TB500 

TB500 is a synthetic version of an active region of thymosin β₄. TB500 exhibits anti-fibrotic and wound healing activities by inhibiting the Akt signaling pathway and binding to actin. TB500 is claimed to promote endothelial cell differentiation, angiogenesis in dermal tissues, keratinocyte migration, collagen deposition and decrease inflammation^[1][2][3].

TB500 exhibits tissue repairing and regenerative activities through its active ingredient Ac-Tβ4_17-23. Ac-Tβ4_17-23 prevents apoptosis, promotes cell survival and angiogenesis, stimulates T cell proliferation, exhibiting immunomodulatory activity^[2].
TB500 (15-25 μg/mL, 2 days) promotes cell migration and wound healing by enhancing ATP-induced increase in intracellular calcium ions and activating purinergic P2 receptors^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

TB500 (10 mg/dose, single dose) can be used as doping agent in horse and greyhound racing^[2].
TB500 (15 μg/30 μL, topical administration, once daily for 10 days) promotes wound healing, and exhibits good tissue repair effects in BALB/c mouse models^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

889.01

C₃₈H₆₈N₁₀O₁₄

885340-08-9

Solid

White to off-white

N-Acetyl-Leu-Lys-Lys-Thr-Glu-Thr-Gln

Ac-LKKTETQ

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture and light, under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. Emmie N M Ho, et al. Doping control analysis of TB-500, a synthetic version of an active region of thymosin β₄, in equine urine and plasma by liquid chromatography-mass spectrometry. J Chromatogr A. 2012 Nov 23;1265:57-69.  [Content Brief]

  [2]. Esposito S, et al., Synthesis and characterization of the N-terminal acetylated 17-23 fragment of thymosin beta 4 identified in TB-500, a product suspected to possess doping potential. Drug Test Anal. 2012 Sep;4(9):733-8.  [Content Brief]

  [3]. Huang CM, et al., In vivo detection of secreted proteins from wounded skin using capillary ultrafiltration probes and mass spectrometric proteomics. Proteomics. 2006 Nov;6(21):5805-14.  [Content Brief]

  [4]. Shah R, et al., Thymosin β4 inhibits PDGF-BB induced activation, proliferation, and migration of human hepatic stellate cells via its actin-binding domain. Expert Opin Biol Ther. 2018 Jul;18(sup1):177-184.  [Content Brief]