3.3138 mL | |
| 30 mM | 0.1105 mL | 0.5523 mL | 1.1046 mL | 2.7615 mL | |
| 40 mM | 0.0828 mL | 0.4142 mL | 0.8284 mL | 2.0711 mL | |
| 50 mM | 0.0663 mL | 0.3314 mL | 0.6628 mL | 1.6569 mL | |
| 60 mM | 0.0552 mL | 0.2761 mL | 0.5523 mL | 1.3807 mL | |
| 80 mM | 0.0414 mL | 0.2071 mL | 0.4142 mL | 1.0356 mL | |
| DMSO | 100 mM | 0.0331 mL | 0.1657 mL | 0.3314 mL | 0.8284 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Ro 41-1049 hydrochloride Related Classifications
HY-100027A Related Classifications
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