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Cat. No.: HY-D0114

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3-Carboxy-7-Hydroxycoumarin

Name: 3-Carboxy-7-Hydroxycoumarin
Brand: MedChemExpress
SKU: HY-D0114
Price: 25.00 USD
Availability: InStock
Rating: 5 (1 reviews)

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3-Carboxy-7-Hydroxycoumarin is a 7-hydroxycoumarin (umbelliferone) fluorescent dye and antibody-conjugated labeling agent with UV excitation properties. 3-Carboxy-7-Hydroxycoumarin undergoes deprotonation in neutral-to-alkaline environments, producing strong blue fluorescence. The absorption/emission maxima of 3-Carboxy-7-Hydroxycoumarin are 386/448 nm. 3-Carboxy-7-Hydroxycoumarin is applicable for related research such as fluorescent probe synthesis and covalent protein labeling.

For research use only. We do not sell to patients.

Purity : 99.10%
CAS No.: 779-27-1
Formula: C₁₀H₆O₅
Molecular Weight:206.15

Storage:

4°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

3-Carboxy-7-Hydroxycoumarin Chemical Structure

CAS No.: 779-27-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
- Emission (Em)
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

Cellular Effect

Cell Line	Type	Value	Description	References
A 172	IC₅₀	>200 μM Compound: 4	Cytotoxicity against human A172 cells by MTT assay Cytotoxicity against human A172 cells by MTT assay	[PMID: 25304898]
A549	IC₅₀	>200 μM Compound: 4	Cytotoxicity against human A549 cells by MTT assay Cytotoxicity against human A549 cells by MTT assay	[PMID: 25304898]
Bel-7402	IC₅₀	>200 μM Compound: 4	Cytotoxicity against human Bel7402 cells by MTT assay Cytotoxicity against human Bel7402 cells by MTT assay	[PMID: 25304898]
HepG2	IC₅₀	>200 μM Compound: 4	Cytotoxicity against human HepG2 cells by MTT assay Cytotoxicity against human HepG2 cells by MTT assay	[PMID: 25304898]
K562	IC₅₀	>200 μM Compound: 4	Cytotoxicity against human K562 cells by MTT assay Cytotoxicity against human K562 cells by MTT assay	[PMID: 25304898]

In Vitro

3-Carboxy-7-Hydroxycoumarin can be chemically activated to generate NHS ester derivatives for covalent bioconjugation with amino-containing biomacromolecules^[1].

3-Carboxy-7-Hydroxycoumarin (compound 2) exhibits a pK_a of 7.5, absorption/emission maxima at 386/448 nm, an extinction coefficient of 36.7 × 10³, and a quantum yield of 0.70 in 0.1 M phosphate buffer at pH 10^[1]^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

3‑Carboxy‑7‑Hydroxycoumarin‑derived chlorinated analogue V450 is suitable for multicolor immunophenotyping panels and yields bright, well‑separated cell populations^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emission (Em)

454

Chemical Information

CAS No. 779-27-1
Appearance Solid
Molecular Weight 206.15
Formula C₁₀H₆O₅
Color Off-white to yellow
SMILES

O=C(C1=CC2=CC=C(O)C=C2OC1=O)O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light, stored under nitrogen

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Abrams B, et al. 3-Carboxy-6-chloro-7-hydroxycoumarin: a highly fluorescent, water-soluble violet-excitable dye for cell analysis. Analytical biochemistry. 2009 Mar 15;386(2):262-9.

[2]. Sun WC, et al. Synthesis of novel fluorinated coumarins: excellent UV-light excitable fluorescent dyes. Bioorganic & medicinal chemistry letters. 1998 Nov 17;8(22):3107-10. [Content Brief]

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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