Insulin-Transferrin-Selenium, 100X  (Synonyms: ITS-G)

Insulin-Transferrin-Selenium, 100X (ITS-G) is a mixture of Insulin, transferrin, and sodium selenite that is a general supplement designed for use in classic media such as DMEM, RPMI-1640, and nutrient media such as Ham's F-12, DMEM/F-12. Insulin-Transferrin-Selenium is added to basal medium to reduce the amount of fetal calf serum required to culture cells^[1].

Insulin-Transferrin-Selenium, 100X (1%) can be supplement for DMEM/F12 medium together with 10% FBS, 1% Penicillin/Streptomycin (HY-B1906) and 40 ng/mL Dexamethasone (HY-14648) to culture AML-12 hepatocyte cells^[1].
Insulin-Transferrin-Selenium, 100X (4 mL/L) is added to supplement F12 medium together with 5 g/L D-glucose (HY-B0389), 10 mM HEPES (HY-D0857), 7.5 mM L-glutamine (HY-N0390), and 1% FBS to culture HEK293 EBNA cells and CHO-s cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Liquid

Colorless to light yellow

[Insulin-Transferrin-Selenium, 100X]

Room temperature in continental US; may vary elsewhere.

Solution, -20°C, 2 years

• [1]. Wei, M., et al., (2022). Hepatocyte-Specific Smad4 Deficiency Alleviates Liver Fibrosis via the p38/p65 Pathway. International journal of molecular sciences, 23(19), 11696.  [Content Brief]

  [2]. Croset, A., et al., (2012). Differences in the glycosylation of recombinant proteins expressed in HEK and CHO cells. Journal of biotechnology, 161(3), 336–348.  [Content Brief]