2. Cell Cycle/DNA Damage Apoptosis
  3. DNA/RNA Synthesis Apoptosis
  4. CHD1Li 6.11

CHD1Li 6.11 is a potent and orally active Chromodomain Helicase DNA Binding Protein 1 Like (CHD1L) (oncogenic gene) inhibitor (IC50 = 3.3 µM for cat-CHD1L recombinant protein). CHD1Li 6.11 can inhibit EMT, induce mesenchymal-epithelial transition (reverse EMT) and promote apoptosis in tumor organoid models. CHD1Li 6.11 can be used for the research of cancer, such as colorectal cancer.

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CHD1Li 6.11

CHD1Li 6.11 構造式

CAS 番号 : 2716890-91-2

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 291 在庫あり
Solution
10 mM * 1 mL in DMSO USD 291 在庫あり
Solid
5 mg $280 在庫あり
10 mg $450 在庫あり
25 mg $900 在庫あり
50 mg $1350 在庫あり
100 mg $2025 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

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CHD1Li 6.11 関連抗体

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DNA/RNA Synthesis アイソフォーム固有の製品をすべて表示:

すべてのアイソフォームを表示
RNASE L DNA Polymerases RNA Polymerases Helicases DNA Ligase

  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

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製品説明

CHD1Li 6.11 is a potent and orally active Chromodomain Helicase DNA Binding Protein 1 Like (CHD1L) (oncogenic gene) inhibitor (IC50 = 3.3 µM for cat-CHD1L recombinant protein). CHD1Li 6.11 can inhibit EMT, induce mesenchymal-epithelial transition (reverse EMT) and promote apoptosis in tumor organoid models. CHD1Li 6.11 can be used for the research of cancer, such as colorectal cancer[1].

IC50 & Target

Helicase

 

Cellular Effect
Cell Line Type Value Description References
HCT-116 IC50
0.8 μM
Compound: 6.11
Inhibition of cell stemness in human HCT-116 M-phenotype cells assessed as reduction in colony formation
Inhibition of cell stemness in human HCT-116 M-phenotype cells assessed as reduction in colony formation
[PMID: 35192363]
HCT-116 IC50
4.3 μM
Compound: 6.11
Cytotoxicity against human HCT-116 M-phenotype tumor organoid assessed as reduction in cell viability incubated for 72 hrs by CellTiter Glo assay
Cytotoxicity against human HCT-116 M-phenotype tumor organoid assessed as reduction in cell viability incubated for 72 hrs by CellTiter Glo assay
[PMID: 35192363]
HCT-116 IC50
5 μM
Compound: 6.11
Induction of mesenchymal-epithelial transition in human HCT-116 M-phenotype tumor organoid assessed as downregulation of vimentin expression by fluorescence analysis
Induction of mesenchymal-epithelial transition in human HCT-116 M-phenotype tumor organoid assessed as downregulation of vimentin expression by fluorescence analysis
[PMID: 35192363]
SW-620 IC50
1.6 μM
Compound: 6.11
Inhibition of cell stemness in human SW-620 M-phenotype cells assessed as reduction in colony formation
Inhibition of cell stemness in human SW-620 M-phenotype cells assessed as reduction in colony formation
[PMID: 35192363]
SW-620 IC50
2.6 μM
Compound: 6.11
Cytotoxicity against human SW-620 M-phenotype tumor organoid assessed as reduction in cell viability incubated for 72 hrs by CellTiter Glo assay
Cytotoxicity against human SW-620 M-phenotype tumor organoid assessed as reduction in cell viability incubated for 72 hrs by CellTiter Glo assay
[PMID: 35192363]
SW-620 IC50
2.6 μM
Compound: 6.11
Cytotoxicity against human SW-620 tumor organoid assessed as reduction in cell viability incubated for 72 hrs by CellTiter Glo assay
Cytotoxicity against human SW-620 tumor organoid assessed as reduction in cell viability incubated for 72 hrs by CellTiter Glo assay
[PMID: 35192363]
SW-620 IC50
3.3 μM
Compound: 6.11
Induction of mesenchymal-epithelial transition in human SW-620 M-phenotype tumor organoid assessed as downregulation of vimentin expression by fluorescence analysis
Induction of mesenchymal-epithelial transition in human SW-620 M-phenotype tumor organoid assessed as downregulation of vimentin expression by fluorescence analysis
[PMID: 35192363]
体外実験

CHD1Li 6.11 shows an IC50 of 2.6 μM against SW620 tumor organoid[1].
CHD1Li 6.11 (1-100 μM, 24 h) reduces TCF-transcription in M-Phenotype SW620 and HCT116 cells[1].
CHD1Li 6.11 (1-100 μM, 72 h) reduces viabilities in M-Phenotype SW620 and HCT116 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

CHD1Li 6.11 関連抗体
体内実験

CHD1Li 6.11 (75-125 mg/kg, p.o., 5 times a week for 28 days) inhibits tumor growth in athymic nude mouse xenografts baring SW620 isolated M-Phenotype flank tumors[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Athymic nude mouse xenografts baring SW620 isolated M-Phenotype flank tumors[1]
Dosage: 75 and 125 mg/kg
Administration: Orally administration, 5 times a week for 28 days
Result: Reduced tumor volume.
Had no abnormal observations associated with gross toxicity.
分子量

472.40

分子式

C21H22BrN5OS
CAS 番号
Appearance

Solid

Color

Off-white to gray

SMILES

O=C(NC1=CC=C(NC2=NC(N3CCCC3)=NC(C)=C2)C=C1)CC4=CC(Br)=CS4
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (211.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1169 mL 10.5843 mL 21.1685 mL
5 mM 0.4234 mL 2.1169 mL 4.2337 mL
10 mM 0.2117 mL 1.0584 mL 2.1169 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.29 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1169 mL 10.5843 mL 21.1685 mL 52.9213 mL
5 mM 0.4234 mL 2.1169 mL 4.2337 mL 10.5843 mL
10 mM 0.2117 mL 1.0584 mL 2.1169 mL 5.2921 mL
15 mM 0.1411 mL 0.7056 mL 1.4112 mL 3.5281 mL
20 mM 0.1058 mL 0.5292 mL 1.0584 mL 2.6461 mL
25 mM 0.0847 mL 0.4234 mL 0.8467 mL 2.1169 mL
30 mM 0.0706 mL 0.3528 mL 0.7056 mL 1.7640 mL
40 mM 0.0529 mL 0.2646 mL 0.5292 mL 1.3230 mL
50 mM 0.0423 mL 0.2117 mL 0.4234 mL 1.0584 mL
60 mM 0.0353 mL 0.1764 mL 0.3528 mL 0.8820 mL
80 mM 0.0265 mL 0.1323 mL 0.2646 mL 0.6615 mL
100 mM 0.0212 mL 0.1058 mL 0.2117 mL 0.5292 mL
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CHD1Li 6.11 Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

CHD1Li 6.112716890-91-2DNA/RNA SynthesisApoptosisDNACHD1LEMTColorectal cancerSW620Inhibitorinhibitorinhibit

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製品名:
CHD1Li 6.11
製品番号:
HY-144256
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