2. Anti-infection
  3. Enterovirus
  4. Golgicide A

Golgicide A (GCA) is a potent, highly specific, and reversible inhibitor of the cis-Golgi ADP-ribosylation factor guanine nucleotide exchange factors (ArfGEF) GBF1. Golgicide A drastically reduced replication of coxsackievirus B3 (CVB3) and other human enterovirus species.

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CAS 番号 : 1139889-93-2

容量 価格（税別） 在庫状況 数量
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 79 在庫あり
Solution
10 mM * 1 mL in DMSO USD 79 在庫あり
Solid
5 mg $72 在庫あり
10 mg $127 在庫あり
25 mg $250 在庫あり
50 mg $400 在庫あり
100 mg $640 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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カスタマーレビュー

Based on 11 publication(s) in Google Scholar

Other Forms of Golgicide A:

Golgicide A 関連抗体

Top Publications Citing Use of Products

    Golgicide A purchased from MedChemExpress. Usage Cited in: J Virol. 2024 Jun 21:e0081324.  [Abstract]

    Expression of BiP and XBP1s in Golgicide A (GCA)-treated RD cells (1, 3, 10 µM) for 24 h was quantified using qPCR.

    Golgicide A purchased from MedChemExpress. Usage Cited in: J Virol. 2024 Jun 21:e0081324.  [Abstract]

    RD cells expressing ARF1 Q71L at varying levels were treated with Golgicide A (GCA) (10 µM) 24 h post-transfection. The expression of ARF1 was detected using immunoblotting at 48 h post-transfection.

    Golgicide A purchased from MedChemExpress. Usage Cited in: Mol Metab. 2021 Dec;54:101329.  [Abstract]

    Intracellular insulin and proinsulin protein levels after 30 h loperamide treatment +/− 10 μM Golgicide A for the final 6 h, as measured by ELISA.

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Golgicide A (GCA) is a potent, highly specific, and reversible inhibitor of the cis-Golgi ADP-ribosylation factor guanine nucleotide exchange factors (ArfGEF) GBF1[1]. Golgicide A drastically reduced replication of coxsackievirus B3 (CVB3) and other human enterovirus species[2].

    IC50 & Target

    GBF1[1]
    Cellular Effect
    Cell Line Type Value Description References
    Vero IC50
    3.3 3
    Compound: 2, GCA
    Restoration of protein synthesis in african green monkey Vero cells assessed as radioactive aminoacid incorporation after 30 min in presence of shiga toxin
    Restoration of protein synthesis in african green monkey Vero cells assessed as radioactive aminoacid incorporation after 30 min in presence of shiga toxin
    		[PMID: 19182783]
    Vero IC50
    3.3 3
    Compound: 2, GCA
    Restoration of protein synthesis in african green monkey Vero cells assessed as radioactive aminoacid incorporation after 30 min in presence of shiga toxin
    Restoration of protein synthesis in african green monkey Vero cells assessed as radioactive aminoacid incorporation after 30 min in presence of shiga toxin
    		[PMID: 19182783]
    Vero IC50
    3.3 3
    Compound: 2, GCA
    Restoration of protein synthesis in african green monkey Vero cells assessed as radioactive aminoacid incorporation after 30 min in presence of shiga toxin
    Restoration of protein synthesis in african green monkey Vero cells assessed as radioactive aminoacid incorporation after 30 min in presence of shiga toxin
    		[PMID: 19182783]
    体外実験

    Golgicide A (GCA) is a potent and highly effective inhibitor of shiga toxin activity. Golgicide A (GCA) inhibits the effect of shiga toxin on protein synthesis with an IC50 of 3.3 μM[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Golgicide A 関連抗体
    臨床実験
    分子量

    284.30

    分子式

    C17H14F2N2
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    FC1=CC2=C(NC(C3=CC=CN=C3)[C@@]4([H])[C@]2([H])C=CC4)C(F)=C1
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 100 mg/mL (351.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.5174 mL 17.5871 mL 35.1741 mL
    5 mM 0.7035 mL 3.5174 mL 7.0348 mL
    10 mM 0.3517 mL 1.7587 mL 3.5174 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

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    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.5 mg/mL (8.79 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% Corn Oil

      Solubility: ≥ 2.5 mg/mL (8.79 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.94%

    COA (252 KB) HNMR (143 KB) RP-HPLC (162 KB) LCMS (236 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.5174 mL 17.5871 mL 35.1741 mL 87.9353 mL
    5 mM 0.7035 mL 3.5174 mL 7.0348 mL 17.5871 mL
    10 mM 0.3517 mL 1.7587 mL 3.5174 mL 8.7935 mL
    15 mM 0.2345 mL 1.1725 mL 2.3449 mL 5.8624 mL
    20 mM 0.1759 mL 0.8794 mL 1.7587 mL 4.3968 mL
    25 mM 0.1407 mL 0.7035 mL 1.4070 mL 3.5174 mL
    30 mM 0.1172 mL 0.5862 mL 1.1725 mL 2.9312 mL
    40 mM 0.0879 mL 0.4397 mL 0.8794 mL 2.1984 mL
    50 mM 0.0703 mL 0.3517 mL 0.7035 mL 1.7587 mL
    60 mM 0.0586 mL 0.2931 mL 0.5862 mL 1.4656 mL
    80 mM 0.0440 mL 0.2198 mL 0.4397 mL 1.0992 mL
    100 mM 0.0352 mL 0.1759 mL 0.3517 mL 0.8794 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Golgicide A Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Golgicide A1139889-93-2GCAEnterovirusRhinovirusHRVHRVsHEVHEVsInhibitorinhibitorinhibit

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    製品名:
    Golgicide A
    製品番号:
    HY-100540
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