2. Autophagy
  3. Atg8/LC3 Atg7 Autophagy
  4. ATG7-IN-3

ATG7-IN-3 (Compound 18) is a potent ATG7 inhibitor with an IC50 of 0.048 μM. ATG7-IN-3 inhibits Autophagy. ATG7-IN-3 inhibits the formation of LC3B puncta. ATG7-IN-3 can be used in the researches of glioma and colon cancer.

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ATG7-IN-3

ATG7-IN-3 構造式

CAS 番号 : 2226229-76-9

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 1595 在庫あり
Solution
10 mM * 1 mL in DMSO USD 1595 在庫あり
Solid
1 mg $580 在庫あり
5 mg $1450 在庫あり
10 mg $2350 在庫あり
50 mg   お問い合わせ  
100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 0 publication(s) in Google Scholar

ATG7-IN-3 関連抗体

Top Publications Citing Use of Products

  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

  • カスタマーレビュー

製品説明

ATG7-IN-3 (Compound 18) is a potent ATG7 inhibitor with an IC50 of 0.048 μM. ATG7-IN-3 inhibits Autophagy. ATG7-IN-3 inhibits the formation of LC3B puncta. ATG7-IN-3 can be used in the researches of glioma and colon cancer[1].

IC50 & Target

IC50: 0.048 ± 0.022 μM (ATG7), 0.280 ± 0.051 μM (Thioester), 0.938 ± 0.231 μM (LC3B)[1]
Cellular Effect
Cell Line Type Value Description References
NCI-H1650 EC50
10.4 3
Compound: 18
Antiproliferative activity against human H1650 cells assessed as reduction in cell viability after 72 hrs by cell titer glow assay
Antiproliferative activity against human H1650 cells assessed as reduction in cell viability after 72 hrs by cell titer glow assay
[PMID: 32912429]
NCI-H1650 EC50
10.4 3
Compound: 18
Antiproliferative activity against human H1650 cells assessed as reduction in cell viability after 72 hrs by cell titer glow assay
Antiproliferative activity against human H1650 cells assessed as reduction in cell viability after 72 hrs by cell titer glow assay
[PMID: 32912429]
NCI-H1650 EC50
10.4 3
Compound: 18
Antiproliferative activity against human H1650 cells assessed as reduction in cell viability after 72 hrs by cell titer glow assay
Antiproliferative activity against human H1650 cells assessed as reduction in cell viability after 72 hrs by cell titer glow assay
[PMID: 32912429]
SK-OV-3 EC50
3 3
Compound: 18
Induction of autophagy in human SKOV3 cells assessed as accumulation of p62 by immunofluorescence assay
Induction of autophagy in human SKOV3 cells assessed as accumulation of p62 by immunofluorescence assay
[PMID: 32912429]
SK-OV-3 EC50
>20 3
Compound: 18
Induction of autophagy in human SKOV3 cells assessed as accumulation of NRB1 by immunofluorescence assay
Induction of autophagy in human SKOV3 cells assessed as accumulation of NRB1 by immunofluorescence assay
[PMID: 32912429]
SK-OV-3 EC50
> 20 3
Compound: 18
Induction of autophagy in human SKOV3 cells assessed as accumulation of NRB1 by immunofluorescence assay
Induction of autophagy in human SKOV3 cells assessed as accumulation of NRB1 by immunofluorescence assay
[PMID: 32912429]
SK-OV-3 EC50
3 3
Compound: 18
Induction of autophagy in human SKOV3 cells assessed as accumulation of p62 by immunofluorescence assay
Induction of autophagy in human SKOV3 cells assessed as accumulation of p62 by immunofluorescence assay
[PMID: 32912429]
SK-OV-3 EC50
3 3
Compound: 18
Induction of autophagy in human SKOV3 cells assessed as accumulation of p62 by immunofluorescence assay
Induction of autophagy in human SKOV3 cells assessed as accumulation of p62 by immunofluorescence assay
[PMID: 32912429]
SK-OV-3 EC50
> 20 3
Compound: 18
Induction of autophagy in human SKOV3 cells assessed as accumulation of NRB1 by immunofluorescence assay
Induction of autophagy in human SKOV3 cells assessed as accumulation of NRB1 by immunofluorescence assay
[PMID: 32912429]
体外実験

ATG7-IN-3 inhibits ATG7 with an IC50 value of 0.048 μM[1].
ATG7-IN-3 inhibits endogenous LC3B puncta formation in H4 cells (glioma cells) with an IC50 of 0.938 μM[1].
ATG7-IN-3 induces p62 accumulation in SKOV-3 cells (p62 EC50 of 3.0 μM)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ATG7-IN-3 関連抗体
体内実験

ATG7-IN-3 (150 mg/kg; s.c.) regulates autophagy markers LC3B and NBR1 in HCT-116 xenografted mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
分子量

376.41

分子式

C11H16N6O5S2
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

CSC1=NN([C@]2([H])O[C@@H]([C@H](C2)O)COS(N)(=O)=O)C3=NC=NC(N)=C31
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (265.67 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.6567 mL 13.2834 mL 26.5668 mL
5 mM 0.5313 mL 2.6567 mL 5.3134 mL
10 mM 0.2657 mL 1.3283 mL 2.6567 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.64 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.64 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.6567 mL 13.2834 mL 26.5668 mL 66.4169 mL
5 mM 0.5313 mL 2.6567 mL 5.3134 mL 13.2834 mL
10 mM 0.2657 mL 1.3283 mL 2.6567 mL 6.6417 mL
15 mM 0.1771 mL 0.8856 mL 1.7711 mL 4.4278 mL
20 mM 0.1328 mL 0.6642 mL 1.3283 mL 3.3208 mL
25 mM 0.1063 mL 0.5313 mL 1.0627 mL 2.6567 mL
30 mM 0.0886 mL 0.4428 mL 0.8856 mL 2.2139 mL
40 mM 0.0664 mL 0.3321 mL 0.6642 mL 1.6604 mL
50 mM 0.0531 mL 0.2657 mL 0.5313 mL 1.3283 mL
60 mM 0.0443 mL 0.2214 mL 0.4428 mL 1.1069 mL
80 mM 0.0332 mL 0.1660 mL 0.3321 mL 0.8302 mL
100 mM 0.0266 mL 0.1328 mL 0.2657 mL 0.6642 mL
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ATG7-IN-3 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

ATG7-IN-32226229-76-9Atg8/LC3Atg7AutophagyAutophagy-related 8| E1 enzymeautophagyH1650 cellsH4 cellsneurogliomaLC3BNBR1Inhibitorinhibitorinhibit

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製品名:
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製品番号:
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