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  6. (-)-Epigallocatechin-3-(3''-O-methyl) gallate

(-)-Epigallocatechin-3-(3''-O-methyl) gallate  (Synonyms: (-)-EGCG-3''-O-ME)

製品番号: HY-N2228 純度: 99.83%
COA 取扱説明書 Technical Support

(-)-Epigallocatechin-3-(3''-O-methyl) gallate ((-)-EGCG-3''-O-ME) is a natural product that can be isolated from the tea leaf, with strong antioxidative activity. (-)-Epigallocatechin-3-(3''-O-methyl) gallate has a strong cytotoxic activity for rat cancer cells.

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(-)-Epigallocatechin-3-(3''-O-methyl) gallate

(-)-Epigallocatechin-3-(3''-O-methyl) gallate 構造式

CAS 番号 : 83104-87-4

容量 価格（税別） 在庫状況 数量
1 mg $200 在庫あり
5 mg $480 在庫あり
10 mg $650 在庫あり
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100 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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(-)-Epigallocatechin-3-(3''-O-methyl) gallate 関連抗体

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製品説明

(-)-Epigallocatechin-3-(3''-O-methyl) gallate ((-)-EGCG-3''-O-ME) is a natural product that can be isolated from the tea leaf, with strong antioxidative activity. (-)-Epigallocatechin-3-(3''-O-methyl) gallate has a strong cytotoxic activity for rat cancer cells[1].

体外実験

(-)-Epigallocatechin-3-(3''-O-methyl) gallate scavenges DPPH-derived radicals (IC50: 36.54 μM), and scavenges ABTS radicals (IC50: 2.59 μM)[2].
(-)-Epigallocatechin-3-(3''-O-methyl) gallate (12.5 μM, 30 min) scavenges intracellular ROS production in RAW264.7 cells[2].
(-)-Epigallocatechin-3-(3''-O-methyl) gallate (6.25 and 12 μM, 24-72 h) promotes cell proliferation in HaCaT cells[2].
(-)-Epigallocatechin-3-(3''-O-methyl) gallate (6.25 and 12 μM, 12 h) increased HO-1 expression in HaCaT cells treated with 250 μM H2O2[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

(-)-Epigallocatechin-3-(3''-O-methyl) gallate 関連抗体

Western Blot Analysis[2]

Cell Line: HaCaT cells treated with 250 μM H2O2
Concentration: 6.25 and 12 μM
Incubation Time: 24 h
Result: Increased HO-1 expression, indicating the cytoprotective role against H2O2.
分子量

472.40

分子式

C23H20O11
CAS 番号
Appearance

Solid

Color

Off-white to pink

SMILES

O=C(O[C@H]1[C@@H](C2=CC(O)=C(O)C(O)=C2)OC3=CC(O)=CC(O)=C3C1)C4=CC(OC)=C(O)C(O)=C4
Structure Classification
Initial Source
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
体外: 

DMSO : 50 mg/mL (105.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1169 mL 10.5843 mL 21.1685 mL
5 mM 0.4234 mL 2.1169 mL 4.2337 mL
10 mM 0.2117 mL 1.0584 mL 2.1169 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Volume
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

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体積 （開始）

V1

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体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 1.25 mg/mL (2.65 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 1.25 mg/mL (2.65 mM); Clear solution

    This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.83%

COA (256 KB) HNMR (227 KB) CNMR (236 KB) RP-HPLC (230 KB) MS (117 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.1169 mL 10.5843 mL 21.1685 mL 52.9213 mL
5 mM 0.4234 mL 2.1169 mL 4.2337 mL 10.5843 mL
10 mM 0.2117 mL 1.0584 mL 2.1169 mL 5.2921 mL
15 mM 0.1411 mL 0.7056 mL 1.4112 mL 3.5281 mL
20 mM 0.1058 mL 0.5292 mL 1.0584 mL 2.6461 mL
25 mM 0.0847 mL 0.4234 mL 0.8467 mL 2.1169 mL
30 mM 0.0706 mL 0.3528 mL 0.7056 mL 1.7640 mL
40 mM 0.0529 mL 0.2646 mL 0.5292 mL 1.3230 mL
50 mM 0.0423 mL 0.2117 mL 0.4234 mL 1.0584 mL
60 mM 0.0353 mL 0.1764 mL 0.3528 mL 0.8820 mL
80 mM 0.0265 mL 0.1323 mL 0.2646 mL 0.6615 mL
100 mM 0.0212 mL 0.1058 mL 0.2117 mL 0.5292 mL
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(-)-Epigallocatechin-3-(3''-O-methyl) gallate Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

(-)-Epigallocatechin-3-(3''-O-methyl) gallate83104-87-4(-)-EGCG-3''-O-MEOthersantioxidativeanticancerHaCaTRAW264.7Inhibitorinhibitorinhibit

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製品名:
(-)-Epigallocatechin-3-(3''-O-methyl) gallate
製品番号:
HY-N2228
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