GADD45A Antibody (YA1076)

製品番号: HY-P81331: データシート SDS COA User Guide for Antibodies
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GADD45A Antibody (YA1076) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GADD45A.

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容量	価格（税別）	在庫状況	数量
10 μL	$90	在庫あり	Estimated Time of Arrival: December 31
50 μL	$228	在庫あり	Estimated Time of Arrival: December 31
100 μL	$372	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明
参考文献

製品説明

GADD45A Antibody (YA1076) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to GADD45A.

Host

Mouse

Clonality

Monoclonal

分子量

Predicted band size: 18 kDa;

Observed band size: 22 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P24522

Gene ID

1647 [NCBI]

Immunogen

Purified recombinant human GADD45 α protein expressed in E.coli

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Transfected	純度	Affinity Purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Jurkat(lane 2(20ug) , NIH/3T3(lane 3(20ug) and HEK293(lane 4(20ug) using GADD45A Antibody (HY-P81331) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity (By similarity). Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase

Subcellular Localization：Nucleus

Expression：
Induction:By UV irradiation, X-rays, growth arrest and alkylating agents. The induction is mediated by some kinase (s) other than PKC

Isoforms & Post-Translational Modification：P24522 has 2 isomers: P24522-1: 18336 Da (predicted); P24522-2: 14622 Da (predicted).

Subunit：Interacts with MAPK14 (By similarity). Predominantly monomeric but also forms dimers and other oligomers as concentration increases. Interacts with GADD45GIP1. Interacts weakly with PCNA. Interacts with AURKA, likely to compete with dimerization

RRID

AB_3103126

Database

Entrez Gene: 1647 Human ; 13197 Mouse ;

SwissProt: P24522 Human ; P48316 Mouse ;

OMIM: 126335 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

DDIT1; GADD45

ドキュメンテーション

Select Batch:

データシート (232 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/rpmi-2650-cell.html