Tanshinone I
Based on 5 publication(s) in Google Scholar
Tanshinone I is an inhibitor of type IIA human recombinant sPLA2 (IC50=11 μM) and rabbit recombinant cPLA2 (IC50=82 μM).
For research use only. We do not sell to patients.
- Purity: 98.83%
- CAS No.: 568-73-0
- Formula: C18H12O3
- Molecular Weight:276.29
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) Tanshinone I
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Customer Validation & Images
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Cell Proliferation/Viability Assay
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Flow Cytometry
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RT-PCR
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WB
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Cell Imaging/Staining
All Phospholipase Isoforms
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Biological Activity
IC50: 11 μM (sPLA2), 82 μM (cPLA2)[1].
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| AGS | IC50 |
>50 μM
Compound: 6, tanshinone i
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Viability of human AGS cells under normoxic conditions after 24 hrs by MTT assay
Viability of human AGS cells under normoxic conditions after 24 hrs by MTT assay
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[PMID: 17583950] |
| AGS | IC50 |
12.5 μM
Compound: 6, tanshinone i
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Inhibition of HIF1 activation in human AGS cells assessed as inhibition of hypoxia-induced luciferase expression after 16 hrs by reporter assay
Inhibition of HIF1 activation in human AGS cells assessed as inhibition of hypoxia-induced luciferase expression after 16 hrs by reporter assay
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[PMID: 17583950] |
| Hep 3B2 | EC50 |
>10 μM
Compound: 3
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Cytotoxicity against human Hep3B cells deficient in p53 gene by MTT assay
Cytotoxicity against human Hep3B cells deficient in p53 gene by MTT assay
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[PMID: 20455578] |
| Hep 3B2 | IC50 |
>50 μM
Compound: 6, tanshinone i
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Viability of human Hep3B cells under normoxic conditions after 24 hrs by MTT assay
Viability of human Hep3B cells under normoxic conditions after 24 hrs by MTT assay
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[PMID: 17583950] |
| Hep 3B2 | IC50 |
8.51 μM
Compound: 6, tanshinone i
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Inhibition of HIF1 activation in human Hep3B cells assessed as inhibition of hypoxia-induced luciferase expression after 16 hrs by reporter assay
Inhibition of HIF1 activation in human Hep3B cells assessed as inhibition of hypoxia-induced luciferase expression after 16 hrs by reporter assay
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[PMID: 17583950] |
| HepG2 | EC50 |
>10 μM
Compound: 3
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Cytotoxicity against doxorubicin resistant human HepG2 cells expressing wild type p53 gene by MTT assay
Cytotoxicity against doxorubicin resistant human HepG2 cells expressing wild type p53 gene by MTT assay
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[PMID: 20455578] |
| HepG2 | EC50 |
6.3 μM
Compound: 3
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Cytotoxicity against human HepG2 cells expressing wild type p53 gene by MTT assay
Cytotoxicity against human HepG2 cells expressing wild type p53 gene by MTT assay
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[PMID: 20455578] |
| KB | IC50 |
5.87 μM
Compound: 1
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Antiproliferative activity against human KB cells after 72 hrs by sulforhodamine B assay
Antiproliferative activity against human KB cells after 72 hrs by sulforhodamine B assay
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[PMID: 29294282] |
| MCF7 | ED50 |
0.82 μg/mL
Compound: Tanshinone I
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Dose required for reduction in cell growth of human breast cancer MCF-7 cell line
Dose required for reduction in cell growth of human breast cancer MCF-7 cell line
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[PMID: 15509181] |
| MCF7 | IC50 |
7.927 μM
Compound: 1
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Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 24 hrs by MTT assay
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability after 24 hrs by MTT assay
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[PMID: 33751939] |
| MDA-MB-231 | ED50 |
0.75 μg/mL
Compound: Tanshinone I
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Dose required for reduction in cell growth of human breast cancer MDA-MB-231 cell line
Dose required for reduction in cell growth of human breast cancer MDA-MB-231 cell line
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[PMID: 15509181] |
| MDA-MB-231 | IC50 |
7.962 μM
Compound: 1
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Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 24 hrs by MTT assay
Cytotoxicity against human MDA-MB-231 cells assessed as reduction in cell viability after 24 hrs by MTT assay
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[PMID: 33751939] |
| MIA PaCa-2 | IC50 |
10.5 μM
Compound: 15
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Cytotoxicity against human MIAPaCa2 cells after 24 hrs by MTT assay
Cytotoxicity against human MIAPaCa2 cells after 24 hrs by MTT assay
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[PMID: 21775156] |
| PLC-PRF-5 | EC50 |
>10 μM
Compound: 3
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Cytotoxicity against human PLC/PRF/5 cells expressing mutant p53 gene (codon 249) by MTT assay
Cytotoxicity against human PLC/PRF/5 cells expressing mutant p53 gene (codon 249) by MTT assay
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[PMID: 20455578] |
| Vero C1008 | CC50 |
>200 μM
Compound: 29
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Cytotoxicity against African green monkey Vero E6 cells assessed as cell viability treated for 24 hrs by CCK8 assay
Cytotoxicity against African green monkey Vero E6 cells assessed as cell viability treated for 24 hrs by CCK8 assay
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[PMID: 35620927] |
Tanshinone I inhibits PGE2 formation from LPS-induced RAW macrophages (IC50=38 μM). When Tanshinone I is added simultaneously with LPS, this compound clearly inhibits PGE2 production (IC50=38 μM) at 10-100 μM. Tanshinone I also reduces PGE2 production (IC50=46 μM) when added after COX-2 is fully induced. The fact that Tanshinone I inhibits PGE2 production by pre-induced COX-2 strongly suggests that this compound may directly inhibit COX-2 activity and/or affect PLA2 activity. When Tanshinone I is incubated with two different forms of phospholipase A2 (PLA2), it clearly inhibits sPLA2 (IC50=11 μM) in a concentration-dependent manner. Although being less potent, Tanshinone I also inhibits cPLA2 (IC50=82 μM)[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 568-73-0
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Appearance Solid
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Molecular Weight 276.29
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Formula C18H12O3
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Color Light brown to black
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SMILES
O=C(C1=C2C=CC3=C1C=CC=C3C)C(C4=C2OC=C4C)=O
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Synonyms
Tanshinone A
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (5)
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Journal Impact Factor
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Most Recent
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Int J Mol Sci
PLA2G2A Phospholipase Promotes Fatty Acid Synthesis and Energy Metabolism in Pancreatic Cancer Cells with K-ras Mutation. [Abstract]2022 Oct 3;23(19):11721. PMID: 36233022
Tanshinone I purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 3;23(19):11721. [Abstract]
PANC-1 and AsPC-1 cells were treated with various concentrations (0.5–5 µM) of Tanshinone I for various time periods, as indicated. Cell proliferation was analyzed by MTT assay.
Tanshinone I purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 3;23(19):11721. [Abstract]
PANC-1 and AsPC-1 cells were incubated with 5 µM Tanshinone I for 72 h; lipid peroxidation was analyzed by flow cytometry.
Tanshinone I purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 3;23(19):11721. [Abstract]
PANC-1 cells were incubated with Tanshinone I (5–10 µM) for 72 h. The mRNA levels of fatty acid synthesis-related genes were quantified by qRT-PCR.
Tanshinone I purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 3;23(19):11721. [Abstract]
AsPC-1 cells were incubated with Tanshinone I (1–10 µM) for 72 h. FASN protein level was analyzed by western blot analysis.
Tanshinone I purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2022 Oct 3;23(19):11721. [Abstract]
PANC-1 and AsPC-1 cells were treated with 5 µM Tanshinone I and incubated for two weeks to form colonies.
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Fish Shellfish Immunol
Emodin enhances host antiviral immunity against Micropterus salmoides rhabdovirus by activating RLR signaling in largemouth bass. [Abstract]2025 Aug 6:166:110633. PMID: 40769268 -
Mol Immunol
Tanshinone I limits inflammasome activation of macrophage via docking into Syk to alleviate DSS-induced colitis in mice. [Abstract]2024 Jul 31:173:88-98. PMID: 39088935 -
Life Sci Alliance
Tanshinone functions as a coenzyme that confers gain of function of NQO1 to suppress ferroptosis. [Abstract]2022 Nov 1;6(1):e202201667. PMID: 36319062 -
Solvent & Solubility
DMSO : 2 mg/mL (7.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
As sources of PLA2, human recombinant sPLA2 (type IIA) is purified from CHO cells transfected with the PLA2 gene and rabbit recombinant platelet cPLA2 is obtained through its expression in baculovirus. The standard reaction mixture (200 μL) contained 100 mM Tris-HCl buffer (pH 9.0) with 6 mM CaCl2 and 20 nmol 1-acyl-[1-14C]-arachidonyl-sn-glycerophosphoethanolamine (2000 cpm/nmol) in the presence or absence of Tanshinone I. The reaction is started by adding 50 ng purified sPLA2 or cPLA2. After 20 min at 37°C, the free fatty acid generated is analysed. Under these standard conditions, the reaction mixture in the absence of Tanshinone I released approximately 10% of free fatty acid from the phospholipid substrate added[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
RAW 264.7 cells are cultured with DMEM supplemented with 10% FBS and 1% antibiotics under 5% CO2 at 37°C. Briefly, cells are plated in 96-well plates (2×105 cells/well). LPS (1 ug/mL) and Tanshinone I are simultaneously added and incubated for 24 h, unless otherwise specified. The PGE2 concentration in the medium is measured using an EIA kit for PGE2. In order to determine the effects of Tanshinone I on PGE2 production after induction of COX-2, cells are incubated with LPS (1 ug/mL) for 24 h and thoroughly washed. Then, Tanshinone I is added without LPS and the cells are incubated for another 24 h. From the medium, PGE2 concentrations are measured. The cytotoxicity of Tanshinone I to RAW cells is checked using the MTT assay. Tanshinone I does not show any cytotoxicity up to 100 uM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Mice[1]
In order to evaluate the inhibitory activity of Tanshinone I against animal models of acute and chronic inflammation, rat carrageenan (CGN)-induced paw oedema and adjuvant-induced arthritis (AIA) models are employed. Briefly, 1% CGN dissolved in pyrogen-free saline (0.05 mL) is injected into right hind paw of rats for the paw oedema test. After 5 h, swelling of the treated paw is measured using a plethysmometer. Tanshinone I dissolved in 0.5% CMC is administered orally 1 h prior to CGN injection. For the AIA test, an arthritic inflammation is provoked by injection of Mycobacterium Butyricum (0.6 mL/rat) dissolved in mineral oil to the right hind paw of rats. Tanshinone I is orally administered every day. The swelling of the treated and untreated paws is measured using a plethysmometer.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (285 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.6194 mL | 18.0969 mL | 36.1939 mL | 90.4846 mL |
| 5 mM | 0.7239 mL | 3.6194 mL | 7.2388 mL | 18.0969 mL |
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