2. Signaling Pathways
  3. Metabolic Enzyme/Protease
  4. Dihydrofolate reductase (DHFR)

Dihydrofolate reductase (DHFR)

Dihydrofolate reductase (DHFR) catalyzes the reduction of dihydrofolate to tetrahydrofolate using NADPH, and it is involved in the synthesis of nucleotide, in both prokaryotic and eukaryotic cells. DHFR inhibitors are commonly used for fighting malaria and other protozoal infections, as well as for treating fungal, bacterial, and mycobacterial infections.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-181813
    PROTAC DHFR Degrader-1
    		Degrader
    PROTAC DHFR Degrader-1 is a selective PROTAC degrader targeting Plasmodium falciparum DHFR-TS with a Ki of 2.01 nM. PROTAC DHFR Degrader-1 exhibits no inhibitory activity against human DHFR and suppresses the growth of Plasmodium falciparum. PROTAC DHFR Degrader-1 can be used for the research of Plasmodium falciparum and malaria.
    PROTAC DHFR Degrader-1
  • HY-119613
    Clociguanil
    		Inhibitor
    Clociguanil (BRL 50216), a DHFR inhibitor (IC50: 55.6 nM), is an antimalarial agent. Clociguanil has sympathomimetic and antiarrhythmic action.
    Clociguanil
  • HY-161292
    DHFR-IN-16
    		Inhibitor
    DHFR-IN-16 (compound 8d) is an inhibitor of dihydrofolate reductase (DHFR) (IC50=0.199 μM) and can be used in anti-infectious research.
    DHFR-IN-16
  • HY-118251
    PD130883
    		Inhibitor
    PD130883 is an antifolate DHFR inhibitor that directly inhibits thymidylate synthase at the 5,10-methylenetetrahydrofolate binding site, reducing the net conversion of tetrahydrofolate cofactor to dihydrofolate.
    PD130883
  • HY-169211
    DHFR-IN-21
    DHFR-IN-21 (compund 5p) is a dihydrofolate reductase (DHFR) inhibitor.
    DHFR-IN-21
  • HY-156414
    DHFR-IN-11
    		Inhibitor
    DHFR-IN-11 (compound 6b) is inhibitor of DHFR, and has inhibitory potency versus M. tuberculosis DHFR enzyme with IC50 values 5.70 μM.
    DHFR-IN-11
  • HY-161063
    DHFR-IN-14
    		Inhibitor
    DHFR-IN-14 (compound 32) is a pyrimethamine (Pyr)-type, dihydrofolate reductase (DHFR) inhibitor with potential anticancer activity.
    DHFR-IN-14
  • HY-163725
    DHFR-IN-18
    		Inhibitor
    DHFR-IN-18 (compound 19D) is a DHFR inhibitor. DHFR-IN-18 can be used in anti-leukemia research.
    DHFR-IN-18
  • HY-105714
    Etoprine
    		Inhibitor
    Etoprine is a potent orally active dihydrofolate reductase (DHFR) inhibitor. Etoprine competitively inhibits testicular DHFR to disrupt tetrahydrofolate and DNA synthesis in rapidly dividing cells. Etoprine acts as an antifertility agent in male mice and reduces fecundity in male rats.
    Etoprine
  • HY-181100
    DHFR-IN-26
    		Inhibitor
    DHFR-IN-26 is an Escherichia coli dihydrofolate reductase (ecDHFR) inhibitor with an IC50 of 0.75 nM. DHFR-IN-26 exerts broad-spectrum antibacterial activity. DHFR-IN-26 disrupts folate metabolism, nucleotide synthesis, and bacterial amino acid metabolic pathways. DHFR-IN-26 disrupts bacterial inner membranes, inhibited biofilm formation, and attenuated phage-related processes. DHFR-IN-26 shows lower toxicity to non-cancerous cells. DHFR-IN-26 can be used for the research of bacterial infections (including infections caused by methicillin-resistant Staphylococcus aureus, multidrug-resistant Escherichia coli, multidrug-resistant Pseudomonas aeruginosa, and lysogenic bacteria).
    DHFR-IN-26
  • HY-179415
    DHFR-IN-24
    		Inhibitor
    DHFR-IN-24, a benzothiazole derivative, is a dihydrofolate reductase (DHFR) inhibitor. DHFR-IN-24 has intrinsic antibacterial activity against both Gram-positive and Gram-negative strains. DHFR-IN-24 synergistically combines DHFR inhibition with photodynamic therapy (PDT) for enhanced antibacterial activity against multidrug-resistant pathogens.
    DHFR-IN-24
  • HY-149699
    DHFR-IN-12
    		Inhibitor
    DHFR-IN-12 (Compound 5c) is a DHFR inhibitor which has good antibacterial activity.
    DHFR-IN-12
  • HY-10373R
    Trimetrexate (Standard)
    		Inhibitor
    Trimetrexate (Standard) is the analytical standard of Trimetrexate (HY-10373). This product is intended for research and analytical applications. Trimetrexate (CI-898) is an antibiotic, also a potent and orally active dihydrofolate reductase (DHFR) inhibitor, reducing the production of DNA and RNA precursors and leading to cell death, with IC50 values of 4.74 nM and 1.35 nM for human DHFR and Toxoplasma gondii DHFR. Trimetrexate can also inhibit the growth of various cancer cells. Trimetrexate can be used for researching Pneumocystis carinii pneumonia (PCP) and cancer.
    Trimetrexate (Standard)
  • HY-153007A
    DHFR-IN-5 hydrochloride
    		Inhibitor
    DHFR-IN-5 hydrochloride is a potent and orally active dihydrofolate reductase (DHFR) inhibitor with a Ki value of 0.54 nM for quadruple mutant Plasmodium falciparum DHFR. DHFR-IN-5 hydrochloride shows anti-malaria activity.
    DHFR-IN-5 hydrochloride
  • HY-B0921R
    Succinylsulfathiazole (Standard)
    		Inhibitor
    Succinylsulfathiazole (Standard) (Succinylsulphathiazole) is the analytical standard of Succinylsulfathiazole (HY-B0921). This product is intended for research and analytical applications. Succinylsulfathiazole is a long-acting sulfonamide antibiotic with localized gut-specific antibacterial activity and is orally active. Succinylsulfathiazole inhibits bacterial folate synthesis, reduces coliform counts, suppresses intestinal bacterial growth and vitamin biosynthesis, and depletes gut folate-producing bacteria. Succinylsulfathiazole modulates hepatic mTOR signaling, diminishes cecal fermentation, decreases hepatic folate levels and total folate excretion, elevates nitrogen excretion and reduces the fermentability of certain dietary fibers. Succinylsulfathiazole induces folate deficiency and triggers biotin- and folate-related nutritional deficiency symptoms in rats and C57BL/6 mice.
    Succinylsulfathiazole (Standard)
  • HY-N3764
    Diosbulbin C
    		Activator
    Diosbulbin C is a diterpene lactone component, which can be extracted from traditional Chinese medicine Dioscorea bulbifera L.. Diosbulbin C possesses high anticancer activity in non-small cell lung cancer (NSCLC). Diosbulbin C could induce cell cycle arrest at G0/G1 phase in NSCLC. Diosbulbin C also inhibits the proliferation of NSCLC cells, possibly by downregulating the expression/activation of AKT, DHFR, and TYMS.
    Diosbulbin C
  • HY-162656
    DHFR-IN-19
    		Inhibitor
    DHFR-IN-19 (Compound 2g) is a selective inhibitor for TbDHFR with Ki of 9 nM. DHFR-IN-19 exhibits antiparasitic activity against Trypanosoma brucei with an EC50 of 14.5 μM.
    DHFR-IN-19
  • HY-115836
    DDPO
    		Inhibitor
    DDPO is an inhibitor for dihydrofolate reductase (DHFR) with an IC50 of 0.035 µM. DDPO inhibits the cell growth of cancer cell L1210 and WI-L2with IC50 of 5 and 0.28 µM.
    DDPO
Cat. No. Product Name / Synonyms Application Reactivity