2. Academic Validation
  3. Circular RNA circPTPN3 facilitates duck hepatitis A virus type 3 (DHAV-3) infection by reducing miR-130b-5p function in duck

Circular RNA circPTPN3 facilitates duck hepatitis A virus type 3 (DHAV-3) infection by reducing miR-130b-5p function in duck

  • Int J Biol Macromol. 2025 Dec;334(Pt 2):149132. doi: 10.1016/j.ijbiomac.2025.149132.
Ying Liu 1,  Shaofei Li 2,  Xiang Mei 3,  Shujie Tian 4,  Junting Cao 5,  Shuaiqin Wang 6,  Shuisheng Hou 7,  Xia Wang 8,  Yunsheng Zhang 9
Affiliations

Affiliations

  • 1 College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: [email protected].
  • 2 College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: [email protected].
  • 3 College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: [email protected].
  • 4 College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: [email protected].
  • 5 Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. Electronic address: [email protected].
  • 6 Key Laboratory of Feed Biotechnology of Ministry of Agriculture and Rural Affairs, Institute of Feed Research, Chinese Academy of Agricultural Sciences, Beijing, 100081, China. Electronic address: [email protected].
  • 7 Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China. Electronic address: [email protected].
  • 8 College of Animal Science and Technology, Northwest A&F University, Yangling, 712100, Shaanxi, China. Electronic address: [email protected].
  • 9 Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, 100193, China; Inner Mongolia Key Laboratory of Duck Breeding, Inner Mongolia Chifeng, 024207, China. Electronic address: [email protected].
PMID: 41265597 DOI: 10.1016/j.ijbiomac.2025.149132
Abstract

Duck hepatitis A virus type 3 (DHAV-3) causes severe economic losses in the duck industry, yet the regulatory roles of circular RNAs (circRNAs) during Infection remain unclear. This study combined transcriptome Sequencing and functional analyses to investigate circRNA expression and mechanisms in DHAV-3 Infection. We identified 44 differentially expressed circRNAs in duck livers post-infection, among which circPTPN3 was the most significantly upregulated. Gain- and loss-of-function experiments confirmed that circPTPN3 promotes DHAV-3 replication. Mechanistically, circPTPN3 was found to act as a Sponge for miR-130b-5p, a miRNA with Antiviral effects, thereby alleviating its suppression of pro-viral genes (e.g., ZC2HC1A, NMNAT2, and GPR65). These results reveal a novel circPTPN3/miR-130b-5p regulatory axis that facilitates DHAV-3 replication, providing new insights into host-virus interactions and potential therapeutic targets for Antiviral strategies.

Keywords

DHAV-3; circPTPN3; miR-130b-5p.

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