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  5. Syndecan 1 Antibody (YA1490)

Syndecan 1 Antibody (YA1490)

Cat. No.: HY-P81745
COA
SDS
User Guide for Antibodies Technical Support

Syndecan 1 Antibody (YA1490) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Syndecan 1.

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Top Publications Citing Use of Products

1 Publications Citing Use of MCE Syndecan 1 Antibody (YA1490)

  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

  • Verification Image

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  • Description

Description

Syndecan 1 Antibody (YA1490) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Syndecan 1.
Host

Rabbit
Clonality

Monoclonal,Recombinant
Masse moléculaire
Predicted band size: 33 kDa;
Observed band size: 37/100 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human, Mouse, Rat
SwissProt ID
Gene ID
Immunogen

Recombinant protein within Human Syndecan 1 aa 13-274 / 310.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:2000
ICC/IF
ICC/IF: Immunocytochemistry/Immunofluorescence
1:100
IF-Tissue
IF-Tissue: Immunofluorescence-Tissue
1:50
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:50-1:200
FC
FC: Flow Cytometry
1:1000
Sensitivity Endogenous Pureté affinity purified
Conjugation Non-conjugated Modification Unmodified
Isotype IgG  
Appearance

Liquid
Formulation

1.Supplied in PBS with 0.05% sodium azide.
2.Supplied in TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Please refer to the lot-specific COA for specific buffer information.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Livraison

Shipping with blue ice.
Verification Image
ALL FC ICC

  • Flow cytometric analysis of 1X10^6 Hela cells labeling Syndecan 1 Antibody (HY-P81745, red). Cells were stained with the primary antibody at 1/100 dilution for an hour at 4℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L (HY-P8002) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Rabbit IgG Isotype Control (HY-P80879, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

  • Immunocytochemistry analysis of A431 cells labeling Syndecan 1 with Syndecan 1 Antibody (HY-P81745) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Syndecan 1 Antibody (HY-P81745) at 1/50 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

  • Immunocytochemistry analysis of A431 cells labeling Syndecan 1 with Syndecan 1 Antibody (HY-P81745) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with BSA for Immunol Staining for 10 min at room temperature. Cells were then incubated with Syndecan 1 Antibody (HY-P81745) at 1/100 dilution in BSA for Immunol Staining at 4 ℃ overnight. AF488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background
Function：Cell surface proteoglycan that contains both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix (By similarity). Regulates exosome biogenesis in concert with SDCBP and PDCD6IP (PubMed:22660413). Able to induce its own expression in dental mesenchymal cells and also in the neighboring dental epithelial cells via an MSX1-mediated pathway (By similarity)
Subcellular Localization：Membrane; Single-pass type I membrane protein; Secreted; Secreted, extracellular exosome
Expression：
Tissue_specificity:Protein levels were detected in the placenta (PubMed: 32337544) . Protein levels were detected in fibroblasts (PubMed: 36213313) .
Positive sample: HeLa cell lysate, A431 cell lysate, Daudi cell lysate, Raji cell lysate, Ramos cell lysate, A431, Hela, HepG2, human tonsil tissue, human kidney tissue,
Subunit：Interacts with CDCP1. Interacts (via C-terminus) with TIAM1 (via PDZ domain). Interacts with MDK (By similarity)
RRID
Database

Entrez Gene: 6382 Human ; 20969 Mouse ; 25216 Rat

SwissProt: P18827 Human ; P18828 Mouse ; P26260 Rat

OMIM: 186355 Human

Research Field

Cell Biology
Synonyms
SDC1; SDC; Syndecan-1; SYND1; CD138
Documentation
SDS (251 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)

Syndecan 1 Antibody (YA1490) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Syndecan 1 Antibody (YA1490)
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HY-P81745
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