delta Tubulin Antibody

Cat. No.: HY-P83669: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

delta Tubulin Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to delta Tubulin.

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사이즈	재고	수량
10 μL	해외재고보유	Estimated Time of Arrival: December 31
50 μL	해외재고보유	Estimated Time of Arrival: December 31
100 μL	해외재고보유	Estimated Time of Arrival: December 31
250 μL	견적 받기

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delta Tubulin Antibody Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
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delta Tubulin Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to delta Tubulin.

Host

Rabbit

Clonality

Polyclonal

분자량

Predicted band size: 51 kDa;

Observed band size: 51 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse

SwissProt ID

Q9UJT1

Gene ID

51174 [NCBI]

Immunogen

Fusion protein of human TUBD1.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100

Sensitivity	Endogenous	Purity	Protein A affinity purified.
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in pH 7.4 PBS, 0.05% NaN3, 40% Glycerol.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

선적

Shipping with blue ice.

Verification Image

ALL WB IHC-P

Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using delta Tubulin Antibody(HY-P83669) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using delta Tubulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83669, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using delta Tubulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83669, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Background

Function：Acts as a positive regulator of hedgehog signaling and regulates ciliary function

Subcellular Localization：Nucleus; Cytoplasm; Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole; Cell projection, cilium

Isoforms & Post-Translational Modification：Q9UJT1 has 6 isomers: Q9UJT1-1: 51034 Da (predicted); Q9UJT1-2: 44658 Da (predicted); Q9UJT1-3: 22381 Da (predicted); Q9UJT1-4: 27074 Da (predicted); Q9UJT1-5: 44555 Da (predicted); Q9UJT1-6: 39525 Da (predicted).

Subunit：Found in a complex with TEDC1, TEDC2, TUBE1 and TUBD1

RRID

AB_3676577

Database

Entrez Gene: 51174 Human ; 56427 Mouse ;

SwissProt: Q9UJT1 Human ; Q9R1K7 Mouse ;

OMIM: 607344 Human

Research Field

Signal Transduction

Synonyms

TUBD1,TUBD,FLJ12709,TBD_HUMAN antibody,Tubulin delta chain antibod

각종 서류

Select Batch:

Data Sheet (231 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

delta Tubulin Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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상품명:: delta Tubulin Antibody
Cat. No.:: HY-P83669
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1/16

MedchemExpress Validation
Western blot analysis of extracts from Jurkat(lane 2(20ug) and Jurkat(lane 3(40ug) using delta Tubulin Antibody(HY-P83669) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
1/16

MedchemExpress Validation
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using delta Tubulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83669, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
1/16

MedchemExpress Validation
Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using delta Tubulin Antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HY-P83669, 1/100) in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
1/16

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delta Tubulin Antibody

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delta Tubulin Antibody Related Classifications

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