Methylene blue (purity≥70%) (Synonyms: Basic Blue 9 (purity≥70%); CI-52015 (purity≥70%); Methylthioninium chloride (purity≥70%))

Name: Methylene blue (purity≥70%)
Brand: MedChemExpress
SKU: HY-14536A
Rating: 4.5 (0 reviews)

Cat. No.: HY-14536A Purity: 95.0%: Data Sheet
COA

SDS
Handling Instructions
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Methylene blue (purity≥70%) is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue (purity≥70%) is a vasopressor and is often used as a dye in several medical procedures. Methylene blue (purity≥70%) through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue (purity≥70%) is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue (purity≥70%) is a Tau aggregation inhibitor. Methylene blue reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation.

For research use only. We do not sell to patients.

Methylene blue (purity≥70%) Chemical Structure

CAS No. : 61-73-4

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	In-stock	Estimated Time of Arrival: December 31
10 g	In-stock	Estimated Time of Arrival: December 31
25 g	In-stock	Estimated Time of Arrival: December 31
50 g	In-stock	Estimated Time of Arrival: December 31
100 g	In-stock	Estimated Time of Arrival: December 31
> 100 g	Get quote

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Customer Review

Based on 1 Customer Validation

Other Forms of Methylene blue (purity≥70%):

Methylene Blue In-stock
Methylene blue hydrate In-stock

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MAO-A MAO-B

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nNOS iNOS eNOS

Biological Activity
Purity & Documentation
References
Customer Review

Description

Cellular Effect

Cell Line	Type	Value	Description	References
B16-F10	IC₅₀	7.02 3 Compound: MB	Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay	[PMID: 34871840]
B16-F10	IC₅₀	5.48 3 Compound: MB	Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in light irradiation and measured for 48 hrs by MTT assay Antiproliferative activity against mouse B16-F10 cells assessed as cell viability in light irradiation and measured for 48 hrs by MTT assay	[PMID: 34871840]
HepG2	IC₅₀	6 3 Compound: MB	Anticancer activity against human HepG2 cells Anticancer activity against human HepG2 cells	[PMID: 33460833]
HepG2	IC₅₀	<1 3 Compound: Methylene blue	Antimalarial activity against sporozoite stage of Plasmodium yoelii assessed as invasion of human HepG2 cells expressing CD81 incubated for 2 hrs prior to inoculation measured after 1 hr by immunofluorescence assay in presence of penicillin/streptomycin Antimalarial activity against sporozoite stage of Plasmodium yoelii assessed as invasion of human HepG2 cells expressing CD81 incubated for 2 hrs prior to inoculation measured after 1 hr by immunofluorescence assay in presence of penicillin/streptomycin	[PMID: 23927658]
HT-29	IC₅₀	>10 3 Compound: MB	Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 24 hrs in dark by MTT assay Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 24 hrs in dark by MTT assay	[PMID: 32267685]
HT-29	IC₅₀	9.2 3 Compound: MB	Cytotoxicity against human HT-29 cells assessed as reduction in cell viability preincubated for 1 hr followed by light irradiation at 3.2 J/cm2 for 30 sec and measured after 24 hrs by MTT assay Cytotoxicity against human HT-29 cells assessed as reduction in cell viability preincubated for 1 hr followed by light irradiation at 3.2 J/cm2 for 30 sec and measured after 24 hrs by MTT assay	[PMID: 32267685]
Fibroblast	EC₅₀	43 1 Compound: MB	Inhibition of erastin-induced cell death in FRDA patient-derived fibroblast assessed as depletion of cellular ATP incubated for 12 hrs followed by erastin stimulation and measured after by luciferase-linked ATPase enzymatic assay Inhibition of erastin-induced cell death in FRDA patient-derived fibroblast assessed as depletion of cellular ATP incubated for 12 hrs followed by erastin stimulation and measured after by luciferase-linked ATPase enzymatic assay	[PMID: 33214825]
MCF7	IC₅₀	14.64 3 Compound: MB	Antiproliferative activity against human MCF7 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as cell viability in dark incubated for 48 hrs by MTT assay	[PMID: 34871840]
MCF7	IC₅₀	10.58 3 Compound: MB	Antiproliferative activity against human MCF7 cells assessed as cell viability in light irradiation and measured after 48 hrs by MTT assay Antiproliferative activity against human MCF7 cells assessed as cell viability in light irradiation and measured after 48 hrs by MTT assay	[PMID: 34871840]
Lymphocyte	EC₅₀	88 1 Compound: MB	Anti-ferroptotic activity in FRDA patient-derived Lymphocyte assessed as reduction in RSL3-induced lipid peroxidation incubated for overnight followed by RSL3 stimulation and measured after 90 mins by FACS analysis Anti-ferroptotic activity in FRDA patient-derived Lymphocyte assessed as reduction in RSL3-induced lipid peroxidation incubated for overnight followed by RSL3 stimulation and measured after 90 mins by FACS analysis	[PMID: 33214825]

In Vitro

Guidelines (The following is our recommended protocol. This protocol is provided for reference only and should be modified according to your specific needs).
1. Sample treatment
1.1 For paraffin sections: dewax and rehydrate as usual.
1.2 For frozen sections: distilled water for 2 min.
1.3 For cultured cells: Fix with 4% paraformaldehyde. Wash with distilled water for 2 min. Replace with fresh distilled water and wash for another 2 min.
2. Methylene blue staining
2.1 Stain with 0.1% Methylene blue for 2 min.
2.2 Wash thoroughly with distilled water, observe and take photos.
2.3 Staining results:
Tissue or cells: blue

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

319.86

Formula

C₁₆H₁₈ClN₃S

CAS No.

61-73-4

Appearance

Solid

Color

Brown to black

SMILES

CN(C)C1=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C2C=C1.[Cl-]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 20 mg/mL (62.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.1264 mL	15.6318 mL	31.2637 mL
5 mM	0.6253 mL	3.1264 mL	6.2527 mL
10 mM	0.3126 mL	1.5632 mL	3.1264 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

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Concentration _(final)

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 95.0%

Select Batch:

Data Sheet (273 KB) SDS (397 KB)

COA (247 KB) HNMR (116 KB) LCMS (233 KB)

Handling Instructions (2659 KB)

References

[1]. Klamer D, et, al. Phencyclidine-induced behaviour in mice prevented by methylene blue. Basic Clin Pharmacol Toxicol. 2004 Feb;94(2):65-72. [Content Brief]

[2]. Hochgräfe K, et, al. Preventive methylene blue treatment preserves cognition in mice expressing full-length pro-aggregant human Tau. Acta Neuropathol Commun. 2015 May 10;3:25. [Content Brief]

[3]. Fenn AM, et, al. Methylene blue attenuates traumatic brain injury-associated neuroinflammation and acute depressive-like behavior in mice. J Neurotrauma. 2015 Jan 15;32(2):127-38. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.1264 mL	15.6318 mL	31.2637 mL	78.1592 mL
	5 mM	0.6253 mL	3.1264 mL	6.2527 mL	15.6318 mL
	10 mM	0.3126 mL	1.5632 mL	3.1264 mL	7.8159 mL
	15 mM	0.2084 mL	1.0421 mL	2.0842 mL	5.2106 mL
	20 mM	0.1563 mL	0.7816 mL	1.5632 mL	3.9080 mL
	25 mM	0.1251 mL	0.6253 mL	1.2505 mL	3.1264 mL
	30 mM	0.1042 mL	0.5211 mL	1.0421 mL	2.6053 mL
	40 mM	0.0782 mL	0.3908 mL	0.7816 mL	1.9540 mL
	50 mM	0.0625 mL	0.3126 mL	0.6253 mL	1.5632 mL
	60 mM	0.0521 mL	0.2605 mL	0.5211 mL	1.3027 mL

Methylene blue (purity≥70%) Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Methylene blue (purity≥70%) (Synonyms: Basic Blue 9 (purity≥70%); CI-52015 (purity≥70%); Methylthioninium chloride (purity≥70%))

Customer Review

Other Forms of Methylene blue (purity≥70%):

Methylene blue (purity≥70%) Related Antibodies

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All Monoamine Oxidase Isoform Specific Products:

View All NO Synthase Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Methylene blue (purity≥70%) Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Methylene blue (purity≥70%) Related Classifications

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