Anetumab  (Synonyms: Anti-MSLN Antibody)

Anetumab (Anti-MSLN Antibody) is a fully humanized anti mesothelin (MSLN) IgG1 antibody. Anetumab (Anti-MSLN Antibody) can be used to synthesize the ADC molecule Anetumab ravtansine (HY-141606). Anetumab (Anti-MSLN Antibody) can be used for the research of cancer^[1].

Human IgG1 lambda

Human IgG1 lambda1, Isotype Control

Human

Mesothelin

Anetumab ravtansine (HY-141606) is composed of Anetumab and the microtubule-targeting toxin DM4 (HY-12454) linked by a reducible disulfide bond^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

10232  [NCBI]

Q13421

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

143.66 kDa

1954758-84-9

Liquid

Colorless to light yellow

[Anetumab]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Human IgG1 lambda

• 
  Immobilized Mesothelin Protein, Human (303a.a, HEK293, His, HY-P70148) can bind Anetumab. The EC₅₀ for this effect is 4.33 ng/mL.
• 
  Flow cytometric analysis of 1X10⁶ Hela cells with Anetumab (HY-P99352, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG4 (S228P) kappa Isotype Control (HY-P99003, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

• [1]. Golfier S, et al. Anetumab ravtansine: a novel mesothelin-targeting antibody-drug conjugate cures tumors with heterogeneous target expression favored by bystander effect. Mol Cancer Ther. 2014 Jun;13(6):1537-48.  [Content Brief]