Anti-DLL3 Antibody (anti-DLL3 arm derived from AMG-757) 

Anti-DLL3 Antibody (anti-DLL3 arm derived from AMG-757) is the anti-DLL3 arm of the bispecific T-cell engager (BiTE) antibody-Tarlatamab (AMG-757) (HY-P99575). Tarlatamab targets both DLL3 and CD3.

Human IgG1 kappa

Human IgG1 kappa, Isotype Control

Human

DLL3

10683  [NCBI]

Q9NYJ7

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

145.18 kDa

Liquid

Colorless to light yellow

[Anti-DLL3 Antibody (anti-DLL3 arm derived from AMG-757)]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Flow cytometric analysis of 1X10⁶ U118MG cells with Anti-DLL3 Antibody (HY-P990104, red). Cells were fixed with 4% paraformaldehyde. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. AF 488-conjugated AffiniPure Goat Anti-Human IgG H&L (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa (HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
• 
  Immobilized Delta-like protein 3/DLL3 Protein, Human (Biotinylated, HEK293, His, HY-P78115) can bind Anti-DLL3 Antibody (anti-DLL3 arm derived from AMG-757) . The EC₅₀ for this effect is 22.43 ng/mL.

• [1]. Giffin MJ, et al. AMG 757, a Half-Life Extended, DLL3-Targeted Bispecific T-Cell Engager, Shows High Potency and Sensitivity in Preclinical Models of Small-Cell Lung Cancer. Clin Cancer Res. 2021 Mar 1;27(5):1526-1537.  [Content Brief]