KDM1/LSD1 Antibody (YA718)

製品番号: HY-P80731: データシート SDS COA User Guide for Antibodies
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KDM1/LSD1 Antibody (YA718) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to KDM1/LSD1.

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容量	価格（税別）	在庫状況	数量
無料サンプル		今すぐ申し込む
10 μL	$84	在庫あり	Estimated Time of Arrival: December 31
50 μL	$222	在庫あり	Estimated Time of Arrival: December 31
100 μL	$360	在庫あり	Estimated Time of Arrival: December 31
250 μL		お問い合わせ

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* アイテムを追加する前、数量をご選択ください

おすすめ:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Verification Image
Background
説明
参考文献

製品説明

KDM1/LSD1 Antibody (YA718) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to KDM1/LSD1.

Host

Mouse

Clonality

Monoclonal

分子量

Predicted band size: 93 kDa;

Observed band size: 95-110 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Monkey

SwissProt ID

O60341

Gene ID

23028 [NCBI]

Immunogen

Synthetic peptide corresponding to Human LSD1.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	純度	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

輸送条件

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Jurkat(lane 2(40μg), HCT116(lane 3(40μg)) and NIH3T3(lane 4(40μg)), using KDM1A (HY-P80731) Mouse mAb. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in TBST for 2 hour at room temperature. The primary antibody (HY-P80731, 1/1000) and Loading control antibody (GAPDH, HY-P80954, 1/3000) was used in 5% BSA in TBST at 4°C overnight. Goat Anti-Mouse IgG-HRP Secondary Antibody (HY-P8001/HY-P8004, 1/10,000) was used for 1 hour at room temperature.

Background

Function：Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context (PubMed:15620353, PubMed:15811342, PubMed:16079794, PubMed:16079795, PubMed:16140033, PubMed:16223729, PubMed:27292636). Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed (PubMed:15620353, PubMed:15811342, PubMed:16079794, PubMed:21300290). Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me (PubMed:15620353, PubMed:20389281, PubMed:21300290, PubMed:23721412). May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity (PubMed:16079794, PubMed:16140033, PubMed:16885027, PubMed:21300290, PubMed:23721412). Also acts as a coactivator of androgen receptor (AR)-dependent transcription, by being recruited to AR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in AR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A (PubMed:16079795). Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1 (PubMed:29691401). Demethylates methylated 'Lys-42' and methylated 'Lys-117' of SOX2 (PubMed:29358331). Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development (PubMed:16079794, PubMed:16140033). Facilitates epithelial-to-mesenchymal transition by acting as an effector of SNAI1-mediated transcription repression of epithelial markers E-cadherin/CDH1, CDN7 and KRT8 (PubMed:20562920, PubMed:27292636). Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7 (PubMed:20389281). Required for the repression of GIPR expression (PubMed:34655521, PubMed:34906447)

Subcellular Localization：Nucleus; Chromosome

Expression：
Tissue_specificity:general expression

Induction:Down-regulated during neural differentiation in neuroblastoma cancer

Isoforms & Post-Translational Modification：O60341 has 2 isomers: O60341-1: 92903 Da (predicted); O60341-2: 95155 Da (predicted).
Acetylated by KAT8 in epithelial but not in mesenchymal cells, thereby regulating the epithelial-to-mesenchymal transition (PubMed:27292636). Acetylation by KAT8 reduces KDM1A association with nucleosomes, thereby decreasing histone H3 demethylation, leading to transcription activatio of target genes (PubMed:27292636);Polyubiquitinated by JADE2; which leads to its proteasomal degradation (PubMed:25018020). Deubiquitinated by USP38; preventing it from degradation by the 26S proteasome (PubMed:30497519)

Subunit：Component of a histone demethylase complex with RCOR1 (PubMed:16885027, PubMed:20389281, PubMed:21300290, PubMed:23721412). Component of a RCOR/GFI/KDM1A/HDAC complex (PubMed:11102443, PubMed:12032298).

RRID

AB_3102178

Database

Entrez Gene: 23028 Human ; 99982 Mouse ; 500569 Rat

SwissProt: O60341 Human ; Q6ZQ88 Mouse ;

OMIM: 616728 Human

Research Field

Epigenetics and Nuclear Signaling

Synonyms

KDM1A; AOF2; KDM1; KIAA0601; LSD1; Lysine-specific histone demethylase 1A; BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2

ドキュメンテーション

Select Batch:

データシート (232 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

参考文献

[1]. /biology-dictionary/a549-cell.html [Content Brief]