1. GPCR/G Protein
    Metabolic Enzyme/Protease
  2. Glucocorticoid Receptor
    Endogenous Metabolite
  3. Hydrocortisone

Hydrocortisone (Synonyms: Cortisol)

Cat. No.: HY-N0583 Purity: 99.90%
Handling Instructions

Hydrocortisone is a steroid hormone or glucocorticoid secreted by the adrenal cortex.

For research use only. We do not sell to patients.

Hydrocortisone Chemical Structure

Hydrocortisone Chemical Structure

CAS No. : 50-23-7

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10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
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Estimated Time of Arrival: December 31
5 g USD 96 In-stock
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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Hydrocortisone:

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Publications Citing Use of MCE Hydrocortisone

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  • References

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Description

Hydrocortisone is a steroid hormone or glucocorticoid secreted by the adrenal cortex.

In Vitro

Hydrocortisone (50 nM) shows a dose-dependent down-regulation of GR transcript in hCMEC/D3 cells. Hydrocortisone supplementation of the serum-reduced cell differentiation medium leads to a significant increase in TER across the hCMEC/D3 monolayer[1]. Hydrocortisone-treated Dendritic cells (DCs) show a decreased expression of MHC II molecules, the costimulatory molecule CD86, and the DC-specific marker CD83, as well as a strongly reduced IL-12 secretion. Hydrocortisone-treated DCs inhibit production of IFN-γ but induce an increased release of IL-4 and no change in IL-5[2]. Hydrocortisone reduces postischemic oxidative stress, perfusion pressure, and transudate formation. Hydrocortisone inhibits postischemic shedding of syndecan-1, heparan sulfate, and hyaluronan as is release of histamine from resident mast cells[3].

Clinical Trial
Molecular Weight

362.46

Formula

C₂₁H₃₀O₅

CAS No.

50-23-7

SMILES
Shipping

Room temperature in continental US; may vary elsewhere

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 31 mg/mL (85.53 mM)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7589 mL 13.7946 mL 27.5893 mL
5 mM 0.5518 mL 2.7589 mL 5.5179 mL
10 mM 0.2759 mL 1.3795 mL 2.7589 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (6.90 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.5 mg/mL (6.90 mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.5 mg/mL (6.90 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[1]

Cells are plated on top of collagen IV-coated transwell chambers for six-well plates (24 mm diameter, membrane material: polyethylene terephthalate (PET), 0.4 μm pores, pore density 1.6×106 cm2) at densities of 2.5×104 cells cm2 per well. When they have reached confluence at day 5, the different experimental sets of cells are transferred to differentiation medium containing reduced amounts of FCS and treated with TNFα or hydrocortisone as indicated.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.90%

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Hydrocortisone
Cat. No.:
HY-N0583
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