Heme Oxygenase-1-IN-1 hydrochloride

Name: Heme Oxygenase-1-IN-1 hydrochloride
Brand: MedChemExpress
SKU: HY-111798A
Rating: 5.0 (11 reviews)

Cat. No.: HY-111798A Pureza: 99.03%: Ficha de datos
COA

SDS
Instrucciones de manejo
FAQs
Technical Support

Heme Oxygenase-1-IN-1 (Compound 2) hydrochloride is a heme oxygenase 1 (HO-1) inhibitor with an IC₅₀ of 0.25 μM.

Para uso exclusivo en investigación. No vendemos a pacientes.

Heme Oxygenase-1-IN-1 hydrochloride Estructura química

No. CAS : 1092851-70-1

Tamaño	Stock	Cantidad

Muestra gratis (0.1 - 0.2 mg)	Solicite ahora
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Solid
5 mg	En stock	Estimated Time of Arrival: December 31
10 mg	En stock	Estimated Time of Arrival: December 31
25 mg	En stock	Estimated Time of Arrival: December 31
50 mg	En stock	Estimated Time of Arrival: December 31
100 mg	En stock	Estimated Time of Arrival: December 31
200 mg	Obtener un presupuesto
500 mg	Obtener un presupuesto

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Revisión del cliente

Based on 11 publication(s) in Google Scholar

Other Forms of Heme Oxygenase-1-IN-1 hydrochloride:

Heme Oxygenase-1-IN-1 In-stock

11 Publications Citing Use of MCE Heme Oxygenase-1-IN-1 hydrochloride

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Actividad biológica
Pureza y Documentación
Referencias
Revisión del cliente

Descripciòn

Heme Oxygenase-1-IN-1 (Compound 2) hydrochloride is a heme oxygenase 1 (HO-1) inhibitor with an IC₅₀ of 0.25 μM^[1].

IC₅₀ & Target

IC₅₀: 0.25 μM (HO-1)^[1]

In Vitro

Heme Oxygenase-1-IN-1 hydrochloride (0-10 µM) attenuates Dipeptidyl peptidase-4 inhibitors (DPP-4i)-induced NF-кB activation in 4T1 cells^[2].
Heme Oxygenase-1-IN-1 hydrochloride (0-10 µM) significantly decreases GC cell migration and invasion in parental gastric cancer cells^[3].
Heme Oxygenase-1-IN-1 hydrochloride significantly down-regulates HO-1 mRNA level and metastasis-associated gene expressions in GRIM-19-deficient gastric cancer cells^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Peso molecular

315.64

Fòrmula

C₁₃H₁₆BrClN₂

No. CAS

1092851-70-1

Appearance

Solid

Color

Light yellow to yellow

SMILES

BrC1=CC=C(CCCCN2C=CN=C2)C=C1.Cl

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvente y solubilidad

In Vitro:

DMSO : 125 mg/mL (396.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 100 mg/mL (316.82 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1682 mL	15.8408 mL	31.6817 mL
5 mM	0.6336 mL	3.1682 mL	6.3363 mL
10 mM	0.3168 mL	1.5841 mL	3.1682 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (6.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (6.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (6.59 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 100 mg/mL (316.82 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Pureza y Documentación

Purity: 99.03%

Select Batch:

Ficha de datos (273 KB) SDS (252 KB)

COA (245 KB) LCMS (95 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Floresta G, et al. Development of new HO-1 inhibitors by a thorough scaffold-hopping analysis. Bioorg Chem. 2018 Dec;81:334-339. [Content Brief]

[2]. Zeng X, et al. Mitochondrial GRIM-19 loss in parietal cells promotes spasmolytic polypeptide-expressing metaplasia through NLR family pyrin domain-containing 3 (NLRP3)-mediated IL-33 activation via a reactive oxygen species (ROS) -NRF2- Heme oxygenase-1(HO-1)-NF-кB axis. Free Radic Biol Med. 2023 Jun;202:46-61. [Content Brief]

[3]. Wang X, et al. Mitochondrial GRIM-19 deficiency facilitates gastric cancer metastasis through oncogenic ROS-NRF2-HO-1 axis via a NRF2-HO-1 loop. Gastric Cancer. 2021 Jan;24(1):117-132. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	3.1682 mL	15.8408 mL	31.6817 mL	79.2042 mL
	5 mM	0.6336 mL	3.1682 mL	6.3363 mL	15.8408 mL
	10 mM	0.3168 mL	1.5841 mL	3.1682 mL	7.9204 mL
	15 mM	0.2112 mL	1.0561 mL	2.1121 mL	5.2803 mL
	20 mM	0.1584 mL	0.7920 mL	1.5841 mL	3.9602 mL
	25 mM	0.1267 mL	0.6336 mL	1.2673 mL	3.1682 mL
	30 mM	0.1056 mL	0.5280 mL	1.0561 mL	2.6401 mL
	40 mM	0.0792 mL	0.3960 mL	0.7920 mL	1.9801 mL
	50 mM	0.0634 mL	0.3168 mL	0.6336 mL	1.5841 mL
	60 mM	0.0528 mL	0.2640 mL	0.5280 mL	1.3201 mL
	80 mM	0.0396 mL	0.1980 mL	0.3960 mL	0.9901 mL
	100 mM	0.0317 mL	0.1584 mL	0.3168 mL	0.7920 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.