Sulfo Cy7 tyramide

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Sulfo Cy7 tyramide assay
a. Cells are fixed with 3.7% paraformaldehyde in 1x PBS at RT for 20 minutes, and then rinse with 1x PBS twice.
b. permeabilize in 0.1% Triton X-100 for 1-5 minutes at RT, and then rinse with 1x PBS twice.
c. Incubate in 3% H₂O₂ in 1x PBS for 10 min to block endogenous peroxidase activity, and then rinse with 1x PBS twice.
d. Incubate in 1x PBS plus 1% BSA for 30 min at 4°C.
e. Apply the primary antibodies working solution for 1 h at RT or overnight at 4 °C, and then rinse with 1x PBS three times, 5 minutes each.
f. Apply 100 μL secondary antibody-HRP working solution for 1 h at RT and then rinse with 1x PBS three times, 5 minutes each.
g. Incubate in 100 μL Sulfo Cy7 tyramide working solution for 5-10 minutes at RT, and then rinse with 1x PBS three times, 5 minutes each.
h. Fluorescence microscopy detection.
Note: it is suggested that the optimal concentration of Sulfo Cy7 tyramide working solution according to actual conditions.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.