2. NF-κB Metabolic Enzyme/Protease Immunology/Inflammation Autophagy
  3. Reactive Oxygen Species (ROS) Autophagy
  4. Tempol

Tempol  (Synonyms: 4-Hydroxy-TEMPO; 1-Oxyl-2,2,6,6-tetramethyl-4-hydroxypiperidine; 4-ヒドロキシ-2,2,6,6-テトラメチルピペリジン1-オキシル; 4-ヒドロキシ-TEMPO)

製品番号: HY-100561 純度: 99.73%
COA 取扱説明書 Technical Support

Tempol is a general superoxide dismutase (SOD)-mimetic agent that efficiently neutralizes reactive oxygen species (ROS).

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CAS 番号 : 2226-96-2

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water
ready for reconstitution
 USD 33 在庫あり
Solution
10 mM * 1 mL in Water USD 33 在庫あり
Solid
200 mg $30 在庫あり
1 g $50 在庫あり
5 g $125 在庫あり
10 g $188 在庫あり
25 g $320 在庫あり
50 g   お問い合わせ  

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カスタマーレビュー

Based on 28 publication(s) in Google Scholar

Other Forms of Tempol:

Tempol 関連抗体

Top Publications Citing Use of Products
Bio/Physico-chemical Assay
Cell Proliferation/Viability Assay
Cell Migration/Invasion Assay
Cell Imaging/Staining
WB

    Tempol purchased from MedChemExpress. Usage Cited in: Exp Mol Med. 2025 Mar;57(3):567-583.  [Abstract]

    The summary data for the PYRVLO spikes evoked by step depolarizing currents during the preapplication of Tempol (1 mM) and coapplication of Tempol and PACs, n = 7.

    Tempol purchased from MedChemExpress. Usage Cited in: Redox Rep. 2025 Dec;30(1):2494314.  [Abstract]

    Tempol (1 mM; 1 h) inhibited Norepinephrine (NE) (20 μM; 1 h)-induced vascular adventitial fibroblast (VAF) migration in WKY and SHR rats.

    Tempol purchased from MedChemExpress. Usage Cited in: Redox Rep. 2025 Dec;30(1):2494314.  [Abstract]

    Tempol (1 mM; 1 h; superoxide scavenger) inhibited norepinephrine (NE) (20 μM; 1 h)-induced vascular adventitial fibroblast (VAF) proliferation in WKY and SHR rats.

    Tempol purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Jul 9:S0891-5849(24)00552-5.  [Abstract]

    Comparison of the protective effect of MitoTEMPO (24 h; HY-112879) and Tempol (1.25-20 μM; 24 h) on erastin-induced ferroptotic cell death (MTT assay). The results showed that Tempol at a concentration of 20 μM exhibited protective effects on cells.

    Tempol purchased from MedChemExpress. Usage Cited in: Free Radic Biol Med. 2024 Jul 9:S0891-5849(24)00552-5.  [Abstract]

    Effect of MitoTEMPO and Tempol (5-40 μM) on cellular ROS and mitochondrial ROS levels in erastin-treated cells (fluorescence microscopy, scale bar = 100 μm). Cells were treated with 2.5 μM erastin with or without other drugs as indicated. The staining for cellular ROS, mitochondrial lipid-ROS, and mitochondrial ROS was carried out at 8, 10 and 12 h, respectively, following erastin exposure. The results showed that at a concentration of 40 μM, Tempol effectively reduced erastin-induced accumulation of intracellular ROS, mitochondrial ROS, and lipid ROS.

    Tempol purchased from MedChemExpress. Usage Cited in: Redox Biol. 2021 Oct:46:102115

    Tempol (100 μM; 24 h, ROS scavenger) only blocked the TMAO effects on cytoplasmic superoxide.

    Tempol purchased from MedChemExpress. Usage Cited in: J Cell Mol Med. 2020 Sep;24(17):9545-9559.  [Abstract]

    After 1-h pre-treatment with Tempol (3 mM), RNF4 expression is measured by Western blot after H2O2 or ATO treatment in NMCMs. RNF4 elevated initially and then attenuated after H2O2 or ATO treatment, while Tempol partially reversed the oxidative stimulus-induced elevation of RNF4 in vitro.

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    • 参考文献

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    製品説明

    Tempol is a general superoxide dismutase (SOD)-mimetic agent that efficiently neutralizes reactive oxygen species (ROS).

    IC50 & Target

    ROS[1]
    体外実験

    Tempol significantly attenuates H2O2-mediated decrease in mitochondrial respiration and increase in LDH release from rat PT cells, indicating a reduction in cell injury and death. The beneficial actions of Tempol are similar to those obtained using the Fe2+ chelator DEF. However, coadministration of DEF and Tempol does not produce any additional beneficial actions against renal ischemia/reperfusion injury or against oxidative stress-mediated PT cell injury/death[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Tempol 関連抗体
    体内実験

    SOD-mimetic Tempol is able to mimic resveratrol’s effects on heart function. Tempol is administered daily by gavage. Mice treated with Met or Tmp had decreased PR and QTc intervals and increased heart rates compared to peroral vehicle (VEH). These results are similar to that obtained by treatment with RSV. Pre- and post-treatment profiles of individual mice are illustrated[1]. Tempol, a membrane-permeable radical scavenger, reduces oxidant stress-mediated renal dysfunction and injury in the rat. Tempol significantly reduces the increase in urea, creatinine, γGT, AST, NAG, and FENa produced by renal ischemia/reperfusion, suggesting an improvement in both renal function and injury. Tempol also significantly reduces kidney MPO activity and MDA levels, indicating a reduction in PMN infiltration and lipid peroxidation, respectively. Tempol reduces the histologic evidence of renal damage associated with ischemia/reperfusion and caused a substantial reduction in the staining for nitrotyrosine and PARS, suggesting reduced nitrosative and oxidative stress[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    臨床実験
    分子量

    172.24

    分子式

    C9H18NO2
    CAS 番号
    Appearance

    Solid

    Color

    Orange to red

    SMILES

    [O]N1C(C)(C)CC(O)CC1(C)C
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件

    Store at room temperature 3 years

    *In solvent : -80°C, 6 months; -20°C, 1 month (Store at room temperature 3 years)
    溶剤 & 溶解度
    体外: 

    H2O : ≥ 100 mg/mL (580.59 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 5.8059 mL 29.0293 mL 58.0585 mL
    5 mM 1.1612 mL 5.8059 mL 11.6117 mL
    10 mM 0.5806 mL 2.9029 mL 5.8059 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

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    体積 （開始）

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    体内:

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 25 mg/mL (145.15 mM); Clear solution; Need ultrasonic

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    純度とドキュメンテーション

    純度: 99.78%

    COA (249 KB) MS (188 KB) GC (231 KB)

    取扱説明書 (2659 KB)
    参考文献
    細胞実験
    [2]

    To investigate the effect of Tempol, DEF, and DEF coadministered with Tempol on H2O2-mediated cell injury and death, confluent cultures of PT cells are preincubated (10 min at 37°C) with Tempol (0.03 to 10 mM), DEF (0.03 to 10 mM), or DEF (3 mM) in combination with Tempol (3 mM). The ranges of concentrations of Tempol and DEF are based on those previously shown in this laboratory to reduce on H2O2-mediated cell injury and death in (1) cultured rat cardiac myoblasts (Tempol) and (2) primary cultures of rat PT cells (DEF ). PT cell cultures are then incubated with H2O2 (1 mM) for four hours, after which cellular injury and death are assessed. Upon completion of incubations, cellular injury and death are assessed using the spectrophotometric assays described later in this article[2].

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    動物実験
    [1][2]

    Mice[1]
    Female or male BALB/c mice (5-7 weeks of age) are used. Mice are infected intraperitoneally (i.p.) with 102 blood trypomastigote forms of the type I Colombian strain of T. cruzi. Treatments are performed daily for 30 days from the establishment of CCC (60 dpi) by i.p. injection of 15 mg/kg trans-resveratrol (10% ethanol/PBS), vehicle (10% ethanol/PBS), 5 mg/Kg EX527 (0.1% DMSO), or peroral administration of 40 mg/Kg Resveratrol (10%ethanol-PBS), 500 mg/kg Metformin (dissolved in water), 100 mg/kg Tempol (dissolved in water), Benznidazole (25 mg/Kg, dissolved in water) and vehicle (water or 10%ethanol-PBS).
    Rats[2]
    83 male Wistar rats weighing 230 to 320 g are used.Upon completion of surgical procedures, the animals are randomly allocated to the eight groups. At one minute before commencement of reperfusion, animals received a bolus injection of either vehicle (saline, 4 mL/kg, IV), Tempol (30 mg/kg in saline, IV), DEF (40 mg/kg in saline, IV), or DEF (40 mg/kg in saline, IV) in combination with Tempol (30 mg/kg in saline, IV). The corresponding groups then received a continuous infusion of one of the following throughout the reperfusion period: vehicle (saline, 4 mL/kg/h, IV), Tempol (30 mg/kg/h in saline, IV), DEF (40 mg/kg/h in saline, IV), or Tempol and DEF in combination (30 and 40 mg/kg/h, respectively, in saline, IV). To elucidate the effects of Tempol or DEF on cardiovascular hemodynamics and organ function in sham-operated rats, respective groups of animals received the treatments described previously in this article and as outlined. The concentration of Tempol administered in vivo is based on those previously demonstrated by us to provide significant protection against myocardial ischemia/reperfusion injury in an in vivo rat model. Similarly, the concentration of DEF used is identical to that which we have previously used to provide significant protection against hepatic ischemia/reperfusion injury in in vivo rat and rabbit models.

    MedChemExpress (MCE) はこれらの方法の精度を確認していません。 こちらは参照専用です。
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 5.8059 mL 29.0293 mL 58.0585 mL 145.1463 mL
    5 mM 1.1612 mL 5.8059 mL 11.6117 mL 29.0293 mL
    10 mM 0.5806 mL 2.9029 mL 5.8059 mL 14.5146 mL
    15 mM 0.3871 mL 1.9353 mL 3.8706 mL 9.6764 mL
    20 mM 0.2903 mL 1.4515 mL 2.9029 mL 7.2573 mL
    25 mM 0.2322 mL 1.1612 mL 2.3223 mL 5.8059 mL
    30 mM 0.1935 mL 0.9676 mL 1.9353 mL 4.8382 mL
    40 mM 0.1451 mL 0.7257 mL 1.4515 mL 3.6287 mL
    50 mM 0.1161 mL 0.5806 mL 1.1612 mL 2.9029 mL
    60 mM 0.0968 mL 0.4838 mL 0.9676 mL 2.4191 mL
    80 mM 0.0726 mL 0.3629 mL 0.7257 mL 1.8143 mL
    100 mM 0.0581 mL 0.2903 mL 0.5806 mL 1.4515 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Tempol Related Classifications

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    一般には略語で表示されます：C1V1 = C2V2

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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Tempol2226-96-24-Hydroxy-TEMPOReactive Oxygen Species (ROS)AutophagyInhibitorinhibitorinhibit

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