2. Cell Cycle/DNA Damage Cytoskeleton Antibody-drug Conjugate/ADC Related
  3. Microtubule/Tubulin ADC Payload
  4. DM4

DM4 is is an antitubulin agent that inhibit cell division. DM4 can be used in the preparation of antibody agent conjugate.

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CAS 番号 : 796073-69-3

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 155 在庫あり
Solution
10 mM * 1 mL in DMSO USD 155 在庫あり
Solid
5 mg $90 在庫あり
10 mg $165 在庫あり
25 mg $340 在庫あり
50 mg $550 在庫あり
100 mg $880 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 5 publication(s) in Google Scholar

Other Forms of DM4:

DM4 関連抗体

Top Publications Citing Use of Products

    DM4 purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Apr 11;23(1):431.  [Abstract]

    In vitro IC50 curves were assessed through cytotoxicity assays by exposing NXS2 CTRL and hLGALS3BP cells to increasing doses of payload DM4 (0.00064 to 250 nM; 72 h).

    DM4 purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Apr 11;23(1):431.  [Abstract]

    Surface levels of PD-L1 evaluated through FACS analysis expressed as fold change of mean fluorescence intensity signals of treated samples over controls of NXS2 control and hLGALS3BP cells treated with DM4 (3 nM; 72 h) or with DMA. DM4 has the ability to induce a potential resistance mechanism.

    DM4 purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Apr 11;23(1):431.  [Abstract]

    1959-sss/DM4 (ADC) (10 mg/kg; twice weekly; three total i.v.) treatment resulted in a significant shrinkage of hLGALS3BP-expressing tumors in syngeneic A/J mice.

    DM4 purchased from MedChemExpress. Usage Cited in: ACS Omega. 2025 Oct 15;10(42):50382-50393.  [Abstract]

    1959-sss/DM4 (ADC) (10 mg/kg; twice weekly; three total i.v.) significantly reduced metastatic lesion area, especially in mice injected with LGALS3BP-expressing hNB cells.

    DM4 purchased from MedChemExpress. Usage Cited in: Curr Issues Mol Biol. 2023 Oct 5;45(10):8112-8125.  [Abstract]

    Cytotoxic activity of pegylated antibody fragment-drug conjugates in GD2-positive B78-D14 cell line. scFv-PEG4-DM4 (0-370 nM; 72 h) was more effective than scFv-PEG4-DM1 in inhibiting cell growth because the cytotoxicity was approximately twice as high for the FDC loaded with DM4 as opposed to that with DM1.

    ADC Payload アイソフォーム固有の製品をすべて表示:

    すべてのアイソフォームを表示
    Auristatin Calicheamicins Camptothecins Daunorubicins/Doxorubicins Duocarmycins Maytansinoids Pyrrolobenzodiazepines Traditional Cytotoxic Agents

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    DM4 is is an antitubulin agent that inhibit cell division. DM4 can be used in the preparation of antibody agent conjugate.

    体外実験

    DM4, a structural analogue of maytansine, is a new thiol-containing and potent maytansinoid. DM4 is a cytotoxic maytansinoid drug. It is synthesized in order to link maytansinoids to antibodies via disulfide bonds. Maytansinoids inhibit tubulin polymerization and microtubule assembly and enhance microtubule destabilization, so there is potent suppression of microtubule dynamics resulting in a mitotic block and subsequent apoptotic cell death[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    DM4 関連抗体
    臨床実験
    分子量

    780.37

    分子式

    C38H54ClN3O10S
    CAS 番号
    Appearance

    Solid

    Color

    White to light yellow

    SMILES

    C[C@]1([C@H](CC(N(C(C=C2C=C3OC)=C3Cl)C)=O)OC([C@H](C)N(C)C(CCC(C)(S)C)=O)=O)[C@H]([C@@H]([C@](OC4=O)([H])C[C@]([C@](/C=C/C=C(C)/C2)([H])OC)(N4)O)C)O1
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (128.14 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.2814 mL 6.4072 mL 12.8144 mL
    5 mM 0.2563 mL 1.2814 mL 2.5629 mL
    10 mM 0.1281 mL 0.6407 mL 1.2814 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.5 mg/mL (3.20 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (3.20 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.64%

    COA (251 KB) HNMR (327 KB) RP-HPLC (250 KB) MS (69 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.2814 mL 6.4072 mL 12.8144 mL 32.0361 mL
    5 mM 0.2563 mL 1.2814 mL 2.5629 mL 6.4072 mL
    10 mM 0.1281 mL 0.6407 mL 1.2814 mL 3.2036 mL
    15 mM 0.0854 mL 0.4271 mL 0.8543 mL 2.1357 mL
    20 mM 0.0641 mL 0.3204 mL 0.6407 mL 1.6018 mL
    25 mM 0.0513 mL 0.2563 mL 0.5126 mL 1.2814 mL
    30 mM 0.0427 mL 0.2136 mL 0.4271 mL 1.0679 mL
    40 mM 0.0320 mL 0.1602 mL 0.3204 mL 0.8009 mL
    50 mM 0.0256 mL 0.1281 mL 0.2563 mL 0.6407 mL
    60 mM 0.0214 mL 0.1068 mL 0.2136 mL 0.5339 mL
    80 mM 0.0160 mL 0.0801 mL 0.1602 mL 0.4005 mL
    100 mM 0.0128 mL 0.0641 mL 0.1281 mL 0.3204 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    DM4 Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    DM4796073-69-3RavtansineDM 4DM-4Microtubule/TubulinADC PayloadADC CytotoxinInhibitorinhibitorinhibit

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    Inquiry Information

    製品名:
    DM4
    製品番号:
    HY-12454
    数量:
    MCE 日本正規代理店:

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