Ac-VEID-AMC
Based on 1 Customer Validation
Ac-VEID-AMC is a fluorescent substrate for the determination of caspase-6 and related cysteine protease activities (Ex=340-360 nm, Em=440-460 nm).
For research use only. We do not sell to patients.
- Purity: 98.60%
- CAS No.: 219137-97-0
- Formula: C32H43N5O11
- Molecular Weight:673.71
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Storage:
Sealed storage, away from moisture.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Enzyme activity (rabbit osteoclasts for example)[1]:
1. Treat purified, differentiated cells by medium for 48 h (time depends on the cell).
2. Put treated cells into 4.5 mL lysis buffer.
3. Each lysate is divided into thirds, then incubate with 40 μM Ac-VEID-AMC at 37°C for 1 h.
4. Detect cleavage of fluorescent substrates (Ex=340-360 nm, Em=440-460 nm)
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
460
380
Chemical Information
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CAS No. 219137-97-0
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Appearance Solid
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Molecular Weight 673.71
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Formula C32H43N5O11
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Color White to off-white
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 25 mg/mL (37.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.4843 mL | 7.4216 mL | 14.8432 mL | 37.1080 mL |
| 5 mM | 0.2969 mL | 1.4843 mL | 2.9686 mL | 7.4216 mL | |
| 10 mM | 0.1484 mL | 0.7422 mL | 1.4843 mL | 3.7108 mL | |
| 15 mM | 0.0990 mL | 0.4948 mL | 0.9895 mL | 2.4739 mL | |
| 20 mM | 0.0742 mL | 0.3711 mL | 0.7422 mL | 1.8554 mL | |
| 25 mM | 0.0594 mL | 0.2969 mL | 0.5937 mL | 1.4843 mL | |
| 30 mM | 0.0495 mL | 0.2474 mL | 0.4948 mL | 1.2369 mL |
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