Fura Red 

Fura Red is a Ca²⁺-sensitive fluorescent dye, which decreases in fluorescence with rising [Ca²⁺]^[1].

Guide^[2] (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Cells:
1. Resuspend the cells in Hanks' Balanced Salt Solution (HBSS), add 1 μM Fura Red AM, supplement with 0.01% Pluronic F127 unless otherwise stated, and incubate in a 37°C water bath for 30 minutes.
2. Wash the cells in HEPES-buffered salt solution (HBSS) containing 1 mmol/L calcium chloride, 0.5 mmol/L magnesium chloride, 0.1% bovine serum albumin, and 10 mmol/L HEPES, then resuspend them in the same HEPES-buffered salt solution supplemented with the vital dye SYTOX Green.
3. Equilibrate the cells in a 37°C water bath for at least 10 minutes.
4. Analyze the samples on a flow cytometer, fluorescence microscope, or fluorescence microplate reader. Ratiometric analysis of Fura Red is performed by excitation with a violet laser (406 nm) and a green laser (502 nm).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

944.87

C₄₁H₄₄N₄O₂₀S

149732-62-7

652

471

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Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Floto RA, et al. IgG-induced Ca2+ oscillations in differentiated U937 cells; a study using laser scanning confocal microscopy and co-loaded fluo-3 and fura-red fluorescent probes. Cell Calcium. 1995 Nov;18(5):377-89.  [Content Brief]

  [2]. Wendt ER, et al. Ratiometric analysis of fura red by flow cytometry: a technique for monitoring intracellular calcium flux in primary cell subsets. PLoS One. 2015 Apr 2;10(3):e0119532.  [Content Brief]