2. Signaling Pathways
  3. Others
  4. Fluorescent Dye

Fluorescent Dye

Fluorescence is a phenomenon of photoluminescence in luminescence. When an atom is irradiated by light, some electrons around the nucleus jump from their original orbitals to higher energy orbitals under the influence of light energy, i.e., from the ground state to the first or second excited single-line state, etc. Due to the instability of the first or second excited singlet state, the electron returns from the excited singlet state to the ground state accompanied by released energy, resulting in fluorescence.

Fluorescent dyes can be covalently bound or physically adsorbed in the structure of the substance. The fluorescence properties allow for the characterization, localization and quantitative analysis of the labeled object. Fluorescent dyes have shown great potential for the detection of proteins, nucleic acids, cells, and immunoassays, attributed to the advantages in non-radioactive contamination, simple experimental operation and easy observation.

Fluorescent dyes have penetrated many fields such as pharmacology, physiology, environmental science, and information science, as well as functional studies of proteins and drug screening.

Cat. No. Product Name Effect Purity Chemical Structure
  • HY-D2299
    AIE-ER
    		98.55%
    AIE-ER is a specific endoplasmic reticulum (ER) fluorescent probe (green channel: λex=405 nm, λem=450~650 nm) that exhibits remarkable photostability, high brightness, and low working concentration. AIE-ER may provide an avenue for studying diseases related to the endoplasmic reticulum.
    AIE-ER
  • HY-13443F2
    Exendin-4, Cy5 labeled
    Exendin-4, Cy5-labeled (Exendin-4-Cys(Cy5)) is a covalently linked Cy5 fluorescent group to Exendin-4 (HY-13443), a GLP-1 receptor agonist. Exendin-4, Cy5-labeled enables the visualization imaging of β cells in vivo, especially for evaluating the expression dynamics of GLP-1R in type 2 diabetes models.
    Exendin-4, Cy5 labeled
  • HY-126172
    9-Anthryldiazomethane
    9-Anthryldiazomethane is a fluorescent labeling reagent, which can be used for detecting fatty acids and derivatives.
    9-Anthryldiazomethane
  • HY-NP0159
    Wheat Germ Agglutinin (Rhodamine)
    Wheat Germ Agglutinin (WGA) Rhodamine is a plant lectin that can be used as a probe to specifically bind biomolecules (such as polysaccharides, peptides, etc.).Wheat Germ Agglutinin (WGA) Rhodamine is a biological material or organic compound that can be used in life science research.
    Wheat Germ Agglutinin (Rhodamine)
  • HY-D2335
    SERTlight
    		99.73%
    SERTlight is a fluorescent agent that can specifically label serotonergic neuronal cell bodies, dendrites, and axonal projections as fluorescent substrates of the serotonin transporter (SERT). SERTlight is orthogonal to various genetically encoded sensors in terms of optics, pharmacology, and operation, enabling multiple imaging. SERTlight allows the use of GRAB5HT sensors to label distal 5HT axonal projections and simultaneously image the release of endogenous 5HT, providing a new multifunctional molecular tool for studying serotonergic systems.
    SERTlight
  • HY-P2208
    Z-IETD-AFC
    		99.74%
    Z-IETD-AFC, a specific fluorescence substrate, can be used to determine the caspase-8 catalytic activity.
    Z-IETD-AFC
  • HY-D2794
    FITC-PEG400-NH2
    FITC-PEG400-NH2 is a fluorescent dye composed of polyethylene glycol (PEG), FITC (HY-66019) and an amino group. FITC-PEG400-NH2 can be used for fluorescent labeling and imaging.
    FITC-PEG400-NH2
  • HY-153081
    eGFP circRNA
    		98.3%
    The eGFP circRNA will express green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. The eGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence with an emission peak at 509 nm. The eGFP circRNA is therefore ideal for monitoring and optimizing transfection efficiency and is recommended as a positive control for circular RNA transfections.
    eGFP circRNA
  • HY-137896
    4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid disodium
    4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid disodium is a fluorescent dye. 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid disodium can be used to demonstrate retrograde axonal transport to label secondary antibodies and as a fluorescent whole cell stain.
    4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid disodium
  • HY-D2873
    Sulfo Cy7 tyramide
    		Chemical 99.13%
    Sulfo Cy7 tyramide, a red fluorescent dye (Ex=740 nm, Em=770 nm), is utilized as reporter fluorescent substrate of horseradish peroxidase (HRP)-catalyzed deposition for tyramide signal amplification (TSA). Sulfo Cy7 tyramide can be used for multiplex Immunohistochemistry (mIHC).
    Sulfo Cy7 tyramide
  • HY-D1225
    Reactive red 180
    Reactive red 180 is common textile dyes that can be adsorbed onto single-walled carbon nanotubes (SWCNTs) through electrostatic interactions, allowing the separation of residual dyes.
    Reactive red 180
  • HY-D0114
    3-Carboxy-7-Hydroxycoumarin
    		99.10%
    3-Carboxy-7-Hydroxycoumarin is a UV-light excitable fluorescent dye.
    3-Carboxy-7-Hydroxycoumarin
  • HY-D0121A
    INDO 1 pentapotassium
    INDO 1 pentapotassium is a cell-impermeant fluorescent Ca2+ indicator, binds specifically to Ca2+ while emitting fluorescence, the maximum emission wavelength shifts from 485 nm before binding to 410 nm.
    INDO 1 pentapotassium
  • HY-D2166
    AF 594 NHS ester
    AF 594 NHS ester is a derivative of the red fluorescent dye AF 594 with high fluorescence quantum yield and high photostability (Ex=594 nm, Em=615 nm). AF 594 NHS ester can form an ester bond by reacting the NHS group with ammonia, with maximum excitation wavelength of 594 nm.
    AF 594 NHS ester
  • HY-D0214A
    Acid Red 94 lactone
    		99.3%
    Acid Red 94 lactone is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells.
    Acid Red 94 lactone
  • HY-D1436
    PBFI
    PBFI is the cell-impermeant fluorescent indicator that is widely used for the determination of concentrations of K+ (Ex/Em = 340,380/500 nm).
    PBFI
  • HY-DY1063
    CY5-SE (solution)
    Cy5-SE (Cy5 NHS Ester) (solution) is a reactive dye for the labeling of amino-groups in peptides, proteins, and oligonucleotides. This dye requires small amount of organic co-solvent (such as DMF or DMSO) to be used in labeling reaction. This reagent is ideal for very cost-efficient labeling of soluble proteins, as well as all kinds of peptides and oligonucleotides. This reagent also works well in organic solvents for small molecule labeling. Excitation (nm) :649, Emission (nm) : 670.
    Solvent and concentration: DMSO: 10 mM
    CY5-SE (solution)
  • HY-131020
    Janelia Fluor 525, SE
    Janelia Fluor 525, SE (JF525, SE) (the SE form of compound 12) is a yellow fluorescent dye (Ex = 525 nm; Em = 549 nm). Janelia Fluor 525, SE contains an NHS ester, which can be used to label primary amines and is suitable for live cell imaging.
    Janelia Fluor 525, SE
  • HY-D1401
    OG 488, acid
    		99.72%
    OG 488, acid, a fluorescent pH indicator, has many applications in biochemistry and neurosciences.
    OG 488, acid
  • HY-DY1009
    CFDA-SE (solution)
    CFDA-SE (solution) is a fluorescent dye that can penetrate the cell membrane. It can react with the free amine group in the cytoskeleton protein inside the cell, and finally form a protein complex with fluorescence. After entering the cell, CFDA-SE locates in the cell membrane, cytoplasm and nucleus, and the fluorescence staining is strongest in the nucleus. CFDA-SE dye can be uniformly inherited by the cells with cell division and proliferation, and its attenuation is proportional to the number of cell divisions. This phenomenon can be detected and analyzed by flow cytometry under the excitation light of 488 nm, and can be used to detect the proliferation of cells.
    Solvent and concentration: DMSO: 5 mM
    CFDA-SE (solution)
Cat. No. Product Name / Synonyms Application Reactivity