Z-Gly-Gly-Arg-AMC acetate

Name: Z-Gly-Gly-Arg-AMC acetate
Brand: MedChemExpress
SKU: HY-P0019A
Rating: 5.0 (11 reviews)

Cat. No.: HY-P0019A Pureza: 99.78%: Ficha de datos
COA

SDS
Instrucciones de manejo
FAQs
Technical Support

Z-Gly-Gly-Arg-AMC acetate is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP) (Ex/Em = 390/480 nm).

Para uso exclusivo en investigación. No vendemos a pacientes.

Síntesis de péptidos personalizados

Z-Gly-Gly-Arg-AMC acetate Estructura química

No. CAS : 2070009-61-7

Tamaño	Stock	Cantidad
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

or Consulta para venta a granel

* Seleccione Cantidad antes de agregar artículos.

This product is a controlled substance and not for sale in your territory.

Revisión del cliente

Based on 11 publication(s) in Google Scholar

Other Forms of Z-Gly-Gly-Arg-AMC acetate:

Z-Gly-Gly-Arg-AMC In-stock
Z-Gly-Gly-Arg-AMC TFA Obtener un presupuesto

11 Publications Citing Use of MCE Z-Gly-Gly-Arg-AMC acetate

Recomendaciones destacadas

•Related Small Molecules:

•Related Proteins:

Actividad biológica
Pureza y Documentación
Referencias
Revisión del cliente

Descripciòn

Z-Gly-Gly-Arg-AMC acetate is a thrombin-specific fluorogenic substrate for testing of thrombin generation in PRP and platelet-poor plasma (PPP) (Ex/Em = 390/480 nm)^[1]^[2].

In Vitro

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation^[3]
1.1 Preparation of stock solution
Solvent: H2O
Concentration: 10 mM.
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 10 μM with PBS (or other buffer) (optimized according to the experiment).
Note: The working solution should be prepared and used immediately. Keep it away from light.

2. Thrombin inhibition screen
2.1 The samples and thrombin are transferred to a 384-well plate in sequence.
2.2 Add Z-Gly-Gly-Arg-AMC (50 μM) after incubation for 15 minutes.
2.3 Using a buffer containing 10 mM Tris-Cl (pH 7.4), 150 mM NaCl, 10 mM MgCl2, 1 mM CaCl2, 0.1% w/v BSA, 0.01% v/v Triton-X100, and 1% v/v DMSO and the fluorogenic substrate Z-Gly-Gly-Arg-AMC (50 μM).
2.4 The fluorescence intensity is measured with a fluorescence plate reader (Ex/Em = 360/465 nm) at 25°C for a period of 30 min with a read every 3 min.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

639.66

Fòrmula

C₃₀H₃₇N₇O₉

No. CAS

2070009-61-7

Appearance

Solid

Color

White to off-white

Emission (Em)

480

Excitation (Ex)

390

Sequence

Z-Gly-Gly-Arg-AMC

Sequence Shortening

ZGGR-AMC

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Sealed storage, away from moisture and light

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (156.33 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 20 mg/mL (31.27 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.5633 mL	7.8167 mL	15.6333 mL
5 mM	0.3127 mL	1.5633 mL	3.1267 mL
10 mM	0.1563 mL	0.7817 mL	1.5633 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Pureza y Documentación

Purity: 99.84%

Select Batch:

Ficha de datos (275 KB) SDS (252 KB)

COA (248 KB) HNMR (139 KB) LCMS (480 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Tarandovskiy ID, et al. Antiplatelet agents can promote two-peaked thrombin generation in platelet rich plasma: mechanism and possible applications. PLoS One. 2013;8(2):e55688. [Content Brief]

[2]. Machlus KR, et al. Effects of tissue factor, thrombomodulin and elevated clotting factor levels on thrombin generation in the calibrated automated thrombogram. Thromb Haemost. 2009 Nov;102(5):936-44. [Content Brief]

[3]. Mothukuri GK, et al. Macrocycle synthesis strategy based on step-wise "adding and reacting" three components enables screening of large combinatorial libraries. Chem Sci. 2020 Jun 26;11(30):7858-7863. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	1.5633 mL	7.8167 mL	15.6333 mL	39.0833 mL
	5 mM	0.3127 mL	1.5633 mL	3.1267 mL	7.8167 mL
	10 mM	0.1563 mL	0.7817 mL	1.5633 mL	3.9083 mL
	15 mM	0.1042 mL	0.5211 mL	1.0422 mL	2.6056 mL
	20 mM	0.0782 mL	0.3908 mL	0.7817 mL	1.9542 mL
	25 mM	0.0625 mL	0.3127 mL	0.6253 mL	1.5633 mL
	30 mM	0.0521 mL	0.2606 mL	0.5211 mL	1.3028 mL
DMSO	40 mM	0.0391 mL	0.1954 mL	0.3908 mL	0.9771 mL
	50 mM	0.0313 mL	0.1563 mL	0.3127 mL	0.7817 mL
	60 mM	0.0261 mL	0.1303 mL	0.2606 mL	0.6514 mL
	80 mM	0.0195 mL	0.0977 mL	0.1954 mL	0.4885 mL
	100 mM	0.0156 mL	0.0782 mL	0.1563 mL	0.3908 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.