Lactate Dehydrogenase A Antibody (YA317)

Cat. No.: HY-P80738: Ficha de datos COA
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Lactate Dehydrogenase A Antibody (YA317) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Lactate Dehydrogenase A.

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Tamaño	Stock	Cantidad
20 μL	En stock	Estimated Time of Arrival: December 31
50 μL	En stock	Estimated Time of Arrival: December 31
100 μL	En stock	Estimated Time of Arrival: December 31
250 μL	Obtener un presupuesto

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Lactate Dehydrogenase A Antibody (YA317) Recomendaciones destacadas:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Descripciòn

Descripciòn

Lactate Dehydrogenase A Antibody (YA317) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to Lactate Dehydrogenase A.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Peso molecular

Predicted band size: 37 kDa;

Observed band size: 37 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Rat

SwissProt ID

P00338

Gene ID

3939 [NCBI]

Immunogen

Synthetic peptide corresponding to Human Lactate Dehydrogenase.The exact sequence is proprietary to MCE.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:50-1:100
IHC-F IHC-F: Immunohistochemistry-Frozen	1:50-1:100
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-1:200
IP IP: Immunoprecipitation	1:20

Sensitivity	Endogenous	Pureza	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol and 0.05% BSA. Preservative: 0.01% Sodium azide

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Envío

Shipping with blue ice.

Verification Image

ALL WB IHC-P ICC

Western blot analysis of extracts from RAW264.7(lane 2(20ug)，C6(lane 3(20ug) and NIH/3T3(lane 4(20ug) using Lactate Dehydrogenase A Antibody (HY-P80738) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Western blot analysis of extracts from Jurkat(lane 2(20ug) , C6(lane 3(20ug) and Hela(lane 4(20ug) using Lactate Dehydrogenase A Antibody (HY-P80738) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P83730, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.
Immunohistochemical analysis of paraffin-embedded rat liver tissue using Lactate Dehydrogenase A Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunohistochemical analysis of paraffin-embedded rat liver tissue using Lactate Dehydrogenase A Antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 8 minutes. The tissues were blocked in QuickBlock for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution in 4℃ overnight. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Immunocytochemistry analysis of Hela cells labeling Lactate Dehydrogenase A with Lactate Dehydrogenase A Antibody (HY-P80738)at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lactate Dehydrogenase A Antibody (HY-P80738) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).
Immunocytochemistry analysis of NIH/3T3 cells labeling Lactate Dehydrogenase A with Lactate Dehydrogenase A Antibody (HY-P80738) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 for 10 minutes at room temperature, then blocked with QuickBlock™ Blocking Buffer for Immunol Staining for 10 min at room temperature. Cells were then incubated with Lactate Dehydrogenase A Antibody (HY-P80738) at 1/50 dilution in QuickBlock™ Blocking Buffer for Immunol Staining at 4 ℃. Alexa Fluor® 488-conjugated AffiniPure Goat Anti-Rabbit IgG H&L（HY-P8002, Green) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. The Nuclear counterstain was DAPI (Blue).

Background

Function：Interconverts simultaneously and stereospecifically pyruvate and lactate with concomitant interconversion of NADH and NAD(+)

Subcellular Localization：Cytoplasm

Expression：
Tissue_specificity:It is mainly expressed in anaerobic tissues such as skeletal muscle and liver.

Isoforms & Post-Translational Modification：P00338 has 5 isomers: P00338-1: 36689 Da (predicted); P00338-2: 30057 Da (predicted); P00338-3: 39837 Da (predicted); P00338-4: 30205 Da (predicted); P00338-5: 26712 Da (predicted).
ISGylated

Subunit：Homotetramer (PubMed:11276087). Interacts with PTEN upstream reading frame protein MP31 (PubMed:33406399). Interacts with folliculin FLCN; the interaction is direct and inhibits enzymatic activity (PubMed:34381247)

RRID

AB_3102686

Database

Entrez Gene: 3939 Human ; 3945 Human ; 16828 Mouse ; 16832 Mouse ; 24533 Rat 24534

SwissProt: P00338 Human ; P07195 Human ; P07864 Human ; P06151 Mouse ; P16125 Mouse ; P04642 Rat P42123

OMIM: 612933 Human

Research Field

Signal Transduction

Synonyms

LDHA; LDH-M; LDH1; PIG19; GSD11; TRG5; Proliferation inducing gene 19

Documentación

Select Batch:

Ficha de datos (234 KB) SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Lactate Dehydrogenase A Antibody (YA317) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Lactate Dehydrogenase A Antibody (YA317)

Lactate Dehydrogenase A Antibody (YA317) Recomendaciones destacadas:

Product Detail

Verification Image

Background

Descripciòn

Lactate Dehydrogenase A Antibody (YA317) Related Classifications

Productos vistos recientemente:

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