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  3. Fluorescent Dye
  4. Ac-VEID-AMC

Ac-VEID-AMC is a fluorescent substrate for the determination of caspase-6 and related cysteine protease activities (Ex=340-360 nm, Em=440-460 nm).

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Ac-VEID-AMC Chemical Structure

Ac-VEID-AMC Chemical Structure

CAS No. : 219137-97-0

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Ac-VEID-AMC Related Antibodies

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Description

Ac-VEID-AMC is a fluorescent substrate for the determination of caspase-6 and related cysteine protease activities (Ex=340-360 nm, Em=440-460 nm)[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Enzyme activity (rabbit osteoclasts for example)[1]:
1. Treat purified, differentiated cells by medium for 48 h (time depends on the cell).
2. Put treated cells into 4.5 mL lysis buffer.
3. Each lysate is divided into thirds, then incubate with 40 μM Ac-VEID-AMC at 37°C for 1 h.
4. Detect cleavage of fluorescent substrates (Ex=340-360 nm, Em=440-460 nm)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ac-VEID-AMC Related Antibodies
Molecular Weight

673.71

Formula

C32H43N5O11
CAS No.
Emission (Em)

460
Excitation (Ex)

380
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Ac-VEID-AMC Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ac-VEID-AMC219137-97-0Fluorescent Dyecaspase-6protease activityfluorescent substrateInhibitorinhibitorinhibit

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Ac-VEID-AMC
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HY-P2065
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