Syndecan 1 Antibody (YA1490)

製品番号: HY-P81745: データシート SDS COA User Guide for Antibodies
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Syndecan 1 Antibody (YA1490) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Syndecan 1.

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容量	価格（税別）	在庫状況	数量
10 μL	$90	在庫あり	Estimated Time of Arrival: December 31
50 μL	$240	在庫あり	Estimated Time of Arrival: December 31
100 μL	$390	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

or バルクお問い合わせ

* アイテムを追加する前、数量をご選択ください

顧客検証

•Probiotics Antimicrob Proteins. 2024 Aug 22. [Abstract]

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明

製品説明

Syndecan 1 Antibody (YA1490) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Syndecan 1.

Host

Rabbit

Clonality

Monoclonal,Recombinant

分子量

Predicted band size: 33 kDa;

Observed band size: 37/100 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P18827

Gene ID

6382 [NCBI]

Immunogen

Recombinant protein within Human Syndecan 1 aa 13-274 / 310.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:2000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:200
FC FC: Flow Cytometry	1:1000

Sensitivity	Endogenous	純度	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

1.Supplied in PBS with 0.05% sodium azide.
2.Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Please refer to the lot-specific COA for specific buffer information.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

ALL FC ICC

Flow cytometric analysis of 1X10^6 Hela cells labeling Syndecan 1 Antibody (HY-P81745, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).
Immunocytochemistry analysis of A431 cells labeling Syndecan 1 with Syndecan 1 Antibody (HY-P81745) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Syndecan 1 Antibody (HY-P81745) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of A431 cells labeling Syndecan 1 with Syndecan 1 Antibody (HY-P81745) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Syndecan 1 Antibody (HY-P81745) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Cell surface proteoglycan that contains both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix (By similarity). Regulates exosome biogenesis in concert with SDCBP and PDCD6IP (PubMed:22660413). Able to induce its own expression in dental mesenchymal cells and also in the neighboring dental epithelial cells via an MSX1-mediated pathway (By similarity)

Subcellular Localization：Membrane; Single-pass type I membrane protein; Secreted; Secreted, extracellular exosome

Expression：
Tissue_specificity:Protein levels were detected in the placenta (PubMed: 32337544) . Protein levels were detected in fibroblasts (PubMed: 36213313) .
Positive sample: HeLa cell lysate, A431 cell lysate, Daudi cell lysate, Raji cell lysate, Ramos cell lysate, A431, Hela, HepG2, human tonsil tissue, human kidney tissue,

Subunit：Interacts with CDCP1. Interacts (via C-terminus) with TIAM1 (via PDZ domain). Interacts with MDK (By similarity)

RRID

AB_3103851

Database

Entrez Gene: 6382 Human ; 20969 Mouse ; 25216 Rat

SwissProt: P18827 Human ; P18828 Mouse ; P26260 Rat

OMIM: 186355 Human

Research Field

Cell Biology

Synonyms

SDC1; SDC; Syndecan-1; SYND1; CD138

ドキュメンテーション

Select Batch:

データシート (234 KB) SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)