2. Antibodies
  3. Primary Antibodies
  4. Monoclonal Antibodies
  5. CDKN2A/p16INK4a Antibody (YA3494)

CDKN2A/p16INK4a Antibody (YA3494)

Art. -Nr.: HY-P80992
COA
SDS
User Guide for Antibodies Technical Support

CDKN2A/p16INK4a Antibody (YA3494) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDKN2A/p16INK4a.

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • Beschreibung

  • Verweise

Beschreibung

CDKN2A/p16INK4a Antibody (YA3494) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to CDKN2A/p16INK4a.
Host

Rabbit
Clonality

Monoclonal, Recombinant
Molekulargewicht
Predicted band size: 17 kDa;
Observed band size: 17 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Synthetic peptide.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:1000
IHC-P
IHC-P: Immunohistochemistry-Paraffin
1:1000
Sensitivity Endogenous Conjugation Non-conjugated
Modification Unmodified Isotype IgG
Appearance

Liquid
Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Versand

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from Hela(lane 2(20μg), HEK293 (lane 3(20μg) and HepG2 (lane 4(20μg) using CDKN2A/p16INK4a (HY-P80992) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein
Subcellular Localization：Cytoplasm; Nucleus
Expression：
Tissue_specificity:It is widely expressed, but not detected in the brain or skeletal muscle. Isomer 3 is pancreas-specific.
Positive sample: HeLa cell lysate, HepG2 cell lysate, HEK-293 cell lysate, NIH:OVCAR-3 cell lysate, human ovary carcinoma tissue.
Isoforms & Post-Translational Modification：P42771 has 6 isomers: P42771-1: 16533 Da (predicted); P42771-2: 11215 Da (predicted); P42771-3: 12083 Da (predicted); Q8N726-1: 13903 Da (predicted); P42771-4: 17883 Da (predicted); Q8N726-2: 8731 Da (predicted).
Phosphorylation seems to increase interaction with CDK4
Subunit：Heterodimer with CDK4 or CDK6. Predominant p16 complexes contained CDK6. Interacts with CDK4 (both 'T-172'-phosphorylated and non-phosphorylated forms); the interaction inhibits cyclin D-CDK4 kinase activity. Interacts with ISCO2
RRID
Database

Entrez Gene: 1029 Human

SwissProt: P42771 Human

OMIM: 155601 Human

Synonyms
ARF antibody; CDK4 inhibitor p16 INK4 antibody; CDK4I antibody; CDKN2 antibody; CDKN2A antibody; Cell cycle negative regulator beta antibody; CMM2 antibody; INK4 antibody; INK4a antibody; Melanoma p16 inhibits CDK4 antibody; MLM antibody; MTS1 antibody; Multiple tumor suppressor 1 antibody; p16 antibody; p16 INK4a antibody; p16INK4a antibody; p19 antibody; P19ARF antibody; TP16 antibody;
Dokumentation
SDS (254 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)
Verweise

CDKN2A/p16INK4a Antibody (YA3494) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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CDKN2A/p16INK4a Antibody (YA3494)
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