2. Apoptosis
  3. Apoptosis
  4. DC_AC50

DC_AC50 is a dual inhibitor of Atox1 and CCS (copper chaperones). Inhibiting intracellular copper chaperones as a means of reducing/preventing acquired chemotherapy resistance.

For research use only. We do not sell to patients.

DC_AC50 Chemical Structure

DC_AC50 Chemical Structure

CAS No. : 497061-48-0

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Solid + Solvent
10 mM * 1 mL in DMSO
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10 mg USD 130 In-stock
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Based on 1 publication(s) in Google Scholar

DC_AC50 Related Antibodies

Top Publications Citing Use of Products

1 Publications Citing Use of MCE DC_AC50

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

DC_AC50 is a dual inhibitor of Atox1 and CCS (copper chaperones). Inhibiting intracellular copper chaperones as a means of reducing/preventing acquired chemotherapy resistance[1].
In Vitro

DC_AC50 exhibits IC50 values of 9.88 μM, 12.57 μM, 5.96 μM and 6.68 μM in Canine Abrams, Canine D1, human HOS and human MG63) cells, respectively[1].
? DC_AC50 (0-10 μM)-treated cells are significantly less mitotically active, as demonstrated by decreased expression of phospho-histone H3 and cell cycle analysis[1].
? DC_AC50 (10 μM) potentiates carboplatin-induced apoptosis in OSA cells and decreasesclonogenic survival[1].
? DC_AC50 induces cell cycle arrest at both the 3 and 10 μM doses and? DC_AC50 induces increase S phase cells dose-independently[1].
? DC_AC50 (3 μM) inhibits the migration and of canine and human OSA cells[1].
? DC_AC50 (2.5-10 μM) is highly efficient at inhibiting cancer cell proliferation (human lung cancer H1299 cells, leukaemia cancer K562 cells, breast cancer MDA-MB-231 cells and head and neck cancer 212LN cells) in a dose-dependent manner. DC_AC50 fails to exhibit any notable inhibition of the cell proliferation of human normal epithelial lung BEAS-2B cells or breast MCF-10A cells as control cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DC_AC50 Related Antibodies

Cell Viability Assay[1]

Cell Line: Canine OSA (Abrams, D1 and human OSA (HOS, MG63) cells.
Concentration: 0-10 μM.
Incubation Time: 72 h.
Result: Dose-dependently decreased viability of OSA cells.

Apoptosis Analysis[1]

Cell Line: Abrams and HOS cells.
Concentration: 1, 3 and 10 μM (10 μM Carboplatin).
Incubation Time: 24 h.
Result: Potentiated carboplatin-induced apoptosis.
Molecular Weight

424.26

Formula

C17H12BrF2N3OS
CAS No.
Appearance

Solid

Color

White to off-white

SMILES

NC1=C(SC2=C1C=C3CCCC3=N2)C(NC4=C(Br)C=C(C=C4F)F)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (117.85 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.3570 mL 11.7852 mL 23.5705 mL
5 mM 0.4714 mL 2.3570 mL 4.7141 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (5.89 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (5.89 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.97%

COA (249 KB) HNMR (265 KB) RP-HPLC (205 KB) MS (176 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.3570 mL 11.7852 mL 23.5705 mL 58.9261 mL
5 mM 0.4714 mL 2.3570 mL 4.7141 mL 11.7852 mL
10 mM 0.2357 mL 1.1785 mL 2.3570 mL 5.8926 mL
15 mM 0.1571 mL 0.7857 mL 1.5714 mL 3.9284 mL
20 mM 0.1179 mL 0.5893 mL 1.1785 mL 2.9463 mL
25 mM 0.0943 mL 0.4714 mL 0.9428 mL 2.3570 mL
30 mM 0.0786 mL 0.3928 mL 0.7857 mL 1.9642 mL
40 mM 0.0589 mL 0.2946 mL 0.5893 mL 1.4732 mL
50 mM 0.0471 mL 0.2357 mL 0.4714 mL 1.1785 mL
60 mM 0.0393 mL 0.1964 mL 0.3928 mL 0.9821 mL
80 mM 0.0295 mL 0.1473 mL 0.2946 mL 0.7366 mL
100 mM 0.0236 mL 0.1179 mL 0.2357 mL 0.5893 mL
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DC_AC50 Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

DC_AC50497061-48-0ApoptosisChemotherapyresistanceAtox1CCScopperchaperonesosteosarcomaInhibitorinhibitorinhibit

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