DM4 (Synonyms: Ravtansine)

Name: DM4
Brand: MedChemExpress
SKU: HY-12454
Rating: 5.0 (5 reviews)

Cat. No.: HY-12454 Purity: 99.64%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

DM4 is is an antitubulin agent that inhibit cell division. DM4 can be used in the preparation of antibody agent conjugate.

For research use only. We do not sell to patients.

CAS No. : 796073-69-3

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of DM4:

DM4-d₆ In-stock
Maytansinoid DM4 Get quote

5 Publications Citing Use of MCE DM4

Cell Proliferation/Viability Assay

Flow Cytometry

In Vivo Efficacy Study

: DM4 purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Apr 11;23(1):431. [Abstract]; In vitro IC₅₀ curves were assessed through cytotoxicity assays by exposing NXS2 CTRL and hLGALS3BP cells to increasing doses of payload DM4 (0.00064 to 250 nM; 72 h).

: DM4 purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Apr 11;23(1):431. [Abstract]; Surface levels of PD-L1 evaluated through FACS analysis expressed as fold change of mean fluorescence intensity signals of treated samples over controls of NXS2 control and hLGALS3BP cells treated with DM4 (3 nM; 72 h) or with DMA. DM4 has the ability to induce a potential resistance mechanism.

: DM4 purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Apr 11;23(1):431. [Abstract]; 1959-sss/DM4 (ADC) (10 mg/kg; twice weekly; three total i.v.) treatment resulted in a significant shrinkage of hLGALS3BP-expressing tumors in syngeneic A/J mice.

: DM4 purchased from MedChemExpress. Usage Cited in: ACS Omega. 2025 Oct 15;10(42):50382-50393. [Abstract]; 1959-sss/DM4 (ADC) (10 mg/kg; twice weekly; three total i.v.) significantly reduced metastatic lesion area, especially in mice injected with LGALS3BP-expressing hNB cells.

: DM4 purchased from MedChemExpress. Usage Cited in: Curr Issues Mol Biol. 2023 Oct 5;45(10):8112-8125. [Abstract]; Cytotoxic activity of pegylated antibody fragment-drug conjugates in GD2-positive B78-D14 cell line. scFv-PEG4-DM4 (0-370 nM; 72 h) was more effective than scFv-PEG4-DM1 in inhibiting cell growth because the cytotoxicity was approximately twice as high for the FDC loaded with DM4 as opposed to that with DM1.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

DM4 is is an antitubulin agent that inhibit cell division. DM4 can be used in the preparation of antibody agent conjugate.

In Vitro

DM4, a structural analogue of maytansine, is a new thiol-containing and potent maytansinoid. DM4 is a cytotoxic maytansinoid drug. It is synthesized in order to link maytansinoids to antibodies via disulfide bonds. Maytansinoids inhibit tubulin polymerization and microtubule assembly and enhance microtubule destabilization, so there is potent suppression of microtubule dynamics resulting in a mitotic block and subsequent apoptotic cell death^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Clinical Trial

Molecular Weight

780.37

Formula

C₃₈H₅₄ClN₃O₁₀S

CAS No.

796073-69-3

Appearance

Solid

Color

White to light yellow

SMILES

C[C@]1([C@H](CC(N(C(C=C2C=C3OC)=C3Cl)C)=O)OC([C@H](C)N(C)C(CCC(C)(S)C)=O)=O)[C@H]([C@@H]([C@](OC4=O)([H])C[C@]([C@](/C=C/C=C(C)/C2)([H])OC)(N4)O)C)O1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (128.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.2814 mL	6.4072 mL	12.8144 mL
5 mM	0.2563 mL	1.2814 mL	2.5629 mL
10 mM	0.1281 mL	0.6407 mL	1.2814 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (3.20 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.64%

Select Batch:

Data Sheet (275 KB) SDS (643 KB)

COA (251 KB) HNMR (327 KB) RP-HPLC (250 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Tang R, et al. P-gp activity is a critical resistance factor against AVE9633 and DM4 cytotoxicity in leukaemia cell lines, but not a major mechanism of chemoresistance in cells from acute myeloid leukaemia patients. BMC Cancer. 2009 Jun 23;9:199. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.2814 mL	6.4072 mL	12.8144 mL	32.0361 mL
	5 mM	0.2563 mL	1.2814 mL	2.5629 mL	6.4072 mL
	10 mM	0.1281 mL	0.6407 mL	1.2814 mL	3.2036 mL
	15 mM	0.0854 mL	0.4271 mL	0.8543 mL	2.1357 mL
	20 mM	0.0641 mL	0.3204 mL	0.6407 mL	1.6018 mL
	25 mM	0.0513 mL	0.2563 mL	0.5126 mL	1.2814 mL
	30 mM	0.0427 mL	0.2136 mL	0.4271 mL	1.0679 mL
	40 mM	0.0320 mL	0.1602 mL	0.3204 mL	0.8009 mL
	50 mM	0.0256 mL	0.1281 mL	0.2563 mL	0.6407 mL
	60 mM	0.0214 mL	0.1068 mL	0.2136 mL	0.5339 mL
	80 mM	0.0160 mL	0.0801 mL	0.1602 mL	0.4005 mL
	100 mM	0.0128 mL	0.0641 mL	0.1281 mL	0.3204 mL

DM4 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DM4796073-69-3RavtansineDM 4DM-4Microtubule/TubulinADC PayloadADC CytotoxinInhibitorinhibitorinhibit

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DM4 (Synonyms: Ravtansine)

Customer Review

Other Forms of DM4:

DM4 Related Antibodies

5 Publications Citing Use of MCE DM4

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All ADC Payload Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

DM4 Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

DM4 Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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