LC3A/B Antibody

Cat. No.: HY-P80741: Data Sheet COA
SDS
User Guide for Antibodies
FAQs
Technical Support

LC3A/B Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to LC3A/B.

For research use only. We do not sell to patients.

Size	Stock	Quantity
20 μL	In-stock	Estimated Time of Arrival: December 31
50 μL	In-stock	Estimated Time of Arrival: December 31
100 μL	In-stock	Estimated Time of Arrival: December 31
250 μL	Get quote

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LC3A/B Antibody Featured Recommendations:

WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
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Background
Documentation

Description

LC3A/B Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to LC3A/B.

Host

Rabbit

Clonality

Polyclonal

Molecular Weight

Predicted band size: 14 kDa;

Observed band size: 14/16 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

Q9H492 / Q9GZQ8

Gene ID

81631 [NCBI]

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:500-1:1000

Sensitivity	Endogenous	Purity	affinity purified
Conjugation	Non-conjugated	Modification	Unmodified
Isotype	IgG

Appearance

Liquid

Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Verification Image

Western blot analysis of extracts from Hela (lane 2(20μg), HEK293T (lane 3(20μg) and C2C12(lane 4(20μg) using LC3A/B (HY-P80741) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background

Function：Q9H492: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20713600, PubMed:24290141). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20713600). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed:31006537, PubMed:31006538)
Q9GZQ8: Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed:20418806, PubMed:23209295, PubMed:28017329). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed:23209295, PubMed:28017329). In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria (PubMed:22922758). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed:20418806, PubMed:23209295, PubMed:28017329). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (PubMed:24089205).

Subcellular Localization：Q9H492: Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor; Endomembrane system; Lipid-anchor; Cytoplasm, cytoskeleton
Q9GZQ8: Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor; Endomembrane system; Lipid-anchor; Mitochondrion membrane; Lipid-anchor; Cytoplasm, cytoskeleton; Cytoplasmic vesicle

Expression：
Tissue_specificity: Q9H492: Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes
Q9GZQ8: Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver

Isoforms & Post-Translational Modification：Q9H492 has 2 isomers: Q9H492-1: 14272 Da (predicted); Q9H492-2: 14493 Da (predicted).
The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, LC3-I (PubMed:15187094, PubMed:30661429). The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, LC3-II (PubMed:15187094). During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid (PubMed:33909989). ATG4 proteins also mediate the delipidation of PE-conjugated forms (PubMed:32686895, PubMed:33909989). In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy (PubMed:33909989). ATG4B constitutes the major protein for proteolytic activation (PubMed:30661429, PubMed:33909989). ATG4D is the main enzyme for delipidation activity (By similarity);(Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine (PubMed:23112293, PubMed:31722778, PubMed:32686895). RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS) (PubMed:33909989);Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE)

Subunit：Q9H492: Interacts with TP53INP1 and TP53INP2 (PubMed:19056683, PubMed:22470510). Directly interacts with SQSTM1; this interaction leads to MAP1LC3A recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy (PubMed:17580304).
Q9GZQ8: 3 different light chains, LC1 (a cleavage product of MAP1B) , LC2 (a cleavage product of MAP1A) and LC3 (produced by one of the MAP1LC3 genes) , can associate with the MAP1A or MAP1B heavy chains (By similarity).

RRID

AB_3102687

Database

Entrez Gene: 81631 Human ; 84557 Human ; 66734 Mouse ; 67443 Mouse ; 362245 Rat ; 64862 Rat

SwissProt: Q9GZQ8 Human ; Q9H492 Human ; Q91VR7 Mouse ; Q9CQV6 Mouse ; Q62625 Rat Q6XVN8

OMIM: 609604 Human

Research Field

Signal Transduction

Synonyms

LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3; MAP1LC3A; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3; MAP1LC3B

Documentation

Select Batch:

Data Sheet (235 KB) SDS (252 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

LC3A/B Antibody Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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LC3A/B Antibody

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LC3A/B Antibody Related Classifications

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