Biotin-epoxomicin 

Biotin-epoxomicin is a Proteasome subunit binder. Biotin-epoxomicin serves as an affinity reagent for identifying proteasomal catalytic subunits, and facilitates affinity purification of active proteasomal subunits for LC-MS identification. Biotin-epoxomicin can be used to study solid tumors derived from B16 melanoma^[1][2].

Biotin-epoxomicin (10 μM; 1 hr) covalently binds to the catalytically active β5t subunit in young and adult murine thymus homogenates, enabling affinity purification and definitive LC-MS³ identification of the β5t active-site peptide^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

724.95

C₃₅H₆₀N₆O₈S

1334608-28-4

Solid

White to off-white

Biotin-IIT-{Leu((2R)-2-methyloxiranyl)}

Biotin-Ile-Ile-Thr-{Leu((2R)-2-methyloxiranyl)}

Room temperature in continental US; may vary elsewhere.

Sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

• [1]. Sin N, et al. Total synthesis of the potent proteasome inhibitor epoxomicin: a useful tool for understanding proteasome biology. Bioorg Med Chem Lett. 1999;9(15):2283-2288.  [Content Brief]

  [2]. Florea BI, et al. Activity-based profiling reveals reactivity of the murine thymoproteasome-specific subunit beta5t. Chem Biol. 2010;17(8):795-801.  [Content Brief]