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  4. Ac-VEID-AMC

Ac-VEID-AMC is a fluorescent substrate for the determination of caspase-6 and related cysteine protease activities (Ex=340-360 nm, Em=440-460 nm).

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Ac-VEID-AMC

Ac-VEID-AMC Chemical Structure

CAS No. : 219137-97-0

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Based on 1 publication(s) in Google Scholar

Ac-VEID-AMC Related Antibodies

Top Publications Citing Use of Products

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

Ac-VEID-AMC is a fluorescent substrate for the determination of caspase-6 and related cysteine protease activities (Ex=340-360 nm, Em=440-460 nm)[1].

In Vitro

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Enzyme activity (rabbit osteoclasts for example)[1]:
1. Treat purified, differentiated cells by medium for 48 h (time depends on the cell).
2. Put treated cells into 4.5 mL lysis buffer.
3. Each lysate is divided into thirds, then incubate with 40 μM Ac-VEID-AMC at 37°C for 1 h.
4. Detect cleavage of fluorescent substrates (Ex=340-360 nm, Em=440-460 nm)

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ac-VEID-AMC Related Antibodies
Molecular Weight

673.71

Formula

C32H43N5O11
CAS No.
Appearance

Solid

Color

White to off-white

Emission (Em)

460
Excitation (Ex)

380
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Sealed storage, away from moisture

Powder -80°C 2 years
-20°C 1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (37.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.4843 mL 7.4216 mL 14.8432 mL
5 mM 0.2969 mL 1.4843 mL 2.9686 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.4843 mL 7.4216 mL 14.8432 mL 37.1080 mL
5 mM 0.2969 mL 1.4843 mL 2.9686 mL 7.4216 mL
10 mM 0.1484 mL 0.7422 mL 1.4843 mL 3.7108 mL
15 mM 0.0990 mL 0.4948 mL 0.9895 mL 2.4739 mL
20 mM 0.0742 mL 0.3711 mL 0.7422 mL 1.8554 mL
25 mM 0.0594 mL 0.2969 mL 0.5937 mL 1.4843 mL
30 mM 0.0495 mL 0.2474 mL 0.4948 mL 1.2369 mL
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Ac-VEID-AMC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ac-VEID-AMC219137-97-0Fluorescent Dyecaspase-6protease activityfluorescent substrateInhibitorinhibitorinhibit

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