2. GPCR/G Protein Neuronal Signaling Immunology/Inflammation Cell Cycle/DNA Damage Cytoskeleton
  3. Guanylate Cyclase Monoamine Oxidase NO Synthase Microtubule/Tubulin
  4. Methylene blue hydrate

Methylene blue hydrate  (Synonyms: Basic Blue 9 hydrate; CI-52015 hydrate; Methylthioninium chloride hydrate)

Cat. No.: HY-D0958
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Methylene blue (Basic Blue 9) hydrate is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue is a vasopressor and is often used as a dye in several medical procedures. Methylene blue hydrate through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue hydrate is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue hydrate is a Tau aggregation inhibitor. Methylene blue hydrate reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation.

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Methylene blue hydrate Chemical Structure

Methylene blue hydrate Chemical Structure

CAS No. : 122965-43-9

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Based on 13 publication(s) in Google Scholar

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Description

Methylene blue (Basic Blue 9) hydrate is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue is a vasopressor and is often used as a dye in several medical procedures. Methylene blue hydrate through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue hydrate is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue hydrate is a Tau aggregation inhibitor. Methylene blue hydrate reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation[1][2][3].

In Vitro

Methylene blue (Basic Blue 9) hydrate (4.5 μM; BV2 microglia) alters the immune profile of LPS-activated BV2 microglia and decreased the level of CD14, IL-1β, TNF-α, and CCL2 mRNA[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Methylene blue hydrate Related Antibodies
In Vivo

Methylene blue (Basic Blue 9) hydrate (50 and 100 mg/kg; i.p.; once, for 25 min; male NMRI mice) reduces absent prepulse inhibition[1].
Methylene blue hydrate (20 and 40 mg/kg; p.o.; daily, for 6 months; CaMKIIα-tTA transactivator mice) preserves cognition in mice expressing full-length pro-aggregant human Tau[2].
Methylene blue hydrate (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces TBI-induced edema and neuroinflammation and reduces acute depression-like behavior[3].
Methylene blue hydrate (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces the percentage of inflammatory factor[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male NMRI mice[1]
Dosage: 50 and 100 mg/kg
Administration: Intraperitoneal injection; once, for 25 minutes
Result: Reduced the prepulse inhibition and reduced the increase in locomotor activity caused by phencyclidine (PCP).
Animal Model: CaMKIIα-tTA transactivator mice[2]
Dosage: 20 and 40 mg/kg
Administration: Oral administration; daily, for 6 months
Result: Inhibited Tau aggregation in CaMKIIα-tTA transactivator mice.
Animal Model: TBI-treated male BALB/c mice[3]
Dosage: 2 mg/kg
Administration: Intravenous injection; once, for 1 day
Result: Decreased the level of CD14, IL-1β, TNF-α, and CCL2 mRNA.
Animal Model: TBI-treated male BALB/c mice[3]
Dosage: 2 mg/kg
Administration: Intravenous injection; once, for 1 day
Result: Reduced the percentage of myeloid (CD11b+/GR1+) cells, reduced IL-1β and enhanced IL-10 expression in microglia.
Clinical Trial
Formula

C16H18N3S.Cl.xH2O
CAS No.
Appearance

Solid

Color

Light green to green

SMILES

CN(C1=CC2=[S+]C3=C(C=CC(N(C)C)=C3)N=C2C=C1)C.[x].[Cl-].O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

H2O : 2 mg/mL (ultrasonic and warming and heat to 60°C)

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Methylene blue122965-43-9Basic Blue 9CI-52015Methylthioninium chlorideBasic Blue9Basic Blue-9CI52015CI 52015Guanylate CyclaseMonoamine OxidaseNO SynthaseMicrotubule/TubulinMAONitric oxide synthasesNOSvasopressordyeantinociceptionantimalarialantidepressantanxiolyticmethemoglobinemiascGMPInhibitorinhibitorinhibit

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