Methylene blue hydrate
Based on 27 publication(s) in Google Scholar
Methylene blue (Basic Blue 9) hydrate is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue is a vasopressor and is often used as a dye in several medical procedures. Methylene blue hydrate through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue hydrate is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue hydrate is a Tau aggregation inhibitor. Methylene blue hydrate reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation.
For research use only. We do not sell to patients.
- CAS No.: 122965-43-9
- Formula: C16H18N3S.Cl.xH2O
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Methylene blue hydrate
More- Nat Commun. 2024 Nov 27;15(1):10303. [Abstract]
- ACS Nano. 2026 Jun 30;20(25):18058-18072. [Abstract]
- Redox Biol. 2020 Sep;36:101601. [Abstract]
- Gut Microbes. 2025 Dec 31;17(1):2599543. [Abstract]
- J Nanobiotechnology. 2025 Jul 4;23(1):485. [Abstract]
- Theranostics. 2021 Oct 17;11(20):9884-9903. [Abstract]
- Biomaterials. 2023 Feb:293:121988. [Abstract]
- Chem Eng J. 2025 Nov 24;526:171230.
- Phytomedicine. 2025 Oct 20:148:157423. [Abstract]
- Nano Res. 2026 Feb 6.
- Phytother Res. 2023 Apr;37(4):1405-1421. [Abstract]
- Cell Rep. 2025 Nov 25;44(11):116520. [Abstract]
- Cell Rep. 2024 Feb 13;43(2):113779. [Abstract]
- Nanophotonics. 2025 Nov 25;14(27):4993-5001. [Abstract]
- J Environ Sci. 2025 Dec 25.
- Bioengineering (Basel). 2025 Oct 27;12(11):1164. [Abstract]
- Chemistry. 2026 May;8(5):65.
- Biomed Res Int. 2021 May 14:2021:5565748. [Abstract]
- Exp Ther Med. 2019 Nov;18(5):4049-4057. [Abstract]
- World J Gastrointest Surg. 2023 Jun 27;15(6):1068-1079. [Abstract]
- Hematology. 2022 Dec;27(1):43-52. [Abstract]
- Patent. US20240325538A1.
- Patent. US20240238271A1.
- Patent. US20230226111A1.
- Research Square Print. 2023 Feb 21.
- Curr Res Virol Sci. 2022;3:100019. [Abstract]
- Evid Based Complement Alternat Med. 2020 Mar 10;2020:3524641. [Abstract]
Customer Validation & Images
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Gel Electrophoresis
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Gel Electrophoresis
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Gel Electrophoresis
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Cell Migration/Invasion Assay
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WB
Biological Activity
Methylene blue hydrate (20 and 40 mg/kg; p.o.; daily, for 6 months; CaMKIIα-tTA transactivator mice) preserves cognition in mice expressing full-length pro-aggregant human Tau[2].
Methylene blue hydrate (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces TBI-induced edema and neuroinflammation and reduces acute depression-like behavior[3].
Methylene blue hydrate (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces the percentage of inflammatory factor[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male NMRI mice[1]
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Dosage:50 and 100 mg/kg
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Administration:Intraperitoneal injection; once, for 25 minutes
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Result:Reduced the prepulse inhibition and reduced the increase in locomotor activity caused by phencyclidine (PCP).
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Animal Model:CaMKIIα-tTA transactivator mice[2]
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Dosage:20 and 40 mg/kg
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Administration:Oral administration; daily, for 6 months
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Result:Inhibited Tau aggregation in CaMKIIα-tTA transactivator mice.
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Animal Model:TBI-treated male BALB/c mice[3]
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Dosage:2 mg/kg
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Administration:Intravenous injection; once, for 1 day
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Result:Decreased the level of CD14, IL-1β, TNF-α, and CCL2 mRNA.
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Animal Model:TBI-treated male BALB/c mice[3]
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Dosage:2 mg/kg
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Administration:Intravenous injection; once, for 1 day
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Result:Reduced the percentage of myeloid (CD11b+/GR1+) cells, reduced IL-1β and enhanced IL-10 expression in microglia.
Chemical Information
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CAS No. 122965-43-9
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Appearance Solid
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Formula C16H18N3S.Cl.xH2O
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Color Light green to green
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SMILES
CN(C1=CC2=[S+]C3=C(C=CC(N(C)C)=C3)N=C2C=C1)C.[x].[Cl-].O
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Synonyms
Basic Blue 9 hydrate; CI-52015 hydrate; Methylthioninium chloride hydrate
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (27)
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Journal Impact Factor
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Most Recent
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Nat Commun
DEAD-box RNA helicase 10 is required for 18S rRNA maturation by controlling the release of U3 snoRNA from pre-rRNA in embryonic stem cells. [Abstract]2024 Nov 27;15(1):10303. PMID: 39604362
Methylene blue hydrate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Nov 27;15(1):10303. [Abstract]
Northern blot analysis of pre-rRNA intermediates using 5'ETS-1, 5'ETS-2, 5'ETS and ITS1-29 probes in DDX10-AID mESCs with IAA treatment (0 h, 6 h, 24 h, and 48 h) or treated with IAA for 48 h followed by 48 h of washing. MB represents methylene blue staining. Experiments were repeated three times independently with similar results. Membranes were stained with Methylene Blue stain solution (0.03% Methylene Blue in 0.3 M sodium acetate (pH 5.0-5.5)), and then RNA was crosslinked onto the membrane using UV light.
Methylene blue hydrate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Nov 27;15(1):10303. [Abstract]
Northern blot analysis of pre-rRNA intermediate levels in DDX10-AID (+ OsTir1) mESCs overexpressing DDX10FL, DDX10ΔHBD, DDX10ΔHCD or DDX10ΔHD at 0 h and 48 h after IAA treatment. MB represents Methylene Blue staining. Experiments were repeated three times independently with similar results. Source data are provided as a Source Data file. Membranes were stained with Methylene Blue stain solution (0.03% Methylene Blue in 0.3 M sodium acetate (pH 5.0-5.5)), and then RNA was crosslinked onto the membrane using UV light.
Methylene blue hydrate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Nov 27;15(1):10303. [Abstract]
Northern blot analysis of pre-rRNA intermediates in DDX10-AID (+ OsTir1) mESCs overexpressing NUP98-DDX10 or DDX10, treated with IAA at different time points (0 h, 24 h, and 48 h). MB represents Methylene Blue staining. Experiments were repeated two times independently with similar results. Source data are provided as a Source Data file. Membranes were stained with Methylene Blue stain solution (0.03% Methylene Blue in 0.3 M sodium acetate (pH 5.0-5.5)), and then RNA was crosslinked onto the membrane using UV light.
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ACS Nano
Keratinocyte-Derived Apoptotic Nanovehicles Reprogram Cutaneous Dendritic Cells to Ameliorate Psoriasis. [Abstract]2026 Jun 30;20(25):18058-18072. PMID: 42301004 -
Redox Biol
An effective inactivant based on singlet oxygen-mediated lipid oxidation implicates a new paradigm for broad-spectrum antivirals. [Abstract]2020 Sep;36:101601. PMID: 32535542 -
Gut Microbes
Helicobacter pylori infection aggravates hepatic steatosis by lactylation-driven WTAP-mediated m6A modification. [Abstract]2025 Dec 31;17(1):2599543. PMID: 41384837 -
J Nanobiotechnology
SIRT5-modified human umbilical cord mesenchymal stem cells loaded with antioxidant polydopamine nanozyme enhance parpi resistance in ovarian cancer via fatty acid metabolism reprogramming. [Abstract]2025 Jul 4;23(1):485. PMID: 40616128 -
Theranostics
Establishment of tumor inflammasome clusters with distinct immunogenomic landscape aids immunotherapy. [Abstract]2021 Oct 17;11(20):9884-9903. PMID: 34815793
Methylene blue hydrate purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Oct 17;11(20):9884-9903. [Abstract]
Migration analysis of THP-1, co-cultured with cancer cells that were pre-treated with Methylene Blue.
Methylene blue hydrate purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Oct 17;11(20):9884-9903. [Abstract]
Western blotting analysis of PD-L1 expression in THP-1 which was co-cultured with cancer cells pre-treated with Methylene Blue.
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Biomaterials
Manganese-enriched photonic/catalytic nanomedicine augments synergistic anti-TNBC photothermal/nanocatalytic/immuno-therapy via activating cGAS-STING pathway. [Abstract]2023 Feb:293:121988. PMID: 36580716 -
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Phytomedicine
Arnicolide C induces ROS-mediated modulation of PI3K/Akt and MAPK pathways to suppress MYC in hepatocellular carcinoma. [Abstract]2025 Oct 20:148:157423. PMID: 41138574 -
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Phytother Res
Carnosol inhibits KGN cells oxidative stress and apoptosis and attenuates polycystic ovary syndrome phenotypes in mice through Keap1-mediated Nrf2/HO-1 activation. [Abstract]2023 Apr;37(4):1405-1421. PMID: 36786429 -
Cell Rep
WDFY2 promotes MRN complex formation required for homologous recombination-mediated DNA repair. [Abstract]2025 Nov 25;44(11):116520. PMID: 41196680 -
Cell Rep
The ARID1A-METTL3-m6A axis ensures effective RNase H1-mediated resolution of R-loops and genome stability. [Abstract]2024 Feb 13;43(2):113779. PMID: 38358891 -
Nanophotonics
2025 Nov 25;14(27):4993-5001. PMID: 41426083 -
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Bioengineering (Basel)
2025 Oct 27;12(11):1164. PMID: 41301120 -
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Biomed Res Int
Gentiopicroside Produces Endothelium-Independent Vasodilation by Deactivating the PI3K/Akt/Rho-Kinase Pathway in Isolated Rat Thoracic Aorta. [Abstract]2021 May 14:2021:5565748. PMID: 34095301 -
Exp Ther Med
MicroRNA-139-5p regulates chronic inflammation by suppressing nuclear factor-κB activity to inhibit cell proliferation and invasion in colorectal cancer. [Abstract]2019 Nov;18(5):4049-4057. PMID: 31616518 -
World J Gastrointest Surg
Impact of interstitial cells of Cajal on slow wave and gallbladder contractility in a guinea pig model of acute cholecystitis. [Abstract]2023 Jun 27;15(6):1068-1079. PMID: 37405098 -
Hematology
Downregulation of Smad4 expression confers chemoresistance against imatinib mesylate to chronic myeloid leukemia K562 cells. [Abstract]2022 Dec;27(1):43-52. PMID: 34957936 -
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Curr Res Virol Sci
Identification of broad anti-coronavirus chemical agents for repurposing against SARS-CoV-2 and variants of concern. [Abstract]2022;3:100019. PMID: 35072124 -
Evid Based Complement Alternat Med
Acupuncture at Gastric Back-Shu and Front-Mu Acupoints Enhances Gastric Motility via the Inhibition of the Glutamatergic System in the Hippocampus. [Abstract]2020 Mar 10;2020:3524641. PMID: 32215036
Solvent & Solubility
DMSO : 7.14 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : 2 mg/mL (ultrasonic and warming and heat to 60°C)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Klamer D, et, al. Phencyclidine-induced behaviour in mice prevented by methylene blue. Basic Clin Pharmacol Toxicol. 2004 Feb;94(2):65-72. [Content Brief]
[2]. Hochgräfe K, et, al. Preventive methylene blue treatment preserves cognition in mice expressing full-length pro-aggregant human Tau. Acta Neuropathol Commun. 2015 May 10;3:25. [Content Brief]
[3]. Fenn AM, et, al. Methylene blue attenuates traumatic brain injury-associated neuroinflammation and acute depressive-like behavior in mice. J Neurotrauma. 2015 Jan 15;32(2):127-38. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Methylene blue hydrate Related Classifications
HY-D0958 Related Classifications
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