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  4. Polyclonal Antibodies Modification Specific Antibodies
  5. Phospho-Cyclin E1 (Thr395) Antibody

Phospho-Cyclin E1 (Thr395) Antibody

Art. -Nr.: HY-P80807
COA
SDS
User Guide for Antibodies Technical Support

Phospho-Cyclin E1 (Thr395) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-Cyclin E1 (Thr395).

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Top Publications Citing Use of Products
  • WB: Western Blot;
  • IHC-P: Immunohistochemistry-Paraffin;
  • IHC-F: Immunohistochemistry-Frozen;
  • ICC/IF: Immunocytochemistry/Immunofluorescence;
  • IF-Tissue: Immunofluorescence-Tissue;
  • mIHC: Multiplex Immunohistochemical;
  • IP: Immunoprecipitation;
  • ChIP: Chromatin Immunoprecipitation;
  • FC: Flow Cytometry;
  • ELISA: Enzyme Linked Immunosorbent Assay

  • Product Detail

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  • Beschreibung

Beschreibung

Phospho-Cyclin E1 (Thr395) Antibody is a Rabbit-derived and non-conjugated IgG polyclonal antibody, targeting to Phospho-Cyclin E1 (Thr395).
Host

Rabbit
Clonality

Polyclonal
Molekulargewicht
Predicted band size: 47 kDa;
Observed band size: 47 kDa
Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
Species Reactivity
Human
SwissProt ID
Gene ID
Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr395 of Human Cyclin E1.The exact sequence is proprietary to MCE.
Application &
Dilution Ratio
Application Dilution Ratio
WB
WB: Western Blot
1:500-1:1000
Sensitivity Endogenous Reinheit affinity purified
Conjugation Non-conjugated Modification Phosphorylated
Isotype IgG  
Appearance

Liquid
Formulation

Supplied in 1*PBS (pH 7.3), 50% glycerol and 0.5% BSA. Preservative: 0.02% sodium azide.
Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.
Versand

Shipping with blue ice.
Verification Image
WB

  • Western blot analysis of extracts from Hela(lane 2(20ug) and Hela(lane 3(40ug) using Phospho-Cyclin E1 Antibody (HY-P80807) Rabbit mAb. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 2 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (Beta Actin, HY-P80438, 1/10000) was used in 5% non-fat milk in TBST at 4°C overnight. Goat Anti-Mouse/Rabbit IgG-HRP Secondary Antibody (1/10000) was used for 1 hour at room temperature.

Background
Function：Essential for the control of the cell cycle at the G1/S (start) transition
Subcellular Localization：Nucleus
Expression：
Tissue_specificity:It is highly expressed in the testes and placenta. Its expression level is lower in bronchial epithelial cells.
Isoforms & Post-Translational Modification：P24864 has 3 isomers: P24864-1: 47077 Da (predicted); P24864-2: 41982 Da (predicted); P24864-3: 45150 Da (predicted).
Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7;Ubiquitinated by UHRF2; appears to occur independently of phosphorylation
Subunit：Interacts with CDK2 protein kinase to form a serine/threonine kinase holoenzyme complex. The cyclin subunit imparts substrate specificity to the complex (PubMed:15660127). Found in a complex with CDK2, CABLES1 and CCNA1 (By similarity). Part of a complex consisting of UHRF2, CDK2 and CCNE1 (PubMed:15178429). Interacts directly with UHRF2; the interaction ubiquitinates CCNE1 and appears to occur independently of CCNE1 phosphorylation (PubMed:21952639). Interacts with INCA1 (PubMed:21540187)
RRID
Database

Entrez Gene: 898 Human

SwissProt: P24864 Human

OMIM: 123837 Human

Research Field

Cell Biology
Synonyms
CCNE1; CCNE; G1/S-specific cyclin-E1
Dokumentation
SDS (251 KB)

COA (205 KB)

User Guide for Antibodies (1077 KB)

Phospho-Cyclin E1 (Thr395) Antibody Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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