2. Dye Reagents

Dye Reagents

A dye is a colored substance that has an affinity to the substrate to which it is being applied.

A new approach is presented for analysis of microplastics in environmental samples, based on selective fluorescent staining using Nile Red (NR), followed by density-based extraction and filtration. The dye adsorbs onto plastic surfaces and renders them fluorescent when irradiated with blue light. Fluorescence emission is detected using simple photography through an orange filter. Image-analysis allows fluorescent particles to be identified and counted. Magnified images can be recorded and tiled to cover the whole filter area, allowing particles down to a few micrometres to be detected. The solvatochromic nature of Nile Red also offers the possibility of plastic categorisation based on surface polarity characteristics of identified particles. This article details the development of this staining method and its initial cross-validation by comparison with infrared (IR) microscopy. Microplastics of different sizes could be detected and counted in marine sediment samples. The fluorescence staining identified the same particles as those found by scanning a filter area with IR-microscopy.

Cyanine dyes can be used to quantify the amount of dsDNA within a sample. The linearity of fluorescence, as function of DNA amount of six dyes, is obtained by measuring the fluorescence intensity at the optimal excitation and emission maxima.

Dye Reagents (3782):

Cat. No. Product Name CAS No. Purity Chemical Structure
  • HY-D2192
    Ag2S-PEG-NH2 (1200 nm)
    Ag2S-PEG-NH2 (1200 nm) is a fluorescent dyes.
    Ag2S-PEG-NH2 (1200 nm)
  • HY-D2195
    AgAuSe-PEG-NH2 (1000 nm)
    AgAuSe-PEG-NH2 (1000 nm) is a fluorescent quantum dot that emits fluorescence in the near-infrared II region, with an emission peak reaching 1000 nm that can be used in bioimaging.
    AgAuSe-PEG-NH2 (1000 nm)
  • HY-D2284
    SGPL1 fluorogenic substrate 1166838-84-1 98.81%
    SGPL1 fluorogenic substrate is the fluorogenic substrate of sphingosine-1-phosphate lyase (SGPL1) that can be used to measure the activity of SGPL1.
    SGPL1 fluorogenic substrate
  • HY-D2287
    CalFluor 555 azide 1798305-99-3 98%
    CalFluor 555 azide is a fluorogenic azide probe. CalFluor 555 azide is not fluorescent until it is reacted with alkynes (Ex=555 nm).
    CalFluor 555 azide
  • HY-D2345
    GZ22-4 99.37%
    GZ22-4 is a near-infrared (NIR) fluorescent probe. GZ22-4 shows high affinity for carbonic anhydrase IX (CAIX), with a Kd of 0.2 nM. GZ22-4 can be used for the research of visualize CAIX-positive tumors.
    GZ22-4
  • HY-D2551
    Cy5-PEG5000-FA
    Cy5-PEG5000-FA is a fluorescently labeled biomolecule consisting of Cy5 (HY-D0821) fluorescent dye, polyethylene glycol (PEG), and Folic acid (FA) (HY-16637). Cy5 is commonly used in applications such as immunolabeling and nucleic acid labeling. The maximum emission wavelength of Cy5 is approximately 670 nm.
    Cy5-PEG5000-FA
  • HY-D2569
    Cy5.5-PEG2000-Biotin
    Cy5.5-PEG2000-Biotin is a polyethylene glycol derivative containing Cy5.5 (HY-D0924) fluorescent dye and polyethylene glycol (PEG) and a Biotin. Biotin can bind with high affinity to streptavidin or avidin. Cy5.5-PEG2000-Biotin plays an important role in the specific capture and detection of biomolecules.
    Cy5.5-PEG2000-Biotin
  • HY-D2795
    FITC-PEG400-COOH
    FITC-PEG400-COOH is a fluorescent dye composed of polyethylene glycol (PEG), FITC (HY-66019) and a COOH. FITC-PEG400-COOH can be used for fluorescent labeling and imaging.
    FITC-PEG400-COOH
  • HY-D2839
    m-PEG10000-PEI-Cy5 99.50%
    m-PEG10000-PEI-Cy5 is a fluorescent dye composed of polyethylene glycol (PEG), Cy5 (HY-D0821) and Polyethyleneimine (PEI). m-PEG10000-PEI-Cy5 can be used for fluorescent labeling and imaging.
    m-PEG10000-PEI-Cy5
  • HY-D2911
    Pantetheine probe PP-3 2596272-25-0 99.17%
    Pantetheine probe PP-3 (Compound PP-3) is an alkynyl group connector probe. Pantetheine probe PP-3 selectively enriches and maps modification sites (e.g., Ser231 on DHRS2). Pantetheine probe PP-3 is promising for research of metabolic disorders (e.g., fatty acid synthesis dysregulation).
    Pantetheine probe PP-3
  • HY-P2065
    Ac-VEID-AMC 219137-97-0 98.60%
    Ac-VEID-AMC is a fluorescent substrate for the determination of caspase-6 and related cysteine protease activities (Ex=340-360 nm, Em=440-460 nm).
    Ac-VEID-AMC
  • HY-P3362
    Ac-IETD-AMC 348079-17-4 98.50%
    Ac-IETD-AMC is a fluorogenic caspase-8/granzyme B substrate containing the acetyl (Ac) moiety. Ac-IETD-AMC is frequently used to measure caspase-8 activity.
    Ac-IETD-AMC
  • HY-100512
    Benzenepentacarboxylic Acid 1585-40-6 98.39%
    Benzenepentacarboxylic acid is a fluorescent dye that detects and scavenge HO radicals.
    Benzenepentacarboxylic Acid
  • HY-101889
    Cresyl Violet perchlorate 41830-80-2 98%
    Cresyl Violet perchlorate is a red fluorescent stain, which can be used to stain neurons.
    Cresyl Violet perchlorate
  • HY-103373
    PE154 1192750-33-6 99.01%
    PE154 (Compound 13) is a potent fluorescent inhibitor of human acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) (IC50s=280 pM and 16 nM, respectively). PE154 can label β-amyloid plaques in histochemical analysis.
    PE154
  • HY-104056
    Fura Red 149732-62-7 98%
    Fura Red is a Ca2+-sensitive fluorescent dye, which decreases in fluorescence with rising [Ca2+].
    Fura Red
  • HY-107864
    Erythrosine B free acid 15905-32-5 99.07%
    Erythrosine B free acid is a visibly red dye with colorimetric and fluorescent properties that serves as an important dye for many Gram-positive and -negative bacteria. Erythrosine B free acid can be used for live/dead determination in both colorimetric and fluorescence-based assays for low, medium and high-throughput experimentation.
    Erythrosine B free acid
  • HY-111956
    D-Ala-Lys-AMCA 375822-19-8 98%
    D-Ala-Lys-AMCA is a known proton-coupled oligopeptide transporter 1 (PEPT1) substrate that emits blue fluorescence. D-Ala-Lys-AMCA may be transported into liver cancer cells and Caco-2 cells based on fluorescence analysis. D-Ala-Lys-AMCA can be used for characterizing PEPT1-specific substrates or inhibitors (Ex/Em = 390/480 nm).
    D-Ala-Lys-AMCA
  • HY-118320
    Alizarine Yellow R 2243-76-7 98.0%
    Alizarine Yellow R (Mordant orange 1), Salicylic acid derivative (HY-B0167), is an azo dye. Alizarine Yellow R is used as a pH indicator and a biological stain in chemical examinations and dyeing industries.
    Alizarine Yellow R
  • HY-128536
    KMG-104AM 2436544-28-2 98%
    KMG-104AM is a Mg2+ fluorescent probe. KMG-104AM is a membrane-permeable ester-modified derivative of KMG-104, which serves as a reporter and imaging agent. KMG-104AM can track the increase in intracellular free magnesium ion concentration induced by mitochondrial uncoupling. KMG-104AM enables visualization of the three-dimensional distribution of intracellular magnesium ion concentration. KMG-104AM is applicable to research related to the functions of intracellular magnesium ions.
    KMG-104AM