Cresyl Violet perchlorate
Based on 2 publication(s) in Google Scholar
Cresyl Violet perchlorate is a red fluorescent stain, which can be used to stain neurons.
For research use only. We do not sell to patients.
- CAS No.: 41830-80-2
- Formula: C16H12ClN3O5
- Molecular Weight:361.74
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocol
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Cresyl Violet perchlorate
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Biological Activity
The estimated total number of SG neurons is 27,485±3251 and 26,705±1823 in the PV and Cresyl Violet perchlorate (CV) stained sections, respectively. There is no significant difference between them (p=0.552). Therefore, Cresyl Violet perchlorate staining is simpler and more cost effective when estimates neuronal number. Although PV stains spiral ganglion neurons (SGNs) with a greater intensity and provides a functional status, its tedious protocol limits its use for quantification. Total RC volume is estimated using probe and it is found that an average RC volume of 2.162±0.35 mm3 and 1.82±0.33 mm3 in Cresyl Violet perchlorate stained and PV immunostained sections, respectively. Volume of neurons is estimated using nucleator probe and it is 3487.63±951 μm3 and 3740.1±784 μm3 in CV stained and PV immunostained sections, respectively. Similarly, volume of neuronal nucleus is also estimated using nucleator probe and it is found to be 131.68±50 μm3 and 126.51±33 μm3 in CV stained and PV immunostained sections, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 41830-80-2
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Molecular Weight 361.74
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Formula C16H12ClN3O5
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SMILES
NC1=CC2=[O+]C3=C(C4=CC=CC=C4C(N)=C3)N=C2C=C1.O=Cl(=O)([O-])=O
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Synonyms
Oxazine 9 perchlorate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (2)
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Journal Impact Factor
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Most Recent
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Immunity
Disruption of the Na+/K+-ATPase-purinergic P2X7 receptor complex in microglia promotes stress-induced anxiety. [Abstract]2024 Mar 12;57(3):495-512.e11. PMID: 38395698 -
Cerebellum
NR1 Splicing Variant NR1a in Cerebellar Granule Neurons Constitutes a Better Motor Learning in the Mouse. [Abstract]2024 Jun;23(3):1112-1120. PMID: 37880519
Solvent & Solubility
DMSO : 250 mg/mL (691.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
Cochlear sections containing SGNs are placed in 24 wells plates containing PBS (pH 7.4) and stored at 4°C. The sections are then used for Cresyl Violet perchlorate (CV) and immunohistochemical (IHC) staining. Every 7th section is stained with Cresyl Violet perchlorate (1%), dehydrated with ascending grades of alcohol, cleared with xylene, mounted with DPX and observed under microscope. Approximately 12-13 Cresyl Violet perchlorate stained sections from each specimen are used for stereology. None of these cases show any histopathological changes under the light microscope. Estimation of the total volume of the Rosenthal canal (RC), total number of SGNs (optical fractionator probe) and the volume of the soma and their nucleus (nucleator probe) is done with software[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
References
Complete Stock Solution Preparation Table
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7644 mL | 13.8221 mL | 27.6442 mL | 69.1104 mL |
| 5 mM | 0.5529 mL | 2.7644 mL | 5.5288 mL | 13.8221 mL | |
| 10 mM | 0.2764 mL | 1.3822 mL | 2.7644 mL | 6.9110 mL | |
| 15 mM | 0.1843 mL | 0.9215 mL | 1.8429 mL | 4.6074 mL | |
| 20 mM | 0.1382 mL | 0.6911 mL | 1.3822 mL | 3.4555 mL | |
| 25 mM | 0.1106 mL | 0.5529 mL | 1.1058 mL | 2.7644 mL | |
| 30 mM | 0.0921 mL | 0.4607 mL | 0.9215 mL | 2.3037 mL | |
| 40 mM | 0.0691 mL | 0.3456 mL | 0.6911 mL | 1.7278 mL | |
| 50 mM | 0.0553 mL | 0.2764 mL | 0.5529 mL | 1.3822 mL | |
| 60 mM | 0.0461 mL | 0.2304 mL | 0.4607 mL | 1.1518 mL | |
| 80 mM | 0.0346 mL | 0.1728 mL | 0.3456 mL | 0.8639 mL | |
| 100 mM | 0.0276 mL | 0.1382 mL | 0.2764 mL | 0.6911 mL |
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