D-Ala-Lys-AMCA 

D-Ala-Lys-AMCA is a known proton-coupled oligopeptide transporter 1 (PEPT1) substrate that emits blue fluorescence. D-Ala-Lys-AMCA may be transported into liver cancer cells and Caco-2 cells based on fluorescence analysis. D-Ala-Lys-AMCA can be used for characterizing PEPT1-specific substrates or inhibitors (Ex/Em = 390/480 nm)^[1][2].

D-Ala-Lys-AMCA (25-150 μM, 3 h) may be transported into liver cancer and Caco-2 cells^[1].
D-Ala-Lys-AMCA (25 μM, 10 min) can be uptaken by absorptive enterocytes of all small intestine segments, whereas there is a complete lack of fluorescence in colonic samples^[2].
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)^[1].
1. Preparation of stock solution:
Use ddH2O or DMSO to prepare a D-Ala-Lys-AMCA stock solution.
2. Preparation of working solution:
Use preheated serum-free cell culture medium or PBS to dilute the stock solution to prepare a 1 mM D-Ala-Lys-AMCA working solution.
Note: Please adjust the concentration of D-Ala-Lys-AMCA working solution according to actual conditions and prepare it before use.
3. Cell staining:
3.1 Add PBS to wash the cells twice, 5 minutes each time.
3.2 Add D-Ala-Lys-AMCA working solution and incubate at 37°C for 1-2 h.
3.3 Aspirate the dye working solution, wash 2-3 times with culture medium, 5 minutes each time, and observe using a fluorescence microscope (Ex/Em = 390/480 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

432.47

C₂₁H₂₈N₄O₆

375822-19-8

480

390

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Gong Y, et al. Specific expression of proton-coupled oligopeptide transporter 1 in primary hepatocarcinoma-anovel strategy for tumor-targeted therapy. Oncol Lett. 2017 Oct;14(4):4158-4166.  [Content Brief]

  [2]. Groneberg DA, et al. Intestinal peptide transport: ex vivo uptake studies and localization of peptide carrier PEPT1. Am J Physiol Gastrointest Liver Physiol. 2001 Sep;281(3):G697-704.  [Content Brief]