2. GPCR/G Protein Neuronal Signaling Immunology/Inflammation Cell Cycle/DNA Damage Cytoskeleton
  3. Guanylate Cyclase Monoamine Oxidase NO Synthase Microtubule/Tubulin
  4. Methylene blue hydrate

Methylene blue hydrate  (Synonyms: Basic Blue 9 hydrate; CI-52015 hydrate; Methylthioninium chloride hydrate)

Cat. No.: HY-D0958
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Methylene blue (Basic Blue 9) hydrate is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue is a vasopressor and is often used as a dye in several medical procedures. Methylene blue hydrate through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue hydrate is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue hydrate is a Tau aggregation inhibitor. Methylene blue hydrate reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation.

For research use only. We do not sell to patients.

CAS No. : 122965-43-9

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Based on 25 publication(s) in Google Scholar

Other Forms of Methylene blue hydrate:

Methylene blue hydrate Related Antibodies

Top Publications Citing Use of Products

    Methylene blue hydrate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Nov 27;15(1):10303.  [Abstract]

    Northern blot analysis of pre-rRNA intermediates using 5'ETS-1, 5'ETS-2, 5'ETS and ITS1-29 probes in DDX10-AID mESCs with IAA treatment (0 h, 6 h, 24 h, and 48 h) or treated with IAA for 48 h followed by 48 h of washing. MB represents methylene blue staining. Experiments were repeated three times independently with similar results. Membranes were stained with Methylene Blue stain solution (0.03% Methylene Blue in 0.3 M sodium acetate (pH 5.0-5.5)), and then RNA was crosslinked onto the membrane using UV light.

    Methylene blue hydrate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Nov 27;15(1):10303.  [Abstract]

    Northern blot analysis of pre-rRNA intermediate levels in DDX10-AID (+ OsTir1) mESCs overexpressing DDX10FL, DDX10ΔHBD, DDX10ΔHCD or DDX10ΔHD at 0 h and 48 h after IAA treatment. MB represents Methylene Blue staining. Experiments were repeated three times independently with similar results. Source data are provided as a Source Data file. Membranes were stained with Methylene Blue stain solution (0.03% Methylene Blue in 0.3 M sodium acetate (pH 5.0-5.5)), and then RNA was crosslinked onto the membrane using UV light.

    Methylene blue hydrate purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Nov 27;15(1):10303.  [Abstract]

    Northern blot analysis of pre-rRNA intermediates in DDX10-AID (+ OsTir1) mESCs overexpressing NUP98-DDX10 or DDX10, treated with IAA at different time points (0 h, 24 h, and 48 h). MB represents Methylene Blue staining. Experiments were repeated two times independently with similar results. Source data are provided as a Source Data file. Membranes were stained with Methylene Blue stain solution (0.03% Methylene Blue in 0.3 M sodium acetate (pH 5.0-5.5)), and then RNA was crosslinked onto the membrane using UV light.

    Methylene blue hydrate purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Oct 17;11(20):9884-9903.  [Abstract]

    Migration analysis of THP-1, co-cultured with cancer cells that were pre-treated with Methylene Blue.

    Methylene blue hydrate purchased from MedChemExpress. Usage Cited in: Theranostics. 2021 Oct 17;11(20):9884-9903.  [Abstract]

    Western blotting analysis of PD-L1 expression in THP-1 which was co-cultured with cancer cells pre-treated with Methylene Blue.

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    Description

    Methylene blue (Basic Blue 9) hydrate is a guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS) inhibitor. Methylene blue is a vasopressor and is often used as a dye in several medical procedures. Methylene blue hydrate through the nitric oxide syntase/guanylate cyclase signalling pathway to reduce prepulse inhibition. Methylene blue hydrate is a REDOX cycling compound and able to cross the blood-brain barrier. Methylene blue hydrate is a Tau aggregation inhibitor. Methylene blue hydrate reduces cerebral edema, attenuated microglial activation and reduced neuroinflammation[1][2][3].

    In Vitro

    Methylene blue (Basic Blue 9) hydrate (4.5 μM; BV2 microglia) alters the immune profile of LPS-activated BV2 microglia and decreased the level of CD14, IL-1β, TNF-α, and CCL2 mRNA[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Methylene blue hydrate Related Antibodies
    In Vivo

    Methylene blue (Basic Blue 9) hydrate (50 and 100 mg/kg; i.p.; once, for 25 min; male NMRI mice) reduces absent prepulse inhibition[1].
    Methylene blue hydrate (20 and 40 mg/kg; p.o.; daily, for 6 months; CaMKIIα-tTA transactivator mice) preserves cognition in mice expressing full-length pro-aggregant human Tau[2].
    Methylene blue hydrate (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces TBI-induced edema and neuroinflammation and reduces acute depression-like behavior[3].
    Methylene blue hydrate (2 mg/kg; i.v.; once, for 1 d; TBI-treated male BALB/c mice) reduces the percentage of inflammatory factor[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male NMRI mice[1]
    Dosage: 50 and 100 mg/kg
    Administration: Intraperitoneal injection; once, for 25 minutes
    Result: Reduced the prepulse inhibition and reduced the increase in locomotor activity caused by phencyclidine (PCP).
    Animal Model: CaMKIIα-tTA transactivator mice[2]
    Dosage: 20 and 40 mg/kg
    Administration: Oral administration; daily, for 6 months
    Result: Inhibited Tau aggregation in CaMKIIα-tTA transactivator mice.
    Animal Model: TBI-treated male BALB/c mice[3]
    Dosage: 2 mg/kg
    Administration: Intravenous injection; once, for 1 day
    Result: Decreased the level of CD14, IL-1β, TNF-α, and CCL2 mRNA.
    Animal Model: TBI-treated male BALB/c mice[3]
    Dosage: 2 mg/kg
    Administration: Intravenous injection; once, for 1 day
    Result: Reduced the percentage of myeloid (CD11b+/GR1+) cells, reduced IL-1β and enhanced IL-10 expression in microglia.
    Formula

    C16H18N3S.Cl.xH2O
    CAS No.
    Appearance

    Solid

    Color

    Light green to green

    SMILES

    CN(C1=CC2=[S+]C3=C(C=CC(N(C)C)=C3)N=C2C=C1)C.[x].[Cl-].O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 7.14 mg/mL (Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    H2O : 2 mg/mL (ultrasonic and warming and heat to 60°C)

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    Purity & Documentation

    SDS (393 KB)

    COA (248 KB)

    Handling Instructions (2659 KB)
    References
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    Methylene blue hydrate Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Methylene blue122965-43-9Basic Blue 9CI-52015Methylthioninium chlorideBasic Blue9Basic Blue-9CI52015CI 52015Guanylate CyclaseMonoamine OxidaseNO SynthaseMicrotubule/TubulinMAONitric oxide synthasesNOSvasopressordyeantinociceptionantimalarialantidepressantanxiolyticmethemoglobinemiascGMPInhibitorinhibitorinhibit

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