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  3. Cresyl Violet perchlorate

Cresyl Violet perchlorate  (Synonyms: Oxazine 9 perchlorate)

Cat. No.: HY-101889
Handling Instructions

Cresyl Violet perchlorate is a red fluorescent stain, which can be used to stain neurons.

For research use only. We do not sell to patients.

Cresyl Violet perchlorate Chemical Structure

Cresyl Violet perchlorate Chemical Structure

CAS No. : 41830-80-2

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Description

Cresyl Violet perchlorate is a red fluorescent stain, which can be used to stain neurons.

In Vitro

The estimated total number of SG neurons is 27,485±3251 and 26,705±1823 in the PV and Cresyl Violet perchlorate (CV) stained sections, respectively. There is no significant difference between them (p=0.552). Therefore, Cresyl Violet perchlorate staining is simpler and more cost effective when estimates neuronal number. Although PV stains spiral ganglion neurons (SGNs) with a greater intensity and provides a functional status, its tedious protocol limits its use for quantification. Total RC volume is estimated using probe and it is found that an average RC volume of 2.162±0.35 mm3 and 1.82±0.33 mm3 in Cresyl Violet perchlorate stained and PV immunostained sections, respectively. Volume of neurons is estimated using nucleator probe and it is 3487.63±951 μm3 and 3740.1±784 μm3 in CV stained and PV immunostained sections, respectively. Similarly, volume of neuronal nucleus is also estimated using nucleator probe and it is found to be 131.68±50 μm3 and 126.51±33 μm3 in CV stained and PV immunostained sections, respectively[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

361.74

Formula

C16H12ClN3O5

CAS No.
SMILES

NC1=CC2=[O+]C3=C(C4=CC=CC=C4C(N)=C3)N=C2C=C1.O=Cl(=O)([O-])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (691.10 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7644 mL 13.8221 mL 27.6442 mL
5 mM 0.5529 mL 2.7644 mL 5.5288 mL
View the Complete Stock Solution Preparation Table
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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Purity & Documentation
References
Cell Assay
[1]

Cochlear sections containing SGNs are placed in 24 wells plates containing PBS (pH 7.4) and stored at 4°C. The sections are then used for Cresyl Violet perchlorate (CV) and immunohistochemical (IHC) staining. Every 7th section is stained with Cresyl Violet perchlorate (1%), dehydrated with ascending grades of alcohol, cleared with xylene, mounted with DPX and observed under microscope. Approximately 12-13 Cresyl Violet perchlorate stained sections from each specimen are used for stereology. None of these cases show any histopathological changes under the light microscope. Estimation of the total volume of the Rosenthal canal (RC), total number of SGNs (optical fractionator probe) and the volume of the soma and their nucleus (nucleator probe) is done with software[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Complete Stock Solution Preparation Table

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7644 mL 13.8221 mL 27.6442 mL 69.1104 mL
5 mM 0.5529 mL 2.7644 mL 5.5288 mL 13.8221 mL
10 mM 0.2764 mL 1.3822 mL 2.7644 mL 6.9110 mL
15 mM 0.1843 mL 0.9215 mL 1.8429 mL 4.6074 mL
20 mM 0.1382 mL 0.6911 mL 1.3822 mL 3.4555 mL
25 mM 0.1106 mL 0.5529 mL 1.1058 mL 2.7644 mL
30 mM 0.0921 mL 0.4607 mL 0.9215 mL 2.3037 mL
40 mM 0.0691 mL 0.3456 mL 0.6911 mL 1.7278 mL
50 mM 0.0553 mL 0.2764 mL 0.5529 mL 1.3822 mL
60 mM 0.0461 mL 0.2304 mL 0.4607 mL 1.1518 mL
80 mM 0.0346 mL 0.1728 mL 0.3456 mL 0.8639 mL
100 mM 0.0276 mL 0.1382 mL 0.2764 mL 0.6911 mL
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Cresyl Violet perchlorate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Product Name:
Cresyl Violet perchlorate
Cat. No.:
HY-101889
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