2. Metabolic Enzyme/Protease
  3. Glycosidase
  4. Kifunensine

Kifunensine  (Synonyms: キフネンシン; FR 900494)

製品番号: HY-19332 純度: 99.90%
COA 取扱説明書 Technical Support

Kifunensine, a potent and selective inhibitor of class I α-mannosidases isolated from Actinomycete, prevents α-mannosidases I from trimming mannose residues on glycoproteins. Kifunensine inhibits ERAD.

商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。

CAS 番号 : 109944-15-2

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 USD 198 在庫あり
Solution
10 mM * 1 mL in DMSO USD 198 在庫あり
Solid
5 mg $180 在庫あり
10 mg $280 在庫あり
25 mg $590 在庫あり
50 mg $975 在庫あり
100 mg $1650 在庫あり
200 mg   お問い合わせ  
500 mg   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

This product is a controlled substance and not for sale in your territory.

カスタマーレビュー

Based on 13 publication(s) in Google Scholar

Other Forms of Kifunensine:

Kifunensine 関連抗体

Top Publications Citing Use of Products

    Kifunensine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jan 14:e2409105.  [Abstract]

    Western blot analysis of BACE1 and Nicastrin expression was performed on CHO-APP cells treated with or without mannose (25 mM), with or without Kifunensine (10 μM).

    Kifunensine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jan 14:e2409105.  [Abstract]

    Immunofluorescence staining of Aβ in cerebrum from 6‐month‐old 5×FAD mice and 5×FAD+Kif (5 mg/kg, daily, iv, 7 days) mice.

    Kifunensine purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jan 14:e2409105.  [Abstract]

    Western blot analysis of BACE1 and Nicastrin expression in cortex and hippocampus of 6‐month‐old 5×FAD mice and 5×FAD+Kif (5 mg/kg, daily, iv, 7 days) mice.

    Kifunensine purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2023 Oct;299(10):105211.  [Abstract]

    Western blot analysis of corin proteins in cell lysates from corin-expressing HEK293 cells treated with N-glycosylation inhibitors, including N-linked glycosylation inhibitor-1 (NGI-1), 1-deoxynojirimycin (1-DNJ), kifunensine (KIF, 10 μM, 48 h), and swainsonine (SWA). The activation-cleaved corin protease domain (corin-p) band is shown. Levels of β-tubulin in cell lysates were used to assess protein amounts in each sample.

    Kifunensine purchased from MedChemExpress. Usage Cited in: J Biol Chem. 2023 Oct;299(10):105211.  [Abstract]

    In cell lysates, the ratio of corin-p versus zymogen bands in 1-DNJ-, KIF- (10 μM, 48 h), and SWA-treated samples decreased to 35 ± 3, 35 ± 6, and 62 ± 17% of the vehicle, respectively (n = 3; p = 0.003, 1-DNJ versus the vehicle; p = 0.003, KIF versus the vehicle; p = 0.049, SWA versus the vehicle).

    • 生物活性

    • 純度とドキュメンテーション

    • 参考文献

    • カスタマーレビュー

    製品説明

    Kifunensine, a potent and selective inhibitor of class I α-mannosidases isolated from Actinomycete, prevents α-mannosidases I from trimming mannose residues on glycoproteins. Kifunensine inhibits ERAD[1][2][3].

    体外実験

    Kifunensine, an alkaloid from actinomycete Kitasatosporia kifunense 9482, is the most efficient nhibitor of a-mannosidase I, blocking N-glycan synthesis at an8GlcNAc2 (Man8) or Man9GlcNAc2 (Man9) stage[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Kifunensine 関連抗体

    RT-PCR[3].

    Cell Line: Hybridoma cells expressing a human IgG1 monoclonal Antibody[3].
    Concentration: 2 μg/mL.
    Incubation Time: 4 days.
    Result: Significantly reduced the lentil lectin binding.
    Kifunensine is the most effective among the inhibitors tested in producing antibodies containing oligomannose residues without fucose.
    体内実験
    Animal Model: BALB/c mice[3].
    Dosage: 5 mg/kg.
    Administration: IV via tail vein.
    Result: There was no significant difference in the serum levels of anti-TEM B mAb generated from cells treated with or without kifunensine in the 7-day period.
    分子量

    232.19

    分子式

    C8H12N2O6
    CAS 番号
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    O=C(N1[C@@](N2)([H])[C@@H](O)[C@@H](O)[C@H](O)[C@H]1CO)C2=O
    Structure Classification
    Initial Source

    actinomycete
    輸送条件

    Room temperature in continental US; may vary elsewhere.
    保管条件
    Powder -20°C 3 years
    In solvent -80°C 6 months
      -20°C 1 month
    溶剤 & 溶解度
    体外: 

    DMSO : 10 mg/mL (43.07 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.3068 mL 21.5341 mL 43.0682 mL
    5 mM 0.8614 mL 4.3068 mL 8.6136 mL
    10 mM 0.4307 mL 2.1534 mL 4.3068 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始）

    C1

    ×
    体積 （開始）

    V1

    =
    濃度 （終了）

    C2

    ×
    体積 （終了）

    V2

    体内:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1.19 mg/mL (5.13 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.19 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (11.9 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 1.19 mg/mL (5.13 mM); Clear solution

      This protocol yields a clear solution of ≥ 1.19 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (11.9 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    純度とドキュメンテーション

    純度: 99.90%

    COA (256 KB) HNMR (252 KB) RP-HPLC (242 KB) MS (69 KB) Elemental Analysis Report (114 KB)

    取扱説明書 (2659 KB)
    参考文献

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.3068 mL 21.5341 mL 43.0682 mL 107.6704 mL
    5 mM 0.8614 mL 4.3068 mL 8.6136 mL 21.5341 mL
    10 mM 0.4307 mL 2.1534 mL 4.3068 mL 10.7670 mL
    15 mM 0.2871 mL 1.4356 mL 2.8712 mL 7.1780 mL
    20 mM 0.2153 mL 1.0767 mL 2.1534 mL 5.3835 mL
    25 mM 0.1723 mL 0.8614 mL 1.7227 mL 4.3068 mL
    30 mM 0.1436 mL 0.7178 mL 1.4356 mL 3.5890 mL
    40 mM 0.1077 mL 0.5384 mL 1.0767 mL 2.6918 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    Kifunensine Related Classifications

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    質量   濃度   体積   分子量 *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    一般には略語で表示されます：C1V1 = C2V2

    濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
    × = ×
    C1   V1   C2   V2
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Kifunensine109944-15-2FR-900494FR900494FR 900494GlycosidaseERADER StressInhibitorinhibitorinhibit

    最近チェックした製品:

    オンラインお問い合わせ

    Your information is safe with us. * Required Fields.

    製品名

    		  

    カスタマ需要量 *

    お名前 *

    		 

    タイトル

    メールアドレス *

    		 

    電話番号 *

    デパートメント

    		 

    組纖名 *

    市区町村

    都道府県

    国或いは地域 *

    		      

    必ず会社名を記載ください。個人への返信は行いません。

    備考

    バルクお問い合わせ

    Inquiry Information

    製品名:
    Kifunensine
    製品番号:
    HY-19332
    数量:
    MCE 日本正規代理店:

    カスタマーレビュー

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    製品名

    		  

    Lot #

    お名前 *

    		 

    メールアドレス *

    電話番号 *

    		 

    部門 *

    組纖名 *

    		 

    国或いは地域 *

    備考

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.