Cat. No.: HY-N0342

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Scopoletin

(Synonyms: Gelseminic acid; Chrysatropic acid)

4 Cited Publications

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Based on 4 publication(s) in Google Scholar

Scopoletin is an inhibitor of acetylcholinesterase (AChE).

For research use only. We do not sell to patients.

Purity: 99.89%
CAS No.: 92-61-5
Formula: C₁₀H₈O₄
Molecular Weight:192.17

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Scopoletin Chemical Structure

CAS No.: 92-61-5

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Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (4)
Solvent & Solubility
- In Vitro
- In Vivo
Protocol
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

Scopoletin is an inhibitor of acetylcholinesterase (AChE).

IC₅₀ & Target

AChE

All Cholinesterase (ChE) Isoforms :

AChE
BChE

Cellular Effect

Cell Line	Type	Value	Description	References
A-431	IC₅₀	>100 μM Compound: Sco, Scopoletin	Cytotoxicity against human A431 cells after 24 hrs by MTT assay Cytotoxicity against human A431 cells after 24 hrs by MTT assay	[PMID: 23218780]
A549	IC₅₀	>100 μM Compound: Sco, Scopoletin	Cytotoxicity against human A549 cells after 24 hrs by MTT assay Cytotoxicity against human A549 cells after 24 hrs by MTT assay	[PMID: 23218780]
A549	IC₅₀	>20 μg/mL Compound: 14	Cytotoxicity against human A549 cells Cytotoxicity against human A549 cells	[PMID: 23511021]
A549	IC₅₀	>30 μM Compound: 6, Scopoletin	Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human A549 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25703298]
A549	IC₅₀	>25 μM Compound: Scopoletin	Anticancer activity against human A549 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay Anticancer activity against human A549 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay	[PMID: 32438251]
BXPC-3	IC₅₀	>100 μM Compound: Sco, Scopoletin	Cytotoxicity against human BxPC3 cells after 24 hrs by MTT assay Cytotoxicity against human BxPC3 cells after 24 hrs by MTT assay	[PMID: 23218780]
DU-145	IC₅₀	>50 μM Compound: scopoletin	Cytotoxicity against human DU145 Cytotoxicity against human DU145	[PMID: 16038556]
HCT-116	IC₅₀	>100 μM Compound: Sco, Scopoletin	Cytotoxicity against human HCT116 cells after 24 hrs by MTT assay Cytotoxicity against human HCT116 cells after 24 hrs by MTT assay	[PMID: 23218780]
HCT-116	IC₅₀	>30 μM Compound: 6, Scopoletin	Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human HCT116 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25703298]
HCT-116	IC₅₀	>25 μM Compound: Scopoletin	Growth inhibition of human HCT116 cells after 48 hrs by MTT assay Growth inhibition of human HCT116 cells after 48 hrs by MTT assay	[PMID: 27956344]
HeLa	IC₅₀	>80 μM Compound: Scopoletin	Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human HeLa cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 36242987]
Hep 3B2	IC₅₀	>13.23 μg/mL Compound: 14	Cytotoxicity against human Hep3B cells Cytotoxicity against human Hep3B cells	[PMID: 23511021]
HepG2	IC₅₀	>50 μM Compound: scopoletin	Cytotoxicity against human HepG2 Cytotoxicity against human HepG2	[PMID: 16038556]
HepG2	IC₅₀	>100 μM Compound: Sco, Scopoletin	Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay Cytotoxicity against human HepG2 cells after 24 hrs by MTT assay	[PMID: 23218780]
HepG2	IC₅₀	>20 μg/mL Compound: 14	Cytotoxicity against human HepG2 cells Cytotoxicity against human HepG2 cells	[PMID: 23511021]
HepG2	IC₅₀	>25 μM Compound: Scopoletin	Anticancer activity against human HepG2 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay Anticancer activity against human HepG2 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay	[PMID: 32438251]
HL-60	IC₅₀	9.5 μM Compound: 3	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 15387667]
HL-60	IC₅₀	>50 μM Compound: scopoletin	Cytotoxicity against human HL60 Cytotoxicity against human HL60	[PMID: 16038556]
HL-60	IC₅₀	>100 μM Compound: Sco, Scopoletin	Cytotoxicity against human HL60 cells after 24 hrs by MTT assay Cytotoxicity against human HL60 cells after 24 hrs by MTT assay	[PMID: 23218780]
HT-1080	IC₅₀	12.5 μM Compound: 3	Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay Cytotoxicity against human HT1080 cells after 72 hrs by MTT assay	[PMID: 15387667]
HT-29	IC₅₀	>100 μM Compound: 7, scopoletin	Antiproliferative activity against human HT-29 cells after 72 hrs by crystal violet staining method Antiproliferative activity against human HT-29 cells after 72 hrs by crystal violet staining method	[PMID: 22765897]
HT-29	ED₅₀	>10 μM Compound: scopoletin	Cytotoxicity against human HT-29 cells Cytotoxicity against human HT-29 cells	[PMID: 23301897]
Huh-7	IC₅₀	>25 μM Compound: Scopoletin	Growth inhibition of human HuH7 cells after 48 hrs by MTT assay Growth inhibition of human HuH7 cells after 48 hrs by MTT assay	[PMID: 27956344]
HuT78	IC₅₀	>100 μM Compound: Sco, Scopoletin	Cytotoxicity against human HUT78 cells after 24 hrs by MTT assay Cytotoxicity against human HUT78 cells after 24 hrs by MTT assay	[PMID: 23218780]
HUVEC	ED₅₀	>5 μg/mL Compound: 7-hydroxy-6-methoxycoumarin	Cytotoxicity against HUVEC Cytotoxicity against HUVEC	[PMID: 15043409]
HUVEC	ED₅₀	>5 μg/mL Compound: scopoletin	Cytotoxicity against human HUVEC Cytotoxicity against human HUVEC	[PMID: 15270571]
HUVEC	ED₅₀	>5 μg/mL Compound: scopoletin	Cytotoxicity against HUVEC Cytotoxicity against HUVEC	[PMID: 17190457]
K562	IC₅₀	>100 μM Compound: Sco, Scopoletin	Cytotoxicity against human K562 cells after 24 hrs by MTT assay Cytotoxicity against human K562 cells after 24 hrs by MTT assay	[PMID: 23218780]
KB	ED₅₀	>5 μg/mL Compound: scopoletin	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 15270571]
KB	IC₅₀	4 μg/mL Compound: Scopoltin	Cytotoxicity against human KB cells Cytotoxicity against human KB cells	[PMID: 9214732]
L02	IC₅₀	>25 μM Compound: Scopoletin	Cytotoxicity against human L02 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay Cytotoxicity against human L02 cells assessed as reduction in cell viability measured after 48 hrs by MTT assay	[PMID: 32438251]
L6	IC₅₀	>90 μM Compound: 4	Cytotoxicity against rat L6 cells after 72 hrs by microplate fluorimetry Cytotoxicity against rat L6 cells after 72 hrs by microplate fluorimetry	[PMID: 21438586]
LNCaP	ED₅₀	>5 μg/mL Compound: 7-hydroxy-6-methoxycoumarin	Cytotoxicity against human LNCAP cells Cytotoxicity against human LNCAP cells	[PMID: 15043409]
LNCaP	ED₅₀	>5 μg/mL Compound: scopoletin	Cytotoxicity against human LNCAP cells Cytotoxicity against human LNCAP cells	[PMID: 15270571]
LNCaP	ED₅₀	>5 μg/mL Compound: scopoletin	Cytotoxicity against human LNCAP cells Cytotoxicity against human LNCAP cells	[PMID: 17190457]
LoVo	IC₅₀	18.5 μM Compound: 3	Cytotoxicity against human doxorubicin-resistant LoVo cells after 72 hrs by MTT assay Cytotoxicity against human doxorubicin-resistant LoVo cells after 72 hrs by MTT assay	[PMID: 15387667]
LoVo	IC₅₀	7.3 μM Compound: 3	Cytotoxicity against human LoVo cells after 72 hrs by MTT assay Cytotoxicity against human LoVo cells after 72 hrs by MTT assay	[PMID: 15387667]
Lu1	ED₅₀	>5 μg/mL Compound: 7-hydroxy-6-methoxycoumarin	Cytotoxicity against human Lu1 cells Cytotoxicity against human Lu1 cells	[PMID: 15043409]
Lu1	ED₅₀	>5 μg/mL Compound: scopoletin	Cytotoxicity against human Lu1 cells Cytotoxicity against human Lu1 cells	[PMID: 15270571]
Lu1	ED₅₀	>5 μg/mL Compound: scopoletin	Cytotoxicity against human Lu1 cells Cytotoxicity against human Lu1 cells	[PMID: 17190457]
Macrophage	IC₅₀	5 μM Compound: 4, scopoletin	Antiinflammatory activity in NMRI mouse macrophages assessed as inhibition of A23187-induced PGE2 release by Ellman's method Antiinflammatory activity in NMRI mouse macrophages assessed as inhibition of A23187-induced PGE2 release by Ellman's method	[PMID: 8988605]
Macrophage	IC₅₀	5 μmol Compound: 4, scopoletin	Antiinflammatory activity in NMRI mouse macrophages assessed as inhibition of A23187-induced PGE2 release by Ellman's method Antiinflammatory activity in NMRI mouse macrophages assessed as inhibition of A23187-induced PGE2 release by Ellman's method	[PMID: 8988605]
MCF-10A	IC₅₀	>25 μM Compound: Scopoletin	Cytotoxicity against human MCF-10A cells assessed as reduction in cell viability measured after 48 hrs by MTT assay Cytotoxicity against human MCF-10A cells assessed as reduction in cell viability measured after 48 hrs by MTT assay	[PMID: 34315044]
MCF7	ED₅₀	>5 μg/mL Compound: 7-hydroxy-6-methoxycoumarin	Cytotoxicity against human MCF7 cells Cytotoxicity against human MCF7 cells	[PMID: 15043409]
MCF7	IC₅₀	>50 μM Compound: scopoletin	Cytotoxicity against human MCF7 Cytotoxicity against human MCF7	[PMID: 16038556]
MCF7	ED₅₀	>5 μg/mL Compound: scopoletin	Cytotoxicity against human MCF7 cells Cytotoxicity against human MCF7 cells	[PMID: 17190457]
MCF7	IC₅₀	>100 μM Compound: 7, scopoletin	Antiproliferative activity against human MCF7 cells after 72 hrs by crystal violet staining method Antiproliferative activity against human MCF7 cells after 72 hrs by crystal violet staining method	[PMID: 22765897]
MCF7	IC₅₀	17.11 μg/mL Compound: 14	Cytotoxicity against human MCF7 cells Cytotoxicity against human MCF7 cells	[PMID: 23511021]
MCF7	IC₅₀	>25 μM Compound: Scopoletin	Anticancer activity against human MCF7 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay Anticancer activity against human MCF7 cells assessed as inhibition of cell proliferation measured after 48 hrs by MTT assay	[PMID: 32438251]
MCF7	IC₅₀	>25 μM Compound: Scopoletin	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 34315044]
MCF7	IC₅₀	>80 μM Compound: Scopoletin	Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human MCF7 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 36242987]
MDA-MB-231	IC₅₀	>100 μM Compound: 7, scopoletin	Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by crystal violet staining method Antiproliferative activity against human MDA-MB-231 cells after 72 hrs by crystal violet staining method	[PMID: 22765897]
MDA-MB-231	IC₅₀	>100 μM Compound: Sco, Scopoletin	Cytotoxicity against human MDA-MB-231 cells after 24 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells after 24 hrs by MTT assay	[PMID: 23218780]
MDA-MB-231	IC₅₀	>20 μg/mL Compound: 14	Cytotoxicity against human MDA-MB-231 cells Cytotoxicity against human MDA-MB-231 cells	[PMID: 23511021]
MDA-MB-231	IC₅₀	>30 μM Compound: 6, Scopoletin	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay	[PMID: 25703298]
MDA-MB-231	IC₅₀	>25 μM Compound: Scopoletin	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell growth measured after 48 hrs by MTT assay	[PMID: 34315044]
MDA-MB-435	IC₅₀	>100 μM Compound: Sco, Scopoletin	Cytotoxicity against human MDA-MB-435 cells after 24 hrs by MTT assay Cytotoxicity against human MDA-MB-435 cells after 24 hrs by MTT assay	[PMID: 23218780]
MGC-803	IC₅₀	>80 μM Compound: Scopoletin	Antiproliferative activity against human MGC-803 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human MGC-803 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 36242987]
MKN-45	IC₅₀	>100 μM Compound: Sco, Scopoletin	Cytotoxicity against human MKN45 cells after 24 hrs by MTT assay Cytotoxicity against human MKN45 cells after 24 hrs by MTT assay	[PMID: 23218780]
NCI-H460	IC₅₀	>50 μM Compound: scopoletin	Cytotoxicity against human NCI-H460 Cytotoxicity against human NCI-H460	[PMID: 16038556]
Neutrophil	IC₅₀	>50 μM Compound: 11	Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced elastase release Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced elastase release	[PMID: 18211005]
Neutrophil	IC₅₀	>50 μM Compound: 11	Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced superoxide anion generation Antiinflammatory activity in human neutrophils assessed as inhibition of formyl-L-methionyl-L-leucyl-L-phenylalanine/cytochalasin B-induced superoxide anion generation	[PMID: 18211005]
PANC-1	IC₅₀	>100 μM Compound: Sco, Scopoletin	Cytotoxicity against human PANC1 cells after 24 hrs by MTT assay Cytotoxicity against human PANC1 cells after 24 hrs by MTT assay	[PMID: 23218780]
PBMC	IC₅₀	>100 μM Compound: Sco, Scopoletin	Cytotoxicity against human PBMC after 24 hrs by MTT assay Cytotoxicity against human PBMC after 24 hrs by MTT assay	[PMID: 23218780]
PC-3	IC₅₀	72.19 μM Compound: Scopoletin	Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay Antiproliferative activity against human PC-3 cells assessed as inhibition of cell growth incubated for 48 hrs by CCK-8 assay	[PMID: 36242987]
Platelet	IC₅₀	>50 μg/mL Compound: Scopolatin	Antiaggregatory activity in human platelets assessed as inhibition of thrombin-induced platelet aggregation by aggregometry Antiaggregatory activity in human platelets assessed as inhibition of thrombin-induced platelet aggregation by aggregometry	[PMID: 21106454]
Platelet	IC₅₀	41.74 μg/mL Compound: Scopolatin	Antiaggregatory activity in human platelets assessed as inhibition of collagen-induced platelet aggregation by aggregometry Antiaggregatory activity in human platelets assessed as inhibition of collagen-induced platelet aggregation by aggregometry	[PMID: 21106454]
Sf21	IC₅₀	>100 μM Compound: 7	Inhibition of human recombinant COX2 expressed in insect Sf21 cells by EIA assay Inhibition of human recombinant COX2 expressed in insect Sf21 cells by EIA assay	[PMID: 17378609]
SK-MEL-28	IC₅₀	3.43 μM Compound: 2	Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Anticancer activity against human SK-MEL-28 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37197457]
SK-MEL3	IC₅₀	1.5 μM Compound: 2	Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Anticancer activity against human SK-MEL3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 37197457]
SW-620	IC₅₀	>25 μM Compound: Scopoletin	Growth inhibition of human SW620 cells after 48 hrs by MTT assay Growth inhibition of human SW620 cells after 48 hrs by MTT assay	[PMID: 27956344]
U-937	CC₅₀	600.2 μM Compound: 3	Cytotoxicity against human U937 cells by trypan blue assay after 48 hrs Cytotoxicity against human U937 cells by trypan blue assay after 48 hrs	[PMID: 18060791]
U-937	IC₅₀	151.5 μM Compound: 3	Growth inhibition of human U937 cells by [3H]thymidine incorporation assay Growth inhibition of human U937 cells by [3H]thymidine incorporation assay	[PMID: 18060791]
U-937	IC₅₀	975.9 μM Compound: 8	Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting Antiproliferative activity against human U937 cells assessed as incorporation of [3H]-methyl-thymidine after 12 hrs by scintillation counting	[PMID: 22925447]
U-937	CC₅₀	>2000 μM Compound: 8	Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay Cytotoxicity against human U937 cells after 48 hrs by trypan blue assay	[PMID: 22925447]

In Vitro

Scopoletin (SCT) is identified as a putative inhibitor of acetylcholinesterase (AChE). Scopoletin enhances the K⁺-stimulated release of ACh from rat frontal cortex synaptosomes, showing a bell-shaped dose effect curve (E_max: 4 μM) ^[1]. Scopoletin inhibits PC3 proliferation by inducing apoptosis of PC3 cells. The IC₅₀ of Scopoletin for inhibiting PC3, PAA (human lung cancer cell), and Hela cell proliferation is (157±25), (154±51), and (294±100) mg/L, respectively. Scopoletin induces a marked time- and concentration-dependent inhibition of PC3 cell proliferation. Scopoletin reduces the protein content and decreases the acid phosphatase activity (ACP) level in PC3 cells in a concentration-dependent manner. Cells treated by Scopoletin show typical morphologic changes of apoptosis by light microscope, fluorescence microscope, and transmission electronmicroscope. Apoptosis rate is 0.3 %, 2.1 %, 9.3 % and 35 % for Scopoletin 0, 100, 200, and 400 mg/L, respectively, and cells in G2 phase decrease markedly after being treated with Scopoletin^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Scopoletin (2 μg, i.c.v.) increases T-maze alternation and ameliorated novel object recognition of mice with scopolamine-induced cholinergic deficit. It also reduces age-associated deficits in object memory of 15-18-month-old mice (2 mg/kg sc). Mice injected with 2 μg Scopoletin show an increased alternation rate of 71.3±2.5%^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 92-61-5
Appearance Solid
Molecular Weight 192.17
Formula C₁₀H₈O₄
Color White to yellow
SMILES

O=C1C=CC2=CC(OC)=C(O)C=C2O1
Synonyms

Gelseminic acid; Chrysatropic acid
Structure Classification
- Phenylpropanoids
- Coumarins
- Phenols
- Monophenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (4)

All Apoptosis AnalysisRT-PCRWBELISAIF

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Journal Impact Factor

Phytomedicine

Scopoletin alleviates cognitive deficits in 5xFAD mice via suppressing microglial inflammatory response. [Abstract]

2026 Apr:153:157981. PMID: 41722121

Scopoletin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2026 Apr:153:157981. [Abstract]

qPCR of Il6, Tnf, and Il1b in BV2 cells treated with LPS (100 ng/mL) and Scopoletin (SP, 10–50 μM).

Scopoletin purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2026 Apr:153:157981. [Abstract]

IL-6 and TNF-α in BV2 supernatants by ELISA treated with Scopoletin (SP, 50 μM).
Pestic Biochem Physiol

Mechanistic insights into chlorogenic acid and caffeic acid as novel juvenile hormone antagonists. [Abstract]

2025 Dec:215:106655. PMID: 41162045
Phytopathol Res

2024 Jan 2.
Oxid Med Cell Longev

Morinda citrifolia and Its Active Principle Scopoletin Mitigate Protein Aggregation and Neuronal Apoptosis through Augmenting the DJ-1/Nrf2/ARE Signaling Pathway. [Abstract]

2019 May 2:2019:2761041. PMID: 31191797

Scopoletin purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2019 May 2:2019:2761041. [Abstract]

Rotenone-induced apoptosis is counteracted by Scopoletin (30 μM) in SH-SY5Y cells.

Scopoletin purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2019 May 2:2019:2761041. [Abstract]

Immunoblot analysis of several proteins involved in Nrf2/ARE pathway is performed in SH-SY5Y cells treated with/without Rotenone and Scopoletin (30 μM).

Scopoletin purchased from MedChemExpress. Usage Cited in: Oxid Med Cell Longev. 2019 May 2:2019:2761041. [Abstract]

Iba1 immunofluorescence treated with Scopoletin (SP, 50 μM).

Solvent & Solubility

In Vitro:

DMSO : 62.5 mg/mL (325.23 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Ethanol : < 1 mg/mL (insoluble)

* Scopoletin is usually formulated as a suspension.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.2037 mL

26.0186 mL

52.0373 mL

5 mM

1.0407 mL

5.2037 mL

10.4075 mL

10 mM

0.5204 mL

2.6019 mL

5.2037 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (10.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.08 mg/mL (10.82 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (10.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocol

Cell Assay^[2]

PC3 cells (5×10⁷/L) 1 mL in exponential growth are seeded into four 24-well plates. The plates are incubated at 37°C in a humidified 5% CO₂ atmospbee. After 24h, Scopoletin 33, 66, 133, 266, and 533 mg/L are added to wells (3 wells for each concentration for each plate). For control cells (3 wells foreach plate),only DMEM was added. The plates are incubated continually. The viable cells are counted by hemocytometer every day in the frist 4 d by Trypan blue dye exclusion method^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Administration^[1]

Mice^[1]
C56BL/6N male, 4-6-month-old and 16-18-month-old mice are used in the behavioral studies. The mice are housed in groups of four in cages at constant humidity (50-55%) and temperature (22±1°C) on a 12:12 h light/dark cycle (7:00–19:00 h), with food and water ad libitum. Younger mice (4-6 months) are implanted with i.c.v. cannulas for application of Scopolamine (SCOP) and Scopoletin. The aged mice are injected with Scopoletin by the s.c. route. Experiments are conducted between 8:00 and 16:00 h. Mice with i.c.v. cannulas are randomly divided into four experimental groups: vehicle; SCOP 20 μg; Scopoletin 2 μg; and SCOP 20 μg plus Scopoletin 2 μg. The drugs are applied in 1 μL of vehicle solution (SCOP: saline, Scopoletin: 3 DMSO: 7 sterile water). I.c.v. injections are carried out 15 min before the start of the tests. Aged mice obtained Scopoletin s.c. 30 min prior to object memory test (vehicle: 1 DMSO: 1 EtOH, diluted with olive oil as required)^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (279 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Hornick A, et al. The coumarin Scopoletin potentiates acetylcholine release from synaptosomes, amplifies hippocampal long-term potentiation and ameliorates anticholinergic- and age-impaired memory. Neuroscience. 2011 Dec 1;197:280-92. [Content Brief]

[2]. Liu XL, et al. Effect of Scopoletin on PC3 cell proliferation and apoptosis. Acta Pharmacol Sin. 2001 Oct;22(10):929-33. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.2037 mL	26.0186 mL	52.0373 mL	130.0931 mL
	5 mM	1.0407 mL	5.2037 mL	10.4075 mL	26.0186 mL
	10 mM	0.5204 mL	2.6019 mL	5.2037 mL	13.0093 mL
	15 mM	0.3469 mL	1.7346 mL	3.4692 mL	8.6729 mL
	20 mM	0.2602 mL	1.3009 mL	2.6019 mL	6.5047 mL
	25 mM	0.2081 mL	1.0407 mL	2.0815 mL	5.2037 mL
	30 mM	0.1735 mL	0.8673 mL	1.7346 mL	4.3364 mL
	40 mM	0.1301 mL	0.6505 mL	1.3009 mL	3.2523 mL
	50 mM	0.1041 mL	0.5204 mL	1.0407 mL	2.6019 mL
	60 mM	0.0867 mL	0.4336 mL	0.8673 mL	2.1682 mL
	80 mM	0.0650 mL	0.3252 mL	0.6505 mL	1.6262 mL
	100 mM	0.0520 mL	0.2602 mL	0.5204 mL	1.3009 mL

Keywords

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Scopoletin

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Neuronal Signaling Compound Library

Natural Product Library

Anti-Cancer Compound Library

Medicine Food Homology Compound Library

Phenols Library

Traditional Chinese Medicine Active Compound Library

FDA Approved & Pharmacopeial Drug Library

Anti-Lung Cancer Compound Library

Food-Sourced Compound Library

Anti-Cancer Natural Product Library

Anti-inflammatory Traditional Chinese Medicine Active Compound Library

Plant-Sourced Natural Product Library

Human Metabolite Library

Membrane Protein-targeted Compound Library

Cell Death Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Miao Ethnicity Medicine Compound Library

Tibetan Medicine Compound Library

PBCRBS Traditional Chinese Medicine Compound Library

Heat-Clearing and Detoxification Traditional Chinese Medicine Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

RO5 Drug-like Natural Product Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Ancient Chinese Classical Formulas Traditional Chinese Medicine Active Compound Library

Mass Spectrometry Human Metabolite Library

Antitussive and Antiasthmatic Traditional Chinese Medicine Active Compound Library

Diarrhea-Related Traditional Chinese Medicine Active Compound Library

Mongolian Medicine Compound Library

Chinese Baijiu Compound Library

Tonifying Traditional Chinese Medicine Monomer Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Scopoletin

All Cholinesterase (ChE) Isoforms

Biological Activity

Chemical Information

Publications (4)

Solvent & Solubility

Protocol

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Scopoletin Related Classifications

Cat. No.: HY-N0342